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Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan

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Abstract

Accurate and reliable means for identification are necessary to assess the discrimination between landraces of tetraploid wheat [T.␣turgidum L. subsp. durum (Desf.) Husn.] and hexaploid wheat (T. aestivum L. em. Thell.). In Afghanistan, farmers usually cultivate mixed landraces, and thus distinction between bread and durum is difficult. A set of 18 microsatellites derived from the DuPont EST-database were used to describe genetic diversity in a sample of 82 Afghan wheat landraces. A total of 101 alleles were detected, with allele number per locus ranging from 2 to 13, and a mean allele number of 6.31. The percentage of polymorphic loci was 89%. The EST-SSRs markers showed different level of gene diversity: the highest Polymorphism Information Content value (0.921) was observed with DuPw 221. Our results demonstrated that with a reasonable number of expressed sequences target microsatellites (EST-SSRs) it is possible to discriminate between T. durum and T. aestivum species of wheat germplasm. Our results showed that EST-databases could be a useful source for species-specific markers and have the potential for new genic microsatellites markers that could enhance screening germplasm in gene banks.

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Acknowledgements

The work has been supported by International Development of Research Center (IDRC) and the International Center for Agricultural Research in the Dry Areas (ICARDA). We thank Drs. M. Nachit and M. Baum for their comments and reviewing the paper. The authors want to thank Dr. J. Ryan for editing the manuscript.

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Authors and Affiliations

  1. Genetic Resources Unit, International Center for Agricultural Research in the Dry Areas, PO Box 5466, Aleppo, Syria

    K. Chabane & J. Valkoun

  2. Integrated Gene Management Program, MP2, ICARDA, PO Box 5466, Aleppo, Syria

    O. Abdalla & H. Sayed

Authors
  1. K. Chabane
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  2. O. Abdalla
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  3. H. Sayed
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  4. J. Valkoun
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Correspondence to K. Chabane.

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Chabane, K., Abdalla, O., Sayed, H. et al. Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan. Genet Resour Crop Evol 54, 1073–1080 (2007). https://doi.org/10.1007/s10722-006-9193-2

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