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Karyotypic studies on Campanumoea (Campanulaceae)—endemic to China

  • Notes on Neglected and Underutilized Crops
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Abstract

Radix Campanumoeae (C. javanica Bl.) has been used in Miao herbal medicine to treat neurasthenia and consumptive disease for hundreds of years. Though Radix Campanumoeae shows great potential for utilization in medical studies, this herb crop has not been cultivated industrially in China. Many species in the Campanumoea genus are similar in phenotype; therefore, a karyotypic study would prove useful for clearly distinguishing Radix Campanumoeae from related species within the same genus, for germplasm preservation and for breeding Radix Campanumoeae. 10 accessions of four species in this genus and 5 accessions in 5 relative genera from SCSB were used for karyotype determination. The results showed a karyotype of 2n = 16 = 2m + 12sm + 1st in Campanumoea, and a karyotype of 2n = 18 = 6 m + 12sm in Cyclocodon. Based on the chromosome number and the karyotypic formula, we suggest that the Campanumoea genus can be divided into two genera, which is in agreement with results observed from pollen morphology and from homological usage in Chinese herbal medicine. The chromosome length in C. javanica subsp. javanica and C. javanica subsp. japonica Makino, ranged from 2.24 to 1.38 μm and 2.04 to 1.31 μm, respectively; and their haploid sets were almost identical (13.15 and 13.16 μm, respectively). This indicates that chromosomal rearrangements occur within chromosomes IV, V and VII without a net gain or loss of genetic material.

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References

  • Arano H, Saito H (1975) Cytological studies in family Campulaceae II. Karyotypes in Adenophora (I). La Kromosomo 99:3072–3081

    Google Scholar 

  • Barbash DA, Awadall P, Tarone AM (2004) Functional divergence caused by ancient positive selection of a Drosophila hybrid incompatibility locus. PLoS Biol 2:839–848

    Article  CAS  Google Scholar 

  • Blanc G, Hokamp K, Wolfe KH (2003) A recent polyploidy superimposed on older large-scale duplications in the Arabidopsis genome. Genome Res 13:137–144

    Article  PubMed  CAS  Google Scholar 

  • Böcher T (1964) Chromosome connections and aberrations in the Campanula persicifolia group. Svensk Bot Tidskr 58:1–18

    Google Scholar 

  • Bowers JE, Chapman BA, Rong J, Paterson AH (2003) Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature 422:433–438

    Article  PubMed  CAS  Google Scholar 

  • Clarke CB (1981) Campanulaceae. In: Hooker JD (ed) The Flora of Britian India, vol 3. Reeve, London

    Google Scholar 

  • Contandriopoulos J (1984) Differentiation and evolution of the genus Campanula in the Mediterranean region. In: Plant Biosystematics. Academic Press, Canada, pp 141–158

  • Dharmananda S (2002) The nature of Ginseng: from traditional use to modern research. Available via DIALOG http://www.itmonline.org/journal/arts/ginsengnature.htm

  • Doebley J, Stec A, Hubbard L (1997) The evolution of apical dominance in maize. Nature 386:485–488

    Article  PubMed  CAS  Google Scholar 

  • Dong X, Braun EL, Grotewold E (2001) Functional conservation of plant secondary metabolic enzymes revealed by complementation of arabidopsis flavonoid mutants with maize genes. Plant Physiol 127:46–57

    Article  PubMed  CAS  Google Scholar 

  • Eddie WMM (1997) A Global reassessment of the generic relationships in the bellflower family (Campanulaceae). Ph. D. Thesis, University of Edinburgh

  • Eddie WMM, Ingrouille MJ (1999) Polymorphism in the Aegean ‘‘five-loculed’’ species of the genus Campanula, sect. Quinqueloculares (Campanulaceae). Nordic J Bot 19:153–169

    Article  Google Scholar 

  • Gadella TWJ (1966) Some notes on the delimitation of genera in the Campanulaceae. Proc Koninkl Nederl Akad Wetensch (ser C) 69:502–521

    Google Scholar 

  • Gompel N, Carroll SB (2003) Genetic mechanisms and constraints governing the evolution of correlated traits in drosophilid flies. Nature 424:931–935

    Article  PubMed  CAS  Google Scholar 

  • Greenberg AJ, Moran JR, Coyne JA et al (2003) Ecological adaptation during incipient speciation revealed by precise gene replacement. Science 302:1754–1757

    Article  PubMed  CAS  Google Scholar 

  • Han GR, He XF, Yang JH et al (1990) Chemica1 study on the root of Codonopsis pilosula. China J Chin Mater Med 15(2):41–42

