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Chemical characterization of aroma compounds in essential oil isolated from “Holy Basil” (Ocimum tenuiflorum L.) grown in India

Genetic Resources and Crop Evolution volume 60, pages 1727–1735 (2013)Cite this article

Abstract

Ocimum tenuiflorum L. (“Holy Basil”/“Tulsi”) is an indigenous Ayurvedic medicinal plant of India, widely distributed in tropical and warm temperate regions. Plant is well known for its enormous therapeutic activities and prevention against diseases. In the present study, thirty-two accessions of O. tenuiflorum germplasm collected from different regions of Northern India were evaluated for their essential oil content and composition. Essential oil obtained by hydrodistillation was analyzed by GC and GC/MS for aroma compounds. Essential oil content in O. tenuiflorum germplasm showed wide range of variation from 0.13 to 0.45 % on FWB. GC and GC/MS profile of O. tenuiflorum oil showed phenylpropanoids, mainly eugenol constituted the major proportion of essential oil. The range of major chemical constituents identified were eugenol (1.94–60.20 %), methyl eugenol (0.87–82.98 %), β-caryophyllene (4.13–44.60 %), β-elemene (0.76–32.41 %). Eugenol and methyl eugenol rich two chemotypes were identified in O. tenuiflorum germplasm. The accessions, IC583281, IC583285 and IC583322 were found to contain high eugenol content (>55 %), while three accessions IC583279, IC583284 and IC583278 were found to possess high methyl eugenol content (>70 %). Eugenol has been reported to be largely responsible for the therapeutic potentials of tulsi, while methyl eugenol is a high value aroma chemical used as flavouring agent. The results suggest holy basil have a potential as a new essential oil crops and can provide oil yields and composition typical for the respective species.

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References

  • Adams RP (2001) Identification of essential oil components by gas chromatography/mass spectrometry, carol stream, 1L. Allured Publishing Corporation, USA

    Google Scholar 

  • Anonymous (1991) Wealth of India, Vol 7. Publication and Information Directorate, CSIR, New Delhi, pp 79–89

  • Davies NW (1990) Gas chromatography retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20 m phases. J Chromatogr 503:1–24

    Article  CAS  Google Scholar 

  • Grayer RJ, Kite GC, Goldstone FJ, Bryan SE, Allan P, Putievsky E (1996) Infraspecific taxonomy and essential oil chemotypes in sweet basil, Ocimum basilicum. Phytochemistry 43:1033–1039

    PubMed  Article  CAS  Google Scholar 

  • Guenther E (1949) The Essential Oils. Van Nostrand, New York, pp 519–520

    Google Scholar 

  • Gupta SK, Prakash J, Srivastava S (2002) Validation of traditional claim of Tulsi, Ocimum sanctum Linn. as a medicinal plant. Indian J Exp Biol 40:765–773

    PubMed  CAS  Google Scholar 

  • Kirtikar KR, Basu BD (1975) Indian Medicinal Plant (2nd ed) Bishen Singh Mahendra Pal Singh, New Connaught Place, Dehradun, India, Vol 3, pp 1965–1968

  • Kothari SK, Bhattacharya AK, Singh CP, Singh K, Syamasundar KV, Ramesh S, Kumar S (2001) A new methyl eugenol-rich and short duration strain of Ocimum tenuiflorum. J Med Arom Pl Sci 22 (4A) & 23(1A):385–388

    Google Scholar 

  • Krüger H, Wetzel SB, Zeiger B (2002) The chemical variability of Ocimum species. J Herbs Spices Med Plants 9(4):335–344

    Article  Google Scholar 

  • Lawrence BM, Hogg JW, Terhune SJ, Pichitakul N (1972) Essential oils and their constituents. IX. The oils of Ocimum sanctum and Ocimum basilicum from Thailand. Flav Industry, pp 47–49

  • Machado MIL, Silva MGV, Matos FJA, Craveiro AA, Alencar JW (1999) Volatile constituents from leaves and inflorescence oil of Ocinum tenuiflorum L. (syn. O. sanctum L.) grown in northeastern Brazil. J Essent Oil Res 11:324–326

    Article  CAS  Google Scholar 

  • Maheshwari ML, Singh BM, Gupta R, Chien MJ (1987) Essential oil of sacred basil (Ocimum sanctum). Indian Perfum 31:137–145

