Abstract
A novel dwarf mutant of Brassica napus L. em. Metzg., named NDF-1, was derived from a high doubled haploid line ‘3529’ of which seeds were jointly treated with chemical inducers and fast neutron bombardment. The germination results showed that the germination of NDF-1 was insensitive in response to exogenous gibberellic acid 3 (GA3). The studies on growth response to exogenous GA3 showed that NDF-1 seeding has at least 10-fold insensitivity than the wild-type. Moreover, no matter what concentrations of GA3 were added to the seedlings and adult plants, the NDF-1 could not restore the wild type phenotype. These results indicated that the B. napus dwarf mutant NDF-1 was GA-insensitive mutant. The histological observations showed that the key reason of leading NDF-1 to dwarf was the reduction of hypocotyls and stems cell numbers.
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Acknowledgments
This work was supported by the National ‘863’ Programme (SN: 2001AA241104) and the 10th ‘five-year’ key task project in crop breeding of Sichuan Province (SN: 200107001).
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Gao, Y., Li, T., Zhao, Y. et al. Characterization of the gibberellic acid response of the Brassica napus L. em. Metzg. dwarf mutant NDF-1 . Genet Resour Crop Evol 57, 481–485 (2010). https://doi.org/10.1007/s10722-010-9541-0
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DOI: https://doi.org/10.1007/s10722-010-9541-0