Abstract
The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic and transferable SSRs were also identified.
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Abbreviations
- SSRs:
-
Simple sequence repeats
- RFLP:
-
Restriction Fragment Length Polymorphism
- CTAB:
-
Cetyl Trimethyl Ammonium Bromide
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Acknowledgements
We are grateful to Prof. Guo-ying Wang for providing information of ESTs in foxtail millet. The work was supported by the grants No. 2005CCA02500 and 2005DIA4J019.
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Jia, XP., Shi, YS., Song, YC. et al. Development of EST-SSR in foxtail millet (Setaria italica). Genet Resour Crop Evol 54, 233–236 (2007). https://doi.org/10.1007/s10722-006-9139-8
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DOI: https://doi.org/10.1007/s10722-006-9139-8