    CAS  Google Scholar 

  • Malik MS, Rafique M, Ahmed R (1986) Essential oils of the species of Labiatae 2. Studies on the essential oil of Ocimum sanctum. Pak J Sci Ind Res 29:111–112

    CAS  Google Scholar 

  • Mondello L, Zappia G, Cotroneo A, Bonaccorsi I, Chowdhury JU, Yusuf M, Dugo G (2002) Studies on the essential oil bearing plants of Bangladesh. Part VIII. Composition of some Ocimum oils O.basilicum L. var. purpurascens; O. sanctum L. green; O. sanctum L. purple; O. americanum L., citral type; O. americanum L., camphor type. Flav Fragr J 17:335–340

    Article  CAS  Google Scholar 

  • Padalia RC, Verma RS (2011) Comparative volatile oil composition of four Ocimum species from northern India. Nat Prod Res 25:569–575

    PubMed  Article  CAS  Google Scholar 

  • Pattanayak P, Behera P, Das D, Panda SK (2010) Ocimum sanctum Linn. A reservoir plant for the therapeutic application: an overview. Phcog Rev 4:95–105

    Article  CAS  Google Scholar 

  • Philip MP, Damodaran NP (1985) Chemotypes of Ocimum sanctum Linn. Indian Perfum 29:49–56

    CAS  Google Scholar 

  • Pingale SS, Firke NP, Markandetya AG (2012) Therapeutic activities of Ocimum tenuiflorum accounted in last decade: a review. J Pharm Res 5:2215–2220

    Google Scholar 

  • Pino JA, Rosado A, Rodriguez M, Garcia D (1998) Composition of the essential oil of Ocimum tenuiflorum L. grown in Cuba. J Essent Oil Res 10:437–438

    Article  CAS  Google Scholar 

  • Pistrick K et al. (2001) Labiatae. In: Hanelt P, Institute of Plant genetics and crop plant research (eds.), Mansfeld’s encyclopedia of agricultural and horticultural crops, vol. 4. Springer, Berlin, pp 1954–1960

  • Prakash P, Gupta N (2005) Therapeutic Uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian J Physiol Pharmacol 49:125–131

    PubMed  CAS  Google Scholar 

  • Pushpangadan P, Bradu BL (1995) Basil. In: Chadha KL, Gupta R (eds.) Advances in Horticulture, Medicinal and Aromatic Plants, Malhotra Pub House, Vol 11, New Delhi, India, pp 628–657

  • Rahman S, Islam R, Kamruzzaman M, Alam K, Jamal AHM (2011) Phytochemical and Pharmacological profile of Ocimum sanctum L. Am J Drug Discov Dev: 1–15. doi: 10.3923/rjmp.2011

  • Vani SR, Cheng SF, Chuah CH (2009) Comparative study of volatile compounds from genus Ocimum. Am J Appl Sci 6:523–528

    Article  CAS  Google Scholar 

  • Zheljazkov VD, Cantrell CL, Evans WB, Ebelhar MW, Coker C (2008) Yield and composition of Ocimum basilicum L. and Ocimum sanctum L. grown at four locations. HortScience 43:737–741

    Google Scholar 

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Acknowledgments

The authors are thankful to Director, NBPGR for providing facilities for conducting the studies.

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  1. Germplasm Evaluation Division, National Bureau of Plant Genetic Resources, New Delhi, 110012, India

    Archana P. Raina, Ashok Kumar & M. Dutta

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  1. Archana P. Raina
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  2. Ashok Kumar
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  3. M. Dutta
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Correspondence to Archana P. Raina.

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Raina, A.P., Kumar, A. & Dutta, M. Chemical characterization of aroma compounds in essential oil isolated from “Holy Basil” (Ocimum tenuiflorum L.) grown in India. Genet Resour Crop Evol 60, 1727–1735 (2013). https://doi.org/10.1007/s10722-013-9981-4

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  • DOI: https://doi.org/10.1007/s10722-013-9981-4

Keywords

  • β-caryophyllene
  • β-elemene
  • Essential oil composition
  • Eugenol
  • Holy Basil
  • Lamiaceae
  • Methyl eugenol
  • Ocimum sanctum L.
  • Ocimum tenuiflorum L.
  • Tulsi