Abstract
Using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), the different alleles encoded at the 6 glutenin loci and 3 ω-gliadin loci were identified from a set of 134 hexaploid and 128 tetraploid wheat accessions mainly grown in Portugal. In the hexaploid wheats (T. aestivum L.), a total of 56, 42 and 36 patterns were observed for high molecular weight-glutenin subunits (HMW-GS), low molecular weight-glutenin subunits (LMW-GS) and ω-gliadins respectively. For HMW-GS encoded at Glu-A1, Glu-B1 and Glu-D1 loci, 4, 10 and 6 alleles were observed, respectively. LMW-GS displayed similar polymorphism, as Glu-A3, Glu-B3 and Glu-D3 loci, which comprises 5, 9 and 3 alleles. Twenty-four alleles were observed for ω-gliadins found at Gli-A1, Gli-B1 and Gli-D1 loci with, 5, 16 and 3 alleles respectively. For tetraploid collection fifty different alleles were identified for the seven loci studied Glu-A1 (3), Glu-B1 (13), Glu-A3 (6), Glu-B3 (7), Glu-B2 (2), Gli-A1 (5) and Gli-B1 (14). The genetic distances within hexaploid and tetraploid wheats were presented using cluster representation. The mean value of genetic variation indices (H) for wheat storage protein loci was slightly lower in current commercially available varieties (0.592) and highest for old varieties (0.574).
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Acknowledgments
We gratefully acknowledge Dr. Sadequr Rahman from CSIRO, Plant Industry for his help in revising the English in this paper. We are also grateful to Benvindo Maçãs, from the Instituto Nacional de Investigação Agrária e das Pescas (INIAP), Elvas, Portugal who kindly provided a number of seeds of the Old Collection. The authors thank the Direcção-Geral de Agricultura e Desenvolvimento Rural, Direcção de Serviços de Fitossanidade e de Materiais de Multiplicação de Plantas, Divisão de Sementes, Variedades e Recursos Genéticos for kindly donating the grains of commercial varieties.
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Ribeiro, M., Carvalho, C., Carnide, V. et al. Towards allelic diversity in the storage proteins of old and currently growing tetraploid and hexaploid wheats in Portugal. Genet Resour Crop Evol 58, 1051–1073 (2011). https://doi.org/10.1007/s10722-010-9642-9
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DOI: https://doi.org/10.1007/s10722-010-9642-9