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Genetic diversity associated with conservation of endangered Dongxiang wild rice (Oryza rufipogon)

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Abstract

The wild progenitor species (Oryza rufipogon) of Asian cultivated rice (O. sativa L.) is located in Dongxiang county, China which is considered its the northernmost range worldwide. Nine ex situ and three in situ populations of the Dongxiang wild rice (DXWR) and four groups of modern cultivars were genotyped using 21 SSR markers for study of population structure, conservation efficiency and genetic relationship. We demonstrated that the ex situ conservation of the DXWR failed to maintain the genetic identity and reduced genetic diversity. Therefore, in situ conservation is absolutely necessary to maintain the genetic identity, diversity and heterozygosity. Also, in situ conservation is urgently needed because natural populations in DXWR have decreased from nine to three at present due to farming activity and urban expansion. In DXWR, the three surviving in situ populations had greater expected heterozygosity than any cultivated rice, and were genetically closer to japonica than either the male-sterile maintainer or restorer lines, or indica. Japonica has the lowest genetic diversity of cultivated rice. As a result, DXWR is a rich gene pool and is especially valuable for genetic improvement of japonica rice because these O. rufipogon accessions are most closely related to the japonica as compared to O. rufipogon collected anywhere else in the world.

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Acknowledgments

This work was supported by National Natural Sciences Foundation of China (30960189), Jiangxi Province project for principle research leader (020007), Jiangxi Province Inviting Tender Project for principle research topic (20068) and Application of Nuclear Techniques in Agriculture, Chinese Ministry of Agriculture (Grand number: 200803034).

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Authors and Affiliations

  1. Biotechnology Research Institute, Jiangxi Academy of Agricultural Sciences, 330200, Nanchang, Jaingxi, China

    J. Xie, D. Kong, B. Hu & Y. Wan

  2. College of Life Science, Jiangxi Normal University, 330027, Nanchang, Jaingxi, China

    J. Xie & B. Hu

  3. Rice Research and Extension Center, University of Arkansas, 2900 Hwy 130 East, Stuttgart, AR, 72160, USA

    H. A. Agrama & B. Hu

  4. China National Rice Research Institute, 31006, Hangzhou, Zhejiang, China

    J. Zhuang

  5. USDA-ARS, Dale Bumpers National Rice Research Center, 2890 Hwy 130 East, Stuttgart, AR, 72160, USA

    W. Yan

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  1. J. Xie
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Correspondence to J. Xie.

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Xie, J., Agrama, H.A., Kong, D. et al. Genetic diversity associated with conservation of endangered Dongxiang wild rice (Oryza rufipogon). Genet Resour Crop Evol 57, 597–609 (2010). https://doi.org/10.1007/s10722-009-9498-z

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  • DOI: https://doi.org/10.1007/s10722-009-9498-z

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