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Genetic diversity of HMW glutenin subunits in diploid, tetraploid and hexaploid Triticum species

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Abstract

The genetic variations of high-molecular-weight (HMW) glutenin subunits in 1051 accessions of 13 Triticum subspecies were investigated using sodium dodecyl sulfate polyacrylamide-gel electrophoresis. A total of 37 alleles were detected, resulting in 117 different allele combinations, among which 20, 68 and 29 combinations were observed in diploid, tetraploid and hexaploid wheats, respectively. Abundance and frequency of allele and combinations in tetraploid wheats were higher than these in hexaploid wheats. Allele Glu-A1c was the most frequent subunit at Glu-A1 locus in tetraploid and hexaploid wheats. Consequently, the results also suggested that the higher variations occurred at Glu-B1 locus compared to Glu-A1 and Glu-D1. Therefore, carthlicum wheat possessing the allele 1Ay could be presumed a special evolutional approach distinguished from other tetraploid species. Furthermore, this provides a convenient approach of induction of the 1Ay to common wheat through direct cross with carthlicum wheat. Alleles Glu-B1c and Glu-B1i generally absent in tetraploid wheats were also found in tetraploid wheats. Our results implied that tetraploid and hexaploid wheats were distinguished in dendrogram, whereas carthlicum and spelta wheats and however displayed the unique performance. In addition, founder effect, no-randomness of diploidization, mutation and artificial selection could cause allele distribution of HMW-GS in Triticum. All alleles of HMW-GS in Triticum could be further utilized through hybrid in the quality improvement of common wheat.

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References

  • An XL, Li QY, Yan YM, Xiao YH, Hsam SLK, Zeller FJ (2005) Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell. ) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci. Euphytica 146:193–201. doi:10.1007/s10681-005-9002-6

    Article  CAS  Google Scholar 

  • Bahraei S, Saidi A, Alizadeh D (2004) High molecular weight glutenin subunits of current bread wheats grown in Iran. Euphytica 137:173–179. doi:10.1023/B:EUPH.0000041546.46063.b5

    Article  CAS  Google Scholar 

  • Barro F, Barcel ÓP, Lazzeri PA, Shewry PR, Ballesteros J, Martín A (2003) Functional properties of flours from field grown transgenic wheat lines expressing the HMW glutenin subunit 1AX1 and 1DX5 genes. Mol Breed 12:223–229. doi:10.1023/A:1026367214120

    Article  CAS  Google Scholar 

  • Blatter RHE, Jacomet S, Schlumbaum A (2002) Spelt-specific alleles in HMW glutenin genes from modern and historical European spelt (Triticum spelta L.). Theor Appl Genet 104:329–337. doi:10.1007/s001220100680

    Article  PubMed  CAS  Google Scholar 

  • Blatter RHE, Jacomet S, Schlumbaum A (2004) About the origin of European spelt (Triticum spelta L.): allelic differentiation of the HMW glutenin B1-1 and A1-2 subunits genes. Theor Appl Genet 108:360–367. doi:10.1007/s00122-003-1441-7

    Article  PubMed  CAS  Google Scholar 

  • Branlard G, Autran JC, Monneveux P (1989) High-molecular-weight glutenin subunit in durum wheat (T. durum). Theor Appl Genet 78:353–358. doi:10.1007/BF00265296

    Article  CAS  Google Scholar 

  • Branlard G, Dardevet M, Amiour N, Igrejas G (2003) Allelic diversity of HMW and LMW glutenin subunits and omegagliadins in French bread wheat (Triticum aestivum L.). Genet Resour Crop Evol 50:669–679. doi:10.1023/A:1025077005401

    Article  CAS  Google Scholar 

  • Caballero L, Martin LM, Alvarez JB (2001) Allelic variation of the HMW glutenin subunits in Spanish accessions of spelt wheat (Triticum aestivum ssp. spelta L. em. Thell). Theor Appl Genet 103:124–128. doi:10.1007/s001220100565

