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Genetic diversity of Guizotia abyssinica (L. f.) Cass. (Asteraceae) from Ethiopia as revealed by random amplified polymorphic DNA (RAPD)

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Abstract

Genetic diversity of 70 populations of niger (Guizotia abyssinica) representing all its growing regions in Ethiopia was investigated using random amplified polymorphic DNA (RAPD) to reveal the extent of its populations genetic diversity. Ninety-seven percent of the loci studied was revealed to be polymorphic for the whole data set. The within population diversity estimated by Shannon diversity index and Nei gene diversity estimates was revealed to be 0.395 and 0.158, respectively. The extent of genetic variation of populations from major niger producing regions was significantly lower than that of populations from other regions; however, it is distributed regardless of altitude of growth. Genetic differentiation between populations was estimated with Shannon index as G′ ST (0.432), Nei’s G ST (0.242) and AMOVA based F ST (0.350) and appears to be equivalent to the average values calculated from various RAPD based studies on outcrossing species. Higher proportion of the variation detected by AMOVA resided within populations (64.58%) relative to the amount of variation among populations (35.42%). UPGMA cluster analysis showed that most of the populations were clustered according to their region of origin. However, some populations were genetically distant from the majority and seem to have unique genetic properties. It is concluded that the crop has a wide genetic basis that may be used for the improvement of the species through conventional breeding and/or marker assisted selection. Collection of germplasm from areas not yet covered and/or underrepresented is the opportunity to broaden the genetic basis of genebank collection.

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Authors and Affiliations

  1. Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, Alnarp, SE 230 53, Sweden

    Mulatu Geleta & Tomas Bryngelsson

  2. Department of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

    Mulatu Geleta, Endashaw Bekele & Kifle Dagne

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  1. Mulatu Geleta
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  2. Tomas Bryngelsson
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  3. Endashaw Bekele
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  4. Kifle Dagne
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Correspondence to Mulatu Geleta.

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Geleta, M., Bryngelsson, T., Bekele, E. et al. Genetic diversity of Guizotia abyssinica (L. f.) Cass. (Asteraceae) from Ethiopia as revealed by random amplified polymorphic DNA (RAPD). Genet Resour Crop Evol 54, 601–614 (2007). https://doi.org/10.1007/s10722-006-0018-0

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