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Relationships in the Diplotaxis–Erucastrum–Brassica complex (Brassicaceae) evaluated from isoenzymatic profiles of the accessions as a whole. Applications for characterisation of phytogenetic resources preserved ex situ

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Abstract

Electrophoresis has become one of the most usual tools for plant studies. However, most analyses, using isoenzymes, have been carried out from a population geneticist’s point of view for the study of allelic frequencies. In present work, isoenzyme electrophoresis of bulked seeds was used as a rapid strategy for obtaining the electrophoretic profiles of accessions of a seed bank. The relationships amongst 30 taxa of the complex Diplotaxis-Erucastrum-Brassica (Brassicaceae family, tribe Brassicinae) were analysed, with the electrophoretic profiles of six isoenzyme systems. Each sample presented a stable and characteristic isoenzymatic pattern representative of the whole population. The enzyme markers provided 147 reliable bands which were scored as multistate traits (not with allelic value). UPGMA clustering method showed a dendrogram with three main groups and subgroups of samples that are in agreement with the recognised taxonomy of the taxa. This approach can be a rapid and efficient method to establish the specific enzyme patterns of conserved species populations of a germplasm bank, and the information could be included in the a database as part of documentation system.

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References

  • Al-Shehbaz IA (1985) The genera of Brassiceae (Cruciferae: Brassicaceae) in the southeastern United States. J Arnold Arbor 66:279–351

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3

    Article  CAS  PubMed  Google Scholar 

  • Brewer G, Sing CF (1970) An introduction to isozyme techniques. Academic Press, New York

    Google Scholar 

  • Clemente M, Hernández-Bermejo JE (1980) Clasificación jerárquica de las Brassiceas según caracteres de las piezas estériles de su flor. Jardin Bot Madr 36:97–113

    Google Scholar 

  • Eschmann-Grupe G, Hurka H, Neuffer B (2003) Species relationships within Diplotaxis (Brassicaceae) and the phylogenetic origin of D. muralis. Plant Syst Evol 243:13–29. doi:10.1007/s00606-003-0047-5

    Article  Google Scholar 

  • Gillespie JH, Langley CH (1974) A general model to account for enzyme variation in natural populations. Genetics 76:77–96

    Google Scholar 

  • Gómez-Campo C (1980) Morphology and morphotaxonomy of the Tribe Brassiceae. In: Tsunoda S, Hinata K, Gómez-Campo C (eds) Brassica crops and wild allies. Japan Scientific Societies Press, Tokyo, pp 3–31

    Google Scholar 

  • Gómez-Campo C (1981) Studies on Cruciferae: VIII. Nomenclatural adjustments in Diplotaxis DC. Jardin Bot Madr 38(1):29–35

    Google Scholar 

  • Gómez-Campo C (1982) Studies on Cruciferae: IX. Erucastrum rifanum (Emb. and Maire) Gómez-Campo, comb. nov. Jardin Bot Madr 38:352–356

    Google Scholar 

  • Gómez-Campo C (1983) Studies on Cruciferae: X. Concerning some West Mediterranean species of Erucastrum. Jardin Bot Madr 40:63–72

    Google Scholar 

  • Gómez-Campo C (1984) Studies on Cruciferae: XI. Erucastrum ifniense Gómez-Campo sp. nov. and its allies. Jardin Bot Madr 41:83–85

    Google Scholar 

  • Gómez-Campo C (1993a) Brassica L. In: Talavera S, Aedo C, Castroviejo S, Herrero C, Romero Zarco A, Salgueiro FJ, Velayos M (eds) Flora Iberica. Plantas Vasculares de la Península Ibérica e Islas Baleares, vol 4. Real Jardín Botánico- CSIC (Pub.), Madrid, pp 362–384

    Google Scholar 

  • Gómez-Campo C (1993b) Hirschfeldia Moench. In: Talavera S, Aedo C, Castroviejo S, Herrero C, Romero Zarco A, Salgueiro FJ, Velayos M (eds) Flora Iberica. Plantas Vasculares de la Península Ibérica e Islas Baleares, vol 4. Real Jardín Botánico- CSIC (Pub.), Madrid, pp 398–400

    Google Scholar 

  • Gómez-Campo C (1999) Taxonomy. In: Gómez-Campo C (ed) Biology of Brassica Coenospecies. Elsevier, Amsterdam, pp 3–32

