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Genetic Resources and Crop Evolution

, Volume 60, Issue 4, pp 1209–1222 | Cite as

EST-SSR markers reveal synonymies, homonymies and relationships inconsistent with putative pedigrees in chestnut cultivars

  • Tim McCleary
  • Mary McAllister
  • Mark Coggeshall
  • Jeanne Romero-SeversonEmail author
Research Article

Abstract

Over the last two centuries, chestnut breeding programs in Europe and Asia have generated an array of chestnut interspecific hybrids, primarily of European (Castanea sativa), Japanese (C. crenata) and Chinese (C. mollissima) ancestry. During this same period, Europeans colonizing North America imported hybrid chestnuts and made interspecific hybrids with native chestnuts, primarily American chestnut (C. dentata). The importation of Chinese chestnut into the United States in the late 19th century also introduced chestnut blight, which triggered an additional interspecific hybridization effort in an attempt to develop blight resistant American chestnuts. Chestnut cultivars used for nut production in the United States and Canada have arisen against this background of non-native introductions and extensive hybridizing. The development of regionally adapted nut producing trees with dependable crops of high quality nuts requires sorting out the identities of existing cultivars. We chose 11 EST-SSR markers from C. mollissima for the initial task of genotyping 65 chestnut cultivars that grow well in the central United States. Many of these cultivars have interspecific pedigrees involving two or more species. We found extensive homonymies and synonymies, genetic groups inconsistent with published pedigrees, contradictory pedigrees and evidence for incorrect species assignments. Accurate inference of the interspecific ancestries of cultivars grown in the United States and Canada will require genotyping of species reference sets for C. sativa, C. crenata, C. mollissima, C. dentata and possibly C. pumila (the Ozark and Allegheny chinquapins).

Keywords

Chestnut cultivars Castanea EST-SSR Interspecific hybrids 

Notes

Acknowledgments

The authors thank Rory Carmichael (Notre Dame Bioinformatics Core Facility) for assistance with bioinformatics and Brent Harker (Notre Dame Genomics Core Facility) for ABI3730xl services. This work was funded through the University of Missouri Center for Agroforestry under cooperative agreement 58-6227-9-059 with the USDA Agricultural Research Service (ARS). Any opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the United States Department of Agriculture.

Supplementary material

10722_2012_9912_MOESM1_ESM.pdf (576 kb)
Supplementary material 1 (PDF 575 kb)
10722_2012_9912_MOESM2_ESM.docx (49 kb)
Supplementary material 2 (DOCX 48 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Tim McCleary
    • 1
  • Mary McAllister
    • 1
  • Mark Coggeshall
    • 2
  • Jeanne Romero-Severson
    • 1
    Email author
  1. 1.Department of Biological SciencesUniversity of Notre DameNotre DameUSA
  2. 2.Center for AgroforestryUniversity of MissouriColumbiaUSA

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