    Article  CAS  Google Scholar 

  • Caballero L, Martin LM, Alvarez JB (2004a) Intra- and interpopulation diversity for HMW glutenin subunits in Spanish spelt wheat. Genet Resour Crop Evol 51:175–181. doi:10.1023/B:GRES.0000020859.62504.e7

    Article  CAS  Google Scholar 

  • Caballero L, Martin LM, Alvarez JB (2004b) Variation and genetic diversity for gliadins in Spanish spelt wheat accessions. Genet Resour Crop Evol 51:679–686. doi:10.1023/B:GRES.0000034575.97497.6e

    Article  CAS  Google Scholar 

  • Ciaffi M, Lifiandra D, Porceddu E, Benedettelli S (1993) Storage protein variation in wild emmer wheat (Triticum turgidum ssp. dicoccoides) from Jordan and Turkey. I. Electrophoretic characterization of genotypes. Theor Appl Genet 86:474–480. doi:10.1007/BF00838563

    Article  CAS  Google Scholar 

  • Ciaffi M, Dominici L, Lafiandra D (1998) High molecular weight glutenin subunit variation in wild and cultivated einkorn wheats. Plant Syst Evol 209:123–137. doi:10.1007/BF00991528

    Article  CAS  Google Scholar 

  • Degaonkar AM, Tamhankar SA, Rao VS (2005) An assessment of cultivated emmer germplasm for gluten proteins. Euphytica 145:49–55. doi:10.1007/s10681-005-0142-5

    Article  CAS  Google Scholar 

  • Dvorak J, Pantaleo DT, Zhang HB, Resta P (1993) The evolution of polyploid wheats: identification of the A genome donor species. Genome 36:21–31. doi:10.1139/g93-004

    Article  PubMed  CAS  Google Scholar 

  • Edwards NM, Gianibelli MC, McCaig TN, Clarke JM, Ames NP, Larroque OR et al (2007) Relationships between dough strength, polymeric protein quantity and composition for diverse durum wheat genotypes. J Cereal Sci 45:140–149. doi:10.1016/j.jcs.2006.07.012

    Article  CAS  Google Scholar 

  • He GY, Rooke L, Steele S, Békés F, Gras P, Tatham AS et al (1999) Transformation of pasta wheat (Triticum turgidum L. var. durum) with high-molecular-weight glutenin subunit genes and modification of dough functionality. Mol Breed 5:377–386. doi:10.1023/A:1009681321708

    Article  CAS  Google Scholar 

  • Igrejas G, Guedes-Pinto H, Carnide V, Branlard G (1999) The high and low molecular weight glutenin subunits and ω-gliadin composition of bread and durum wheats commonly grown in Portugal. Plant Breed 118:297–302. doi:10.1046/j.1439-0523.1999.00378.x

    Article  CAS  Google Scholar 

  • Lafiandra D, Tucci GF, Pavoni A, Turchetta T, Margiotta B (1997) PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat. Theor Appl Genet 94:235–240. doi:10.1007/s001220050405

    Article  CAS  Google Scholar 

  • Lagudah ES, Flood RG, Halloran GM (1987) Variation in high molecular weight glutenin subunits in landraces of hexaploid wheat from Afghanistan. Euphytica 36:3–9. doi:10.1007/BF00730641

    Article  CAS  Google Scholar 

  • Lawrence GJ (1986) The high-molecular-weight glutenin subunit composition of Australian wheat cultivars. Aust J Agric Res 37:125–133. doi:10.1071/AR9860125

    Article  CAS  Google Scholar 

  • Lei ZS, Gale KR, He ZH, Gianibelli C, Larroque O, Xia XC et al (2007) Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat. J Cereal Sci 43:94–101. doi:10.1016/j.jcs.2005.08.003

