Abstract
Atriplex halimus L. (Chenopodiaceae) (Saltbush) is a perennial species used as a fodder shrub for livestock in arid and semi-arid areas, particularly in North Africa. The aim of this work was to determine whether differences in ploidy level and/or nuclear DNA content exist among populations from widely-separated sites in Tunisia. We determined nuclear DNA content and chromosome numbers for populations of A. halimus from seven different locations (Gabes, Medenine, Tataouine, Monastir, Tunis, Sidi Bouzid, Kairouan). The chromosome counts showed that all the Tunisian populations, plus a population from Eraclea (Italy), were tetraploid (2n = 4x = 36) whereas a population from Cala Tarida (Spain) was diploid (2n = 2x = 18). With respect to nuclear DNA, the 2C DNA content of population Cala Tarida was estimated to be 2.41 pg. There was no significant difference among the tetraploid populations (or among plants within populations), whose 2C DNA content ranged from 4.92 to 4.97 pg.
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References
A. Abbad M. Cherkaoui A. Benchaabane (2003) ArticleTitleMorphology and allozyme variability of three natural populations of Atriplex halimus L Ecologica Mediterranea 29 99–109
M.D. Bennett P. Bhandol I.J. Leitch (2000) ArticleTitleNuclear DNA amounts in angiosperms and their modern uses – 807 new estimates Ann. Bot. 86 859–909 Occurrence Handle10.1006/anbo.2000.1253 Occurrence Handle1:CAS:528:DC%2BD3cXmsFKmu7w%3D
J. Doležel (1997) ArticleTitleApplication of flow cytometry for study of plant genomes J. Appl. Genet. 38 285–302
J. Doležel S. Sgorbati S. Lucretti (1992) ArticleTitleComparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants Physiol. Plant. 85 625–631
A. Franclet H.N. Houérou ParticleLe (1971) Les Atriplex en Tunisie et en Afrique du Nord Food and Agriculture Organization Rome
A. Haddioui M. Baaziz (2001) ArticleTitleGenetic diversity of natural populations of Atriplex halimus L. in Morocco: An isoenzyme-based overview Euphytica 121 99–106 Occurrence Handle10.1023/A:1012051222530
W.J. Katembe I.A. Ungar J.P. Mitchell (1998) ArticleTitleEffect of salinity on germination and seedling growth of two Atriplex species (Chenopodiaceae) Ann. Bot. 82 167–175 Occurrence Handle10.1006/anbo.1998.0663
H.N. Houérou ParticleLe (1981) Impact of man and his animals on Mediterranean vegetation F. Castri Particledi D.W. Goodall R.L. Specht (Eds) Ecosystems of the World. 11. Mediterranean-type Shrublands Elsevier Amsterdam 479–521
H.N. Houérou ParticleLe (1992) ArticleTitleThe role of saltbushes (Atriplex spp.) in arid land rehabilitation in the Mediterranean Basin: A review Agrofor. Syst. 18 107–148 Occurrence Handle10.1007/BF00115408
M.A. Nobs (1975) ArticleTitleChromosome numbers in Atriplex Carnegie Institute of Washington yearbook 74 762
O. Nuñez (1968) ArticleTitleAn acetic-hematoxylin squash method for small chromosomes Caryologia 21 115–119
C.B. Osmond O. Björkman D.J. Anderson (1980) Physiological Processes in Plant Ecology Springer-Verlag Berlin
H.C. Stutz S.C. Sanderson (1983) ArticleTitleEvolutionary studies of Atriplex: chromosome races of A. confertifolia (Shadscale) Amer. J. Bot. 77 490–498
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Hcini, K., Walker, D.J., Bouzid, S. et al. Determination of Ploidy Level and Nuclear DNA Content in Tunisian Populations of Atriplex halimus L.. Genet Resour Crop Evol 53, 1–5 (2006). https://doi.org/10.1007/s10722-005-5806-4
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DOI: https://doi.org/10.1007/s10722-005-5806-4