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Genetic relationships among local potato cultivars from Spain using SSR markers

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Abstract

Microsatellite markers (SSR) were used to fingerprint a total of 105 local potato cultivars from Spain. A set of 41 cultivars from Tenerife Island, 19 from the island of La Palma, and 45 local varieties from peninsular Spain were analysed. Some of these varieties represent relicts of the early introductions originating from South America and have been characterised previously morphologically and ecophysiologically. We observed within our materials a total of 76 SSR alleles. Only seven of them were present in all varieties. Several accession and group specific alleles were detected. Similarity coefficients were computed from the molecular data and cluster analyses were performed. The obtained dendrogram was generally in good agreement with previous classifications of the accessions as Solanum tuberosum L. subsp. andigena (Juz. et Bukasov) Hawkes S. tuberosum L. subsp. tuberosum and S. chaucha Juz. et Bukasov genotypes. Also cultivar groups with identical or related common names showed the same SSR patterns or clustered closely together. In addition we performed Principal Coordinate Analysis with the set of genotypes. Results of both analysis methods were generally in good agreement, but also some smaller differences were detected in the associations of groups and genotypes. According to the molecular patterns for some accessions misleading or confounded names were evident, and in some cases the molecular patterns showed also discrepancies with previous species assignments, suggesting the need for a more detailed comparative study of these accessions.

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Acknowledgments

Part of this work was financed by INIA project RTA2008-00045-C02-01/02, by the Basque Government and by the Cabildo of Tenerife.

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Authors and Affiliations

  1. NEIKER-Tecnalia. Instituto Vasco de Investigación y Desarrollo Agrario, Apdo. 46, 01080, Vitoria, Spain

    Jose Ignacio Ruiz de Galarreta, Leire Barandalla, Raquel Lopez & Enrique Ritter

  2. Centro de Conservación de la Biodiversidad Agrícola de Tenerife, Cabildo Insular de Tenerife, 38007, Tacoronte (Tenerife), Spain

    Domingo José Rios

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  1. Jose Ignacio Ruiz de Galarreta
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  2. Leire Barandalla
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  3. Domingo José Rios
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Correspondence to Jose Ignacio Ruiz de Galarreta.

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de Galarreta, J.I.R., Barandalla, L., Rios, D.J. et al. Genetic relationships among local potato cultivars from Spain using SSR markers. Genet Resour Crop Evol 58, 383–395 (2011). https://doi.org/10.1007/s10722-010-9583-3

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