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Internal transcribed spacer sequences of nuclear ribosomal DNA resolving complex taxonomic history in the genus Vicia L.

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Abstract

The ITS1-5.8S-ITS2 region of the 18S-28S nrDNA was sequenced in 49 Vicia species and subspecies selected from nine sections of the two subgenera to resolve taxonomic contradictions. Phylogenies derived from the internal transcribed spacer (ITS) sequences strongly support the view that both Vicia faba and Vicia bithynica are distant from the Narbonensis species complex (NSC) in section Faba. V. faba is more closely aligned with sections Peregrinae and Hypechusa, whereas V. bithynica is more closely aligned with section Vicia. Six synapomorphic substitutions unique to the NSC support a separate section for the Narbonensis complex. Vicia johannis subsp. johannis was the most diverged species within the NSC with high genetic distance between V. johannis subsp. johannis and remaining species and subspecies of the NSC. These results suggest that Vicia johannis subsp. johannis should be given species rank. The neighbor-joining and parsimony based trees supported the sections Vicia (after the exclusion of Vicia lathyroides) and Cracca (after the exclusion of Vicia vicioides and Vicia hirsuta). The section Hypechusa is polyphyletic and paraphyletic based on the present ITS sequence cladistic analysis. The x = 7 as the ancestral number for the genus is supported by the present study.

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Acknowledgments

Grateful thanks are due to National Academy of Sciences, India and Shahrekord University, Iran for financial support.

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, 8818634141, Shahrekord, Iran

    Behrouz Shiran & Akram Hafizi

  2. Department of Agriculture, Payame Noor University, PO Box 19395-3697, Tehran, Iran

    Soghra Kiani

  3. International Wheat and Maize Improvement Center (CIMMYT), Km. 45, Carretera, Mex-Veracruz, El Batan, CP 56130, Texcoco, Mexico

    Deepmala Sehgal

  4. Department of Botany, University of Delhi, Delhi, 110007, India

    Tanvir ul-Hassan

  5. Amity Institute of Biotechnology, Amity University, Sector 125, Noida, U.P., India

    Manju Chaudhary & Soom Nath Raina

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  1. Behrouz Shiran
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Shiran, B., Kiani, S., Sehgal, D. et al. Internal transcribed spacer sequences of nuclear ribosomal DNA resolving complex taxonomic history in the genus Vicia L.. Genet Resour Crop Evol 61, 909–925 (2014). https://doi.org/10.1007/s10722-014-0086-5

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