    Article  Google Scholar 

  • Lefebvre J, Mahmoudi N (2007) The pattern of the linear viscoelastic behaviour of wheat flour dough as delineated from the effects of water content and high molecular weight glutenin subunits composition. J Cereal Sci 45:49–58. doi:10.1016/j.jcs.2006.06.005

    Article  CAS  Google Scholar 

  • Levy AA, Feldman M (1988) Ecogeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat Triticum turgidum var. dicoccoides. Theor Appl Genet 75:651–658. doi:10.1007/BF00289134

    Article  CAS  Google Scholar 

  • Levy AA, Feldman M (1989a) Location of genes for high grain protein percentage and other quantitative traits in wild wheat Triticum turgidum var. dicoccoides. Euphytica 41:113–122. doi:10.1007/BF00022420

    Article  Google Scholar 

  • Levy AA, Feldman M (1989b) Intra- and inter-population variation in grain protein percentage in wild tetraploid wheat, Triticum turgidum var. dicoccoides. Euphytica 42:251–258

    Google Scholar 

  • Li QY, Yan YM, Wang AL, An XL, Zhang YZ, Hsam SLK et al (2006) Detection of HMW glutenin subunit variations among 205 cultivated emmer accessions (Triticum turgidum ssp. dicoccum). Plant Breed 125:120–124. doi:10.1111/j.1439-0523.2006.01173.x

    Article  CAS  Google Scholar 

  • Liu Y, Xiong ZY, He YG, Shewry PR, He GY (2007) Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genet Resour Crop Evol 54:865–874. doi:10.1007/s10722-006-9154-9

    Article  CAS  Google Scholar 

  • Lookhart GL, Hagman K, Kasarda DD (1993) High-molecular-weight glutenin subunits of the most commonly grown wheat cultivars in the U.S. in 1984. Plant Breed 110:48–62. doi:10.1111/j.1439-0523.1993.tb00568.x

    Article  CAS  Google Scholar 

  • Moczulski M, Salmanowicz BP (2003) Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers. J Appl Genet 44:459–471

    PubMed  Google Scholar 

  • Moonen JHE, Scheepstra A, Graveland A (1983) The positive effects of the high molecular weight subunits 5+10 and 2* of glutenin on the bread-making quality of wheat cultivars. Euphytica 32:735–742. doi:10.1007/BF00042153

    Article  Google Scholar 

  • Moragues M, Zarco-Hernández J, Moralejo MA, Royo C (2006) Genetic diversity of glutenin protein subunits composition in durum wheat landraces [Triticum turgidum ssp. turgidum convar. durum (Desf.) Mackey] from the Mediterranean basin. Genet Resour Crop Evol 53:993–1002. doi:10.1007/s10722-004-7367-3

    Article  CAS  Google Scholar 

  • Morgunov AI, Rogers WJ, Sayers EJ, Metakovsky EV (1990) The high-molecular-weight glutenin subunit composition of Soviet wheat varieties. Euphytica 51:41–52. doi:10.1007/BF00022891

    Article  CAS  Google Scholar 

  • Nakamura H (2000) Allelic variation at high-molecular-weight glutenin subunit loci, Glu-A1, Glu-B1 and Glu-D1, in Japanese and Chinese hexaploid wheats. Euphytica 112:187–193. doi:10.1023/A:1003888116674

    Article  CAS  Google Scholar 

  • Nakamura H (2001) Genetic diversity of high-molecular-weight glutenin subunit compositions in landraces of hexaploid wheat from Japan. Euphytica 120:227–234. doi:10.1023/A:1017514423061

    Article  CAS  Google Scholar 

  • Nakamura H, Fujimaki H (2002) Specific Glu-D1f allele frequency of Japanese common wheat compared with distribution of Glu-1 alleles in Chinese wheat. Cereal Chem 79:486–490. doi:10.1094/CCHEM.2002.79.4.486

    Article  CAS  Google Scholar 

  • Nakamura H, Inazu A, Hirano H (1999) Allelic variation in high-molecular-weight glutenin subunit loci of Glu-1 in Japanese common wheats. Euphytica 106:131–138. doi:10.1023/A:1003516620466

