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Genetic diversity analysis of hulless barley from Shangri-la region revealed by SSR and AFLP markers

Genetic Resources and Crop Evolution volume 59, pages 1543–1552 (2012)Cite this article

Abstract

For adding the hulless barley resources of Shangri-la region to the global barley resource library, a basic work was done by us to assess their genetic diversity of this region. The genetic diversity of 60 hulless barley samples collected from three counties in Shangri-la region of Yunnan Province, were studied using SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphism) markers. A total of 70 alleles were detected for 19 pairs of SSR primers, and 525 band containing 464 polymorphic bands were revealed for 5 pairs of AFLP primers. The value of polymorphism information content (PIC) ranged from 0.03 to 0.86 for SSR primers. The total numbers of alleles were 51, 55, 43 in three populations and the polymorphic bands were 188, 205 and 141. The genetic distances and genetic identity among the three populations showed their close relationship. The gene diversity among populations relative to the total population diversity (Gst) was 0.13 for SSR markers and 0.02 for AFLP markers and indicated that just 13 and 2% variations were among populations, respectively. The UPGMA cluster analysis revealed that all of the samples grouped randomly rather than clustered into distinct groups corresponding to their populations, row types and spring/fall types. We concluded that there was high genetic diversity in the population of Shangri-la region and the formation of diversity was related to complex environment and inhabitants’ traditional practices.

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Acknowledgments

We thank Liu YT, Yang CY, Liu YC, Shi YN (Kunming Institute of Botany, China) for assisting sample collection. This research was supported by the UNEP-GEF (GFL/2328-2715-49), the Ministry of Education of China through its 985 and 111 projects (MUC 98503-001006 & B08044), the Chinese Academy of Sciences (O92441112F & KSCX2-YW-Z-0925), the National Science Foundation of China (31070288), the Japan Society for the Promotion of Science (JSPS/AP/109080), and the National Natural Science Foundation of China (31161140345).

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Authors and Affiliations

  1. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People’s Republic of China

    Yu Guo, Yali Li, Yuan Huang & Chunlin Long

  2. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, People’s Republic of China

    Yu Guo & Lijuan Chen

  3. Chinese Academy of Sciences, Graduate University, Beijing, 100049, People’s Republic of China

    Yali Li

  4. Bioversity International, Via dei Tre Denari 472/a, 00057, Maccarese, Rome, Italy

    Devra Jarvis

  5. Research Institute for Bioresources, Okayama University, Kurashiki 12, 710-0046, Japan

    Kazuhiro Sato

  6. Faculty of Agriculture, Okayama University, Okayama, 700-8530, Japan

    Kenji Kato

  7. College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, People’s Republic of China

    Chunlin Long

  8. Faculty of Bioresource Sciences, Akita Prefectural University, Akita, 010-0444, Japan

    Hiroshi Tsuyuzaki

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  1. Yu Guo
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  2. Yali Li
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  3. Yuan Huang
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  8. Lijuan Chen
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  9. Chunlin Long
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Correspondence to Chunlin Long.

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Guo, Y., Li, Y., Huang, Y. et al. Genetic diversity analysis of hulless barley from Shangri-la region revealed by SSR and AFLP markers. Genet Resour Crop Evol 59, 1543–1552 (2012). https://doi.org/10.1007/s10722-011-9783-5

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  • DOI: https://doi.org/10.1007/s10722-011-9783-5

Keywords

  • Genetic diversity
  • Hordeum vulgare
  • Hulless barley
  • Shangri-la region

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