    Google Scholar 

  • Hong DY (1983) Angiospermae: Dicotyledoneae: Campanulaceae, Goodeniaceae, Stylidiaceae, In: Agendae Academiae Sinicae Edita (eds) Flora Reipublicae Popularis Sinicae. Tomus vol 73, no 2: Beijing, Science Press, pp 69–74

  • Hong DY (1995) The geography of the Campanulaceae: on the distribution centres. Acta Phytotaxon Sin 33:521–536

    Google Scholar 

  • Hong DY, Ma LM (1991) Systematics of the genus Cyananthus Wall. ex Royle. Acta Phytoaxon Sin 29:25–51

    Google Scholar 

  • Hong DY, Pan KY (1998) The restoration of the genus cyclocodon (Campanulaceae) and its evidence from pollen and seed-coat. Acta Phytotaxon Sin 36(2):106–110

    Google Scholar 

  • Jee V, Dhar U, Kachroo P (1989) Cytogeography of some endemic taxa of Kashmir Himalaya. Proc Indian Natl Sci Acad (part B) 55:177–184

    Google Scholar 

  • Johanson U, West J, Lister C et al (2000) Molecular analysis of FRIGIDA, a major determinant of natural variation in Arabidopsis flowering time. Science 290:344–347

    Article  PubMed  CAS  Google Scholar 

  • Ke-Qing W, Song GE (1998) A karyotype study on five species of adenophora. Acta Bot Yunnanica 20(1):58–62

    Google Scholar 

  • Khoshbakht K, Hammer K, Pistrick K (2007) Eryngium caucasium Trautv. Cultivated as a vegetable in the Elburz Mountains (Northern Iran). Genet Resour Crop Evol 54:445–448

    Article  Google Scholar 

  • Krasnoborov IM, Rostovtseva TS, Ligus SA (1980) Chromosome numbers of some plant species of South Siberia and the Far East. Bot Zurn 65:659–668

    Google Scholar 

  • Kroymann J, Donnerhake S, Schnabelrauch D et al (2003) Evolutionary dynamics of an Arabidopsis insect resistance quantitative trait locus. Proc Natl Acad Sci USA 100(Suppl. 2):14587–14592

    Article  PubMed  CAS  Google Scholar 

  • Kumar V, Chauhan KPS (1975) Cytology of Cyananthus linifolius Wall. Cat Proc Indian Sci Congr Assoc 62, Group B: 130

  • Lammers TG (1992) Systematics and biogeography of the Campanulaceae of Taiwan. In: Peng C-I (ed), Phytogeography and botanical inventory of Taiwan. Inst Bot Acad Sin Monogr Ser No 12, Taipei, pp 41–61

  • Lee S, Chung YJ, Lee J (1988) A palynotaxonomic study of the Korean Campanulaceae. Korean J Plant Taxon 18:115–131

    Google Scholar 

  • Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220

    Article  Google Scholar 

  • Li ming, Tian Su ying, Xu wan ting (2008) Primary study on pharmacognostics of Tu Dang Shen (C. javanica) native shui yuan Mountain in Guang dong. J Chin med mater 31(4):511–513

    Google Scholar 

  • Ling you run (1995) A new compendium of materia medica: pharmaceutical botany and chinese medicinal plants. Science Press, Beijing

  • Manda’kova’ T, Lysak MA (2008) Chromosomal phylogeny and karyotype evolution in x = 7 crucifer species (Brassicaceae). Plant Cell 20:2559–2570

    Article  Google Scholar 

  • Moeliono B, Tuyn P (1960) Campanulaceae. In: van Steenis CGGJ (ed) Flora Malesiana, Ser. 1, voe. 6(1). Noordhoff-Kolff, Djakarta, pp 107–141

    Google Scholar 

  • Morris KE, Lammers TG (1997) Circumscription of Codonopsis and the allied genera Campanumoea and Leptocodon (Campanulaceae: Campanuloideae). I. Palynolodical data. Bot Bull Acad Sin 38:277–284

    Google Scholar 

  • Murthy GVS (1983) Pollen morphology of Indian Campanumoe Bl.-a revision of the Genus. J Palynol 18:55–59

    Google Scholar 

  • Nishikawa T (1985) Chromosome counts of flowering plants of Hokkaido (8). J Hokkaido Univ Educ (sect. 2B) 35:97–111

    Google Scholar 

  • Otto SP, Whitton J (2000) Polyploid incidence and evolution. Annu Rev Genet 34:401–437

    Article  PubMed  CAS  Google Scholar 

  • Qiu JZ, Hong DY (1987) A cytogeographical study on Adenophora gmelinii complex (Campanulaceae). In: Hong (ed) Plant chromosome research.Proc Sino—Jpn Symposium P1 Chromos Hiroshima, Nishiki Print Co, pp 69–73