    Article  CAS  Google Scholar 

  • Ng PKW, Bushuk W (1987) Glutenin of Marquis wheat as a reference for estimating molecular weights of glutenin subunits by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cereal Chem 64:324–327

    CAS  Google Scholar 

  • Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex loci, Glu-A1, Glu-B1 and Glu-D1 which coded for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35

    Google Scholar 

  • Payne PI, Corfield KG, Blackman JA (1979) Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bead-making quality in wheats of related pedigree. Theor Appl Genet 55:153–159. doi:10.1007/BF00295442

    Article  CAS  Google Scholar 

  • Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120. doi:10.1007/BF00263101

    Article  CAS  Google Scholar 

  • Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high molecular weight subunits of wheat glutenin. 1. Allelic variation in subunits among cultivars of wheat. Theor Appl Genet 60:229–236. doi:10.1007/BF02342544

    Article  CAS  Google Scholar 

  • Payne PI, Nightingale MA, Krattiger AF (1987) The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J Sci Food Agric 40:51–65. doi:10.1002/jsfa.2740400108

    Article  CAS  Google Scholar 

  • Payne PI, Corfield KG, Holt LM, Blackman JA (1991) Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J Sci Food Agric 32:51–60. doi:10.1002/jsfa.2740320109

    Article  Google Scholar 

  • Pflüger LA, Martin LM, Alvarez JB (2001) Variation in the HMW and LMW glutenin subunits from Spanish accessions of emmer wheat (Triticum turgidum ssp. dicoccum Schrank). Theor Appl Genet 102:767–772. doi:10.1007/s001220051708

    Article  Google Scholar 

  • Piergiovanni AR, Blanco A (1999) Variation of HMW glutenin and γ-gliadin subunits in selected accessions of Triticum dicoccum (Schrank) and T. spelta (L.). Cereal Res Commun 27:205–211

    CAS  Google Scholar 

  • Rayfuse LM, Jones SS (1993) Variation at Glu-1 loci in club wheat. Plant Breed 111:89–98. doi:10.1111/j.1439-0523.1993.tb00613.x

    Article  CAS  Google Scholar 

  • Rodriguez-Quijano M (1990) Variation of high-molecular-weight glutenin subunits in Spanish landraces of Triticum aestivum ssp. vulgare and ssp. spelta. J Genet Breed 44:121–126

    Google Scholar 

  • Shan XY, Clayshulte SR, Haley SD, Byrne PF (2007) Variation for glutenin and waxy alleles in the US hard winter wheat germplasm. J Cereal Sci 45:199–208. doi:10.1016/j.jcs.2006.09.007

    Article  CAS  Google Scholar 

  • Shewry PR, Halford NG, Tatham AS, Popineau Y, Lafiandra D, Belton PS (2003a) The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv Food Nutr Res 45:219–302. doi:10.1016/S1043-4526(03)45006-7

    Article  PubMed  CAS  Google Scholar 

  • Shewry PR, Gilbert SM, Savage AWJ, Tatham AS, Wan YF, Belton PS et al (2003b) Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum) which is associated with poor end use properties. Theor Appl Genet 106:744–750

    PubMed  CAS  Google Scholar 

  • Sultana T, Ghafoor A, Ashraf M (2007) Genetic variability in bread wheat (Triticum aestivum L.) of Pakistan based on polymorphism for high molecular weight glutenin subunits. Genet Resour Crop Evol 54:1159–1165. doi:10.1007/s10722-007-9242-5

    Article  Google Scholar 

  • Takata K, Yamauchi H, Nishio Z, Kuwabara T (2000) Effect of high molecular weight glutenin subunits on bread-making quality using near-isogenic lines. Breed Sci 50:303–308

    CAS  Google Scholar 

  • Tohver M (2007) High molecular weight (HMW) glutenin subunit composition of some Nordic and Middle European wheats. Genet Resour Crop Evol 54:67–81. doi:10.1007/s10722-005-1885-5