    Chapter  Google Scholar 

  • Gómez-Campo C (2007) Monographs ETSIA, Univ. Politecnica Madr 170:1–17

    Google Scholar 

  • Gómez-Campo C, Martínez-Laborde JB (1998) Reajustes taxonómicos y nomenclaturales en la tribu Brassiceae (Cruciferae). Jardin Bot Madr 52(2):379–381

    Google Scholar 

  • Gómez-Campo C, Tortosa ME (1974) The taxonomic and evolutionary significance of some juvenile characters in the Brassiceae. Bot J Linn Soc 69:105–124. doi:10.1111/j.1095-8339.1974.tb01619.x

    Article  Google Scholar 

  • Harberd DJ (1972) A contribution to the cyto-taxonomy of Brassica (Cruciferae) and its allies. Bot J Linn Soc 65:1–23. doi:10.1111/j.1095-8339.1972.tb00920.x

    Article  Google Scholar 

  • Harberd DJ (1976) Cytotaxonomic studies of Brassica and related genera. In: Vaughan JG, MacLeod AJ, Jones BMG (eds) The biology and chemistry of the Cruciferae. Academic Press, London, pp 47–68

    Google Scholar 

  • Harberd DJ, McArthur ED (1972) The chromosome constitution of Diplotaxis muralis (L.) DC. Watsonia 9:131–135

    Google Scholar 

  • Hedge IC (1976) A systematic and geographical survey of the old world Cruciferae. In: Vaughan JG, MacLeod AJ, Jones BMG (eds) The biology and chemistry of the Cruciferae. Academic Press, London, pp 1–45

    Google Scholar 

  • Heywood VH (1964) Brassica L. In: Tutin TG, Heywood VH, Burges NA, Valentine DH (eds) Flora Europaea, vol 1. Cambridge University Press, Cambridge, pp 335–339

    Google Scholar 

  • Malik M, Vyas P, Rangaswamy NS, Shivanna KR (1999) Development of two new cytoplasmic male-sterile lines in Brassica juncea through wide hybridization. Plant Breed 118:75–78. doi:10.1046/j.1439-0523.1999.118001075.x

    Article  Google Scholar 

  • Martín JP, Sánchez-Yélamo MD (2000) Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers. Theor Appl Genet 101:1234–1241. doi:10.1007/s001220051602

    Article  Google Scholar 

  • Martínez-Laborde JB (1991) Notes on the taxonomy of Diplotaxis DC. (Brassiceae). Bot J Linn Soc 106:67–71. doi:10.1111/j.1095-8339.1991.tb02283.x

    Article  Google Scholar 

  • Martínez-Laborde JB (1993) Diplotaxis. In: Talavera S, Aedo C, Castroviejo S, Herrero C, Romero Zarco A, Salgueiro FJ, Velayos M (eds) Flora Iberica. Plantas Vasculares de la Península Ibérica e Islas Baleares, vol 4. Real Jardín Botánico- CSIC (Pub.), Madrid, pp 346–362

    Google Scholar 

  • Maxted N, Ford-Lloyd BV, Hawkes JG (1997) Complementary conservation strategies. In: Maxted N, Ford-Lloyd BV, Hawkes JG (eds) Plant genetic conservation. Chapman and Hall, London, pp 15–39

    Google Scholar 

  • Mummenhoff K, Eschmann-Grupe G, Zunk K (1993) Subunit polypeptide composition of Rubisco indicates Diplotaxis viminea as maternal parent species of amphiploid Diplotaxis muralis. Phytochemistry 34:429–431

    Article  CAS  Google Scholar 

  • Pradhan AK, Prakash S, Mukhopadhyay A, Pental D (1992) Phylogeny of Brassica and allied genera based on variation in chloroplast and mitochondrial DNA patterns: molecular and taxonomic classifications are incongruous. Theor Appl Genet 85:331–340

    Article  Google Scholar 

  • Rao GU, Batra-Sarup V, Prakash S, Shivanna KR (1994) Development of a new cytoplasmic male sterile system in Brassica juncea through wide hybridization. Plant Breed 112:171–174

    Article  Google Scholar 

  • Rohlf FJ (1992) NTSYS-PC: numerical taxonomy and multivariate analysis system. Version 1.60. Exeter Software, New York

