Abstract
The present investigation was carried out on fifteen germplasm lines of Pisum sativum L. were used for characterization using Randomly Amplified Polymorphic DNA (RAPD) markers. While 12 random primers were taken, out of them 11 primers gave amplification. These primers gave a total of 133 bands out of which 106 were polymorphic. Genetic similarities of the RAPD profiles were estimated by using Jaccard’s coefficient with NTSYSpc 2.0 software. The similarity index values ranged from 0.263 to 0.793 indicating the presence of enormous genetic diversity at molecular level. A dendrogram generated by cluster analysis divided fifteen fieldpea genotypes into two Groups A and B. Major Group A have five genotypes and major Group B have nine genotypes.
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References
Ayliffe AM, Lawrence GJ, Ellis JG, Pryor AJ (1994) Heteroduplex molecules formed between allelic sequences cause non parental RAPD bands. Nucleic Acid Res 22:1632–1636
Hunt GJ, Page RE (1992) Patterns of inheritance with RAPD molecular markers reveal novel type of polymorphism in honey bee. Theor Appl Genet 85:15–20
Lee M (1995) DNA marker and plant breeding programs. Adv Agron 57:265–326
Miller JC, Tanksley SP (1990) RFLP analysis of polygenetic relationship and genetic variation in the genus Lycopersicon. Theor Appl Genet 80:437–448
Munthali M, Ford-Lloyd BV, Nembury HJ (1992) The random amplification of polymorphic DNA for fingerprinting plants. PCR Method Appl 1:274–276
Murray MG, Thompson WF (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res. 8:4321–4325
Pandian A, Rebecca F, Paul Taylor WJ (2000) Transferability of Sequence Tagged Microsatellite Site (STMS) Primers across Four Major Pulses. Plant Molecular Biology Reporter 18:395a–395h
Riedy MF, Hamilton WJ, Aquadro FC (1992) Excess of non parental bands in offspring’s from known primate pedigrees assayed using RAPD PCR. Nucleic Acid Res 20:918
Rohlf FJ (1992) NTSYS-pc Numerical taxonomy and multivariate analysis system. Exeter Software, New York, USA
Sambrook T, Fritsch EF, Maniathis T (1989) Molecular cloning :A laboratory manual. 2nd edn, vol. 3. Cold Spring Harbour Laboratory, Cold Spring Harbour, NY
Virk PS, Fordlloyd BV, Jackson MT, John H (1995) Use of RAPD for the study of diversity within plant germplasm collections. Heredity 74:170–179
Whitkins, SE, Isaac PG, Slater RJ (1994). Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theor Appl Genet 86:497–504
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tringey SV (1990) DNA polymorphism applied by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18:6531–6535
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Yadav, V.K., Kumar, S. & Panwar, R.K. Measurement of genetic dissimilarity in fieldpea (Pisum sativum L.) genotypes using RAPD markers. Genet Resour Crop Evol 54, 1285–1289 (2007). https://doi.org/10.1007/s10722-006-9109-1
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DOI: https://doi.org/10.1007/s10722-006-9109-1