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Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species

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Abstract

The primary structure of the double-headed Bowman-Birk inhibitor (BBI) family and the antitryptic activity were investigated in cultivated and wild Phaseolus species. Two BBI types were identified; the first one inhibits trypsin and chymotrypsin (tc-BBI), the second one elastase and trypsin (et-BBI). Only tc-BBI was found in P. lunatus and P. parvulus, while none of BBI types, identified in this study, was found in P. leptostachyus. The deduced amino acid sequences revealed some polymorphisms within both tc-BBI and et-BBI binding loops that could affect the inhibitory activity. The trypsin inhibitor content showed a high variation with the lowest value recorded in P. lepthostachyus and the highest one observed in P. oligospermus. Southern blot analysis confirmed the absence of both BBI types in P. leptostachyus and suggests that in P. coccineus, P. dumosus and P. costaricensis, the two genes were clustered in a narrow genomic region of 1.3 kbp.

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Acknowledgments

Research partially supported by Ministry of Agriculture Food and Forestry Policies with funds released by C.I.P.E (Resolution 17/2003).

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Authors and Affiliations

  1. Istituto di Genetica Vegetale, CNR, via Amendola 165/A, 70126, Bari, Italy

    Lucia Lioi & Angela Rosa Piergiovanni

  2. Istituto di Biologia e Biotecnologia Agraria, CNR, via Bassini 15, 20133, Milan, Italy

    Incoronata Galasso & Maria Gloria Daminati

Authors
  1. Lucia Lioi
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  2. Incoronata Galasso
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  3. Maria Gloria Daminati
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  4. Angela Rosa Piergiovanni
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Correspondence to Lucia Lioi.

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Lioi, L., Galasso, I., Daminati, M.G. et al. Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species. Genet Resour Crop Evol 57, 533–542 (2010). https://doi.org/10.1007/s10722-009-9491-6

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