  • Raven PH (1975) The bases of angiosperm phylogeny: cytology. Ann Missouri Bot Gard 62:724–764

    Article  Google Scholar 

  • Rosén W (1931) Zur Embryologie der Campanulaceen und Lobeliaceen. Acta Horti Gothob 7:31–42

    Google Scholar 

  • Shimizu T (1993) Campanulaceae. In: Iwatsuki K et al (eds) Flora of Japan, vol 3a. Kodansha, Tokyo, pp 405–418

    Google Scholar 

  • Stebbins GL (1971) Chromosomal evolution in higher plants. Addison-Wesley Publishing Company, London

    Google Scholar 

  • Sui WB, Cui QH, Jia YF (1985) Karyotype analysis of Carthamus tinotorius L. and Codonopsis pilosula Nannf. J Wuhan Bot Res 3:459–460

    Google Scholar 

  • Tian YS, Zhao XM (2007) The karyotype analysis of chromosomes of Codonopsis pilosula (Franch.) Nannf. Chin Wild Plant Resour 2:105–110

    Google Scholar 

  • Tsoong PC (1935) Preliminary study on Chinese Campanulaceae. Contr Inst Bot Natl Acad Peiping 3:61–118

    Google Scholar 

  • Van Thuan N (1969) Campanulaceae. In: Aubréville A (ed) Flora du Cambodge, du Laos et du Vietman, fasc. 9. Muésum National d’Histoire Naturelle, Paris

  • Vision TJ, Brown DG, Tanksley SD (2000) The origins of genomic duplications in Arabidopsis. Science 290:2114–2117

    Article  PubMed  CAS  Google Scholar 

  • Wang Y (2002) The chromatic book of Chinese medication of MIAO. Guizhou Scientific and Technical Press, Guiyang

    Google Scholar 

  • Wendel J, Doyle J (2005) Polyploidy and evolution in plants. In: Henry RJ (ed) Plant diversity and evolution: genotypic and phenotypic variation in higher plants. CABI, Oxfordshire (UK), pp 97–117

    Chapter  Google Scholar 

  • Wolfe KH (2001) Yesterday’s polyploidization and the mystery of diploidization. Nat Rev Genet 2:333–341

    Article  PubMed  CAS  Google Scholar 

  • Yang xiao sheng, Zhu wen shi, Hao xiao jiang (2005) The study of chemical component of Tu dang shen (C. javanica). Chin Tradit Herb Drugs 36(8):1144–1146

    Google Scholar 

  • Yoo KO, Lee WT (1989) A taxonomic study of the genus Codonopsis in Korea. Korean J Plant Taxon 19:81–102

    Google Scholar 

  • Yu J, Wang J, Lin W, Li S, Li H et al (2005) The genomes of Oryza sativa: a history of duplications. PLoS Biol 3:e38

    Article  PubMed  Google Scholar 

  • Zhang Y-F, Zhang C-Y, Zhang T et al (2010) A cyto-evolutional study of Campanumoea Blume (Campanulaceae) and a possible pathway for secondary karyotype formation. Plant Syst Evol 285:245–257

    Article  Google Scholar 

  • Zhao GT, Li M (2005) The nucleus type analysis of the chromosome of the Codonopsis lanceolata. Forest By-Prod Special Chin 1:37–41

    Google Scholar 

Download references

Acknowledgments

We thank the Chinese South-West Wild Germplasm Source Bank for material. Support by national natural science foundation of China (30660075, 40861019, 31060150) and Yunnan Science and Technology Social Development Projection (2009CD052) is gratefully acknowledged.

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  1. Chun-Yong Zhang

    Present address: Meng Zhi Wenlan High Middle School, North Street of South Lake, 661100, MengZhi, Yunnan, China

Authors and Affiliations

  1. School of Biological Science, Yunnan Normal University, 121 Street, 650092, Kunming, China

    Yun-Feng Zhang & Chun-Yong Zhang

  2. Kunming Institute of Botany, Chinese Academic of Science, 650204, Kunming, China

    Ting Zhang

  3. Sustainable Development and Utilization of Bio-Energy Engineering Research Center, Ministry of Education, 650092, Kunming, China

    Yun-Feng Zhang

  4. The College of Energy Sources and Environment, Yunnan Normal University, 650092, Kunming, China

    Hui-Ling Guang

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  1. Yun-Feng Zhang
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Correspondence to Yun-Feng Zhang.

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Zhang, YF., Zhang, CY., Zhang, T. et al. Karyotypic studies on Campanumoea (Campanulaceae)—endemic to China. Genet Resour Crop Evol 58, 461–470 (2011). https://doi.org/10.1007/s10722-010-9655-4

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