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Genetic Resources and Crop Evolution

, Volume 59, Issue 5, pp 901–908 | Cite as

ISSR analysis shows low genetic diversity versus high genetic differentiation for giant bamboo, Dendrocalamus giganteus (Poaceae: Bambusoideae), in China populations

  • Bo Tian
  • Han-Qi Yang
  • Khoon-Meng Wong
  • Ai-Zhong Liu
  • Zhen-Yuan Ruan
Research Article

Abstract

Dendrocalamus giganteus Munro is a high-value woody bamboo widely grown in Southeast Asia and China’s Yunnan Province. We investigated its genetic diversity in Yunnan as a prelude to considering effective breeding programs and the protection of germplasm resources. Inter-simple sequence repeat (ISSR) markers were used to assess the genetic structure and differentiation of seven populations. Seven ISSR primers generated 140 bands, of which 124 were polymorphic (88.57%). Genetic diversity within populations was relatively low, averaging 11.33% polymorphic bands (PPB), while diversity was considerably higher among populations, with PPB = 88.57%. Greater genetic differentiation was detected among populations (G ST = 0.8474). We grouped these seven populations into two clusters within an UPGMA dendrogram—one comprised the Xinping and Shiping populations from central Yunnan, the other included the remaining five populations. Mantel tests indicated no significant correlation between genetic and geographic distances among populations. Breeding system characteristics, genetic drift, and limited gene flow (N m = 0.0901) might be important factors for explaining this differentiation. Based on the overall high genetic diversity and differentiation among D. giganteus populations in Yunnan, we suggest the implementation of in situ conservation measures for all populations and sufficient sampling for ex situ conservation collections.

Keywords

Dendrocalamus giganteus Genetic variation ISSR Population structure 

Notes

Acknowledgments

We thank two anonymous reviewers for their constructive comments and suggestions. We are also grateful to Dr. Long-Qian Xiao for his kind help in data analysis. This work was funded through projects of the National Natural Science Foundation of China (31070593), Applied Basic Research Program of Yunnan Province, China (2010CD141), the State Forestry Administration of China (2008-4-30), and International Centre for Bamboo and Rattan (1632009014).

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Bo Tian
    • 1
    • 2
    • 3
  • Han-Qi Yang
    • 3
  • Khoon-Meng Wong
    • 4
  • Ai-Zhong Liu
    • 2
  • Zhen-Yuan Ruan
    • 3
  1. 1.Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunmingChina
  2. 2.Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunmingChina
  3. 3.Research Institute of Resource InsectsChinese Academy of ForestryKunmingChina
  4. 4.Singapore Botanic GardensSingaporeSingapore

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