    Google Scholar 

  • Salmeen O (1979) A systematic revision of the genus Brassica L. in the Mediterranean region. PhD. Thesis, Univ. of Reading (Unpub)

  • Sánchez-Yélamo MD (1992) Isoenzyme electrophoretic studies among some species of the genus Erucastrum and Hirschfeldia incana (Cruciferae:Brassiceae) with reference to their chemotaxonomic relationships. Biochem Syst Ecol 20:631–637. doi:10.1016/0305-1978(92)90020-E

    Article  Google Scholar 

  • Sánchez-Yélamo MD (1994) A chemosystematic survey of flavonoids in the Brassicinae: Diplotaxis. Bot J Linn Soc 115:9–18. doi:10.1006/bojl.1994.1027

    Article  Google Scholar 

  • Sánchez-Yélamo MD (2002) Comparative analysis of phenolic compounds among some species of the genus Brassica from Sect. Sinapistrum and Sect. Micropodium. Eucarpia Cruciferae Newsl 24:19–20

    Google Scholar 

  • Sánchez-Yélamo MD (2004) Taxonomic relationships among Erucastrum and Brassica species based on flavonoids compounds. Eucarpia Cruciferae Newsl 25:13–14

    Google Scholar 

  • Sánchez-Yélamo MD (2006) Rapid characterisation of some species of the genus Onobrychis Miller (Leguminosae: Papilionoideae) by isozyme methods. Seed Sci Tech 34:297–306

    Google Scholar 

  • Sánchez-Yélamo M, Ortiz JM, Gogorcena Y (1992) Comparative electrophoretic studies of seed proteins in some species of the genera Diplotaxis, Erucastrum and Brassica. Taxon 41:477–483. doi:10.2307/1222818

    Article  Google Scholar 

  • Schwennesen J, Mielke EA, Wolfe WH (1982) Identification of seedless table grape cultivar and bud sport with berry isozymes. HortScience 17:366–368

    CAS  Google Scholar 

  • Sneath PHA, Sokal RR (1973) The principles and practice of numerical classification. W.H. Freeman, San Francisco

    Google Scholar 

  • Takahata Y, Hinata K (1983) Studies on cytodemes in subtribe Brassicinae (Cruciferae). Tohoku J Agric Res 33:111–124

    Google Scholar 

  • Takahata Y, Hinata KH (1986) A consideration of the species relationships in subtribe Brassicinae (Cruciferae) in view of cluster analysis of morphological characters. Plant Species Biol 1:79–88. doi:10.1111/j.1442-1984.1986.tb00017.x

    Article  Google Scholar 

  • Tsunoda S (1980) Eco-Physiology of wild and cultivated forms in Brassica and allied genera. In: Tsunoda S, Hinata K, Gómez-Campo C (eds) Brassica crops and wild allies. Japan Scientific Societies Press, Tokyo, pp 109–120

    Google Scholar 

  • Warwick SI, Black LD (1993) Molecular relationships in subtribe Brassicinae (Cruciferae, tribe Brassiceae). Can J Bot 71:906–918

    CAS  Google Scholar 

  • Warwick SI, Black LD, Aguinagalde I (1992) Molecular systematics of Brassica and allied genera (Subtribe Brassicinae, Brassiceae) – chloroplast DNA variation in the genus Diplotaxis. Theor Appl Genet 83:839–850. doi:10.1007/BF00226706

    Article  Google Scholar 

Download references

Acknowledgements

I would like to thank Professor Gómez-Campo for suggesting this study and for his continued encouragement and two anonymous reviewers, whose suggestions greatly helped improve the original manuscript. This work was partially supported by grants from the Spanish Ministry of Education and Science (CICYT- 2477).

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  1. Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain

    M. D. Sánchez-Yélamo

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Correspondence to M. D. Sánchez-Yélamo.

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Sánchez-Yélamo, M.D. Relationships in the Diplotaxis–Erucastrum–Brassica complex (Brassicaceae) evaluated from isoenzymatic profiles of the accessions as a whole. Applications for characterisation of phytogenetic resources preserved ex situ . Genet Resour Crop Evol 56, 1023–1036 (2009). https://doi.org/10.1007/s10722-009-9423-5

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