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Molecular cytogenetic characterization of the amphiploid between bread wheat and Psathyrostachys huashanica

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Abstract

A new wheat-Psathyrostachys huashanica amphiploid, PHW-SA, was characterized using molecular cytological tools to evaluate the potential utilization of P. huashanica for wheat improvement. PHW-SA pollen mother cells (PMCs) regularly revealed averagely 0.57 univalents, 24.51 ring bivalents, 3.19 rod bivalents and 0.01 trivalents per cell. Complete homologous chromosome pairing was seen in 81% of the PMCs, indicating a degree of cytological stability. P. huashanica C-banding karyotypes of PHW-SA displayed strong heterochromatin terminal bands at either or both ends. 24 P. huashanica chromosome arms showed telomeric bands. Using P. huashanica DNA as a probe and J-11 DNA as blocker, distinctive P. huashanica chromosomes, which were presented entirely greenish-yellow hybridization signals, were observed in a somatic metaphase of PHW-SA. GISH also revealed the chromosomal breakage and translocation occurring in the amphiploid PHW-SA, which may represent a centromeric fusion between P. huashanica and wheat chromosomes. Seeds storage proteins electrophoresis indicated that PHW-SA expressed some of P. huashanica specific gliadin and glutenin bands, and a few gliadin and glutenin bands of the parents disappeared and new bands appeared. The results indicated that the amphiploid PHW-SA could serve as novel germplasm sources for wheat improvement.

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Acknowledgments

The authors are thankful to the National Natural Science Foundation of China (Nos. 30670150, 30870154), and the Education Bureau and Science and Technology Bureau of Sichuan Province, China for the financial supports.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang, Chengdu, Sichuan, China

    Hou-Yang Kang, Qian Chen, Yi Wang, Mei-Yu Zhong, Hai-Qin Zhang & Yong-Hong Zhou

  2. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, 625014, Yaan, Sichuan, China

    Hou-Yang Kang, Hai-Qin Zhang & Yong-Hong Zhou

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  1. Hou-Yang Kang
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  2. Qian Chen
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  3. Yi Wang
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  4. Mei-Yu Zhong
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Correspondence to Yong-Hong Zhou.

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Kang, HY., Chen, Q., Wang, Y. et al. Molecular cytogenetic characterization of the amphiploid between bread wheat and Psathyrostachys huashanica . Genet Resour Crop Evol 57, 111–118 (2010). https://doi.org/10.1007/s10722-009-9455-x

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