    Article  CAS  Google Scholar 

  • Vallega V (1988) Comparative analysis of high-molecular-weight glutenin subunit composition in various Triticum species. Plant Breed 100:241–246. doi:10.1111/j.1439-0523.1988.tb00249.x

    Article  CAS  Google Scholar 

  • Vallega V, Mello-Sampayo T (1987) Variation of high-molecular-weight glutenin subunits amongst cultivars of Triticum turgidum L. from Portugal. Euphytica 3:755–762. doi:10.1007/BF00051858

    Article  Google Scholar 

  • Vallega V, Waines JG (1987) High-molecular-weight glutenin subunit variation in Triticum turgidum var. dicoccum. Theor Appl Genet 74:706–710. doi:10.1007/BF00247545

    Article  CAS  Google Scholar 

  • Wan Y, Wang D, Shewry PR, Halford NG (2002) Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrical. Theor Appl Genet 104:828–839. doi:10.1007/s00122-001-0793-0

    Article  PubMed  CAS  Google Scholar 

  • Waines JG, Payne PI (1987) Electrophoretic analysis of the high-molecular-weight glutenin subunits of Triticum monococcum, T. urartu, and the A genome of bread wheat (T. aestivum). Theor Appl Genet 74:71–76. doi:10.1007/BF00290086

    Article  CAS  Google Scholar 

  • Wei YM, Zheng YL, Liu DC, Zhou YH, Lan XJ (2000) Gliadin and HMW-glutenin variations in Triticum turgidum L. ssp. turgidum and T. aestivum L. landraces native to Sichuan, China. Wheat Inf Serv 90:13–20

    Google Scholar 

  • Wei YM, Zheng YL, Liu DC, Zhou YH, Lan XJ (2002) HMW-glutenin and gliadin variations in Tibetan weedrace, Xinjiang rice wheat and Yunnan hulled wheat. Genet Resour Crop Evol 49:327–330. doi:10.1023/A:1015544403431

    Article  Google Scholar 

  • Yan Y, Hsam SLK, Yu JZ, Jiang Y, Ohtsuka I, Zeller FJ (2003) HMW and LMW glutenin alleles among putative tetraploid and hexaploid European spelta wheat (Triticum spelta L.) progenitors. Theor Appl Genet 107:1321–1330. doi:10.1007/s00122-003-1315-z

    Article  PubMed  CAS  Google Scholar 

  • Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot 129:157

    Google Scholar 

  • Zhang LY, Ravel C, Bernard M, Balfourier F, Leroy P, Feuillet C et al (2006) Transferable bread wheat EST-SSRs can be useful for phylogenetic studies among the Triticeae species. Theor Appl Genet 113:407–418. doi:10.1007/s00122-006-0304-4

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National High Technology Research and Development Program of China (863 program 2006AA10Z179 and 2006AA10Z1F8), the Key Technologies R&D Program of China (2006BAD01A02-23 and 2006BAD13B02).

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  1. Key Laboratory of Crop Genetic Resources and Improvement in Southwest China, Ministry of Education, Sichuan Agricultural University, Yaan, Sichuan, 625014, China

    Li-Li Xu, Wei Li, Yu-Ming Wei & You-Liang Zheng

  2. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, 611830, China

    Li-Li Xu, Wei Li, Yu-Ming Wei & You-Liang Zheng

  3. College of Agronomy, Sichuan Agricultural University, Yaan, Sichuan, 625014, China

    Wei Li

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  1. Li-Li Xu
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  2. Wei Li
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Correspondence to Wei Li or You-Liang Zheng.

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Xu, LL., Li, W., Wei, YM. et al. Genetic diversity of HMW glutenin subunits in diploid, tetraploid and hexaploid Triticum species. Genet Resour Crop Evol 56, 377–391 (2009). https://doi.org/10.1007/s10722-008-9373-3

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