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Chloroplast Microsatellites to Investigate the Origin of Grapevine

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Abstract

The origin of the grapevine, Vitis vinifera ssp . sativa L., has been investigated with archaeobotanical–archaeological, cultural and historical data indicating a unique domestication centre located in the Caucasian and Middel-East regions about 6–7000 years ago, but, events leading to the domestication of this species are still an open issue. In this work, eight universal chloroplast microsatellites are used to assess genetic relationships among varieties selected as representatives of four distinct geographical groups from Middle-East to Western European regions. Results show that two out of the eight analysed chloroplast loci are polymorphic within the 142 individuals. Allele variants of the cpSSR loci combine in a total of six different haplotypes. The analysis of haplotypes distribution and haplotype diversity (HD) suggest that only three out of the six haplotypes are represented in the Caucasian and Middle-East samples, with 90% of individuals sharing the same haplotype. Moreover, the presence of all six haplotypes in the European accessions, with a high level of haplotype diversity, suggests varietal influx in these areas. Concerning the Western European varieties, especially in Spanish accessions, half of the individuals share haplotype VI which is completely absent in the Caucasian and Middle-East cultivars. This result opens the discussion about the existence of a unique and common domestication centre, located in the Caucasian and Middle-East area, for all the European cultivars.

This work suggests the usefulness of chloroplast genome markers to provide information on haplotype distributions that could help to identify further geographical areas for grapevine varietal evolution.

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Abbreviations

cpSSRs:

chloroplast simple sequence repeats

References

  • M.K. Aradhya G.S. Dangl B.H. Prins J.M. Boursiquot M.A. Walker C.P. Meredith C.J. Simon (2003) ArticleTitleGenetic structure and differentiation in cultivated grapeVitis vinifera L Genet. Res. Camb. 81 179–182 Occurrence Handle1:CAS:528:DC%2BD3sXltFKlt7s%3D

    CAS  Google Scholar 

  • R. Botta N.S. Scott I. Eynard M.R. Thomas (1995) ArticleTitleEvaluation of microsatellite sequence-tagged site markers for characterising Vitis vinifera cultivars Vitis 34 99–102

    Google Scholar 

  • J.E. Bowers G.S. Dangl R. Vignani C.P. Meredith (1996) ArticleTitleIsolation and characterisation of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.) Genome 39 628–633 Occurrence Handle1:CAS:528:DyaK28XlvVWmt7Y%3D

    CAS  Google Scholar 

  • S. Dumolin B. Demesure R.J. Petit (1995) ArticleTitleInheritance of chloroplast and mitochondrial genomes in pedunculate oak investigated with an efficient PCR method Theor. Appl. Genet. 91 1253–1256 Occurrence Handle1:CAS:528:DyaK28XhsFKmsLo%3D Occurrence Handle10.1007/BF00220937

    Article  CAS  Google Scholar 

  • G. Forni (1990) Gli albori dell’agricoltura REDA RomaItaly

    Google Scholar 

  • T. Fossati M. Labra S. Castglione O. Failla A. Scienza F. Sala (2001) ArticleTitleThe use of AFLP and SSR molecular markers to decipher homonyms and synomyms in grapevine cultivars: the case of the varietal group known as ȁ8Schiave’ Theor. Appl. Genet. 102 200–205 Occurrence Handle1:CAS:528:DC%2BD3MXisVyns74%3D Occurrence Handle10.1007/s001220051636

    Article  CAS  Google Scholar 

  • Freimer N.B. and Slatkin M. 1996. Microsatellites: evolution and mutational processes. In: Variation in the Human Genome. Ciba Foundation SymposiumVol. 197. Ciba Foundation, London, pp. 51–72.

  • F. Grassi M. Labra S. Imazio A. Spada S. Sgorbati A. Scienza F. Sala (2003) ArticleTitleEvidence of a secondary grapevine domestication centre detected by SSR analysis Theor. Appl. Genet. 107 1315–1320 Occurrence Handle13679993 Occurrence Handle1:CAS:528:DC%2BD3sXos1OktbY%3D Occurrence Handle10.1007/s00122-003-1321-1

    Article  PubMed  CAS  Google Scholar 

  • F. Grassi M. Labra A. Scienza S. Imazio (2002) ArticleTitleChloroplast SSR markers to assess DNA diversità in wild and cultivated grapevines Vitis 41 157–158 Occurrence Handle1:CAS:528:DC%2BD38XotlOhs7Y%3D

    CAS  Google Scholar 

  • M. Hopf (1991) South and Southwest Europe W. Zeist ParticleVan K. Wasylikowa K.E. Behre (Eds) Progress in Old World Paleoethnobotany Rotterdam-Brookfield Balkema 241–277

    Google Scholar 

  • H. Kroll (1991) Sudosteuropa W. Zeist ParticleVan K. Wasylikowa K.E. Behre (Eds) Progress in Old World Paleoethnobotany Rotterdam-Brookfield Balkema 161–177

    Google Scholar 

  • M. Labra E. Carreno-Sanchez M. Bardini B. Basso F. Sala A. Scienza (2001) ArticleTitleExtraction and purification of DNA from grapevine leaves Vitis 40 101–102 Occurrence Handle1:CAS:528:DC%2BD3MXmtFOjt74%3D

    CAS  Google Scholar 

  • M. Labra O. Failla G. Forni A. Ghiani A. Scienza F. Sala (2002) ArticleTitleMicrosatellites analysis to define genetic diversity of grapevines (Vitis vinifera L.) grown in central and western Mediterranean countries Int. J. Vine Wine 36 11–20 Occurrence Handle1:CAS:528:DC%2BD38XjtlWitrk%3D

    CAS  Google Scholar 

  • M. Labra S. Imazio F. Grassi M. Rossoni S. Citterio S. Sgorbati A. Scienza O. Failla (2003) ArticleTitleMolecular approach to assess the origin of cv. Marzemino Vitis 42 137–140 Occurrence Handle1:CAS:528:DC%2BD3sXos1Wnuro%3D

    CAS  Google Scholar 

  • L. Levadoux (1956) ArticleTitleLes populations sauvages et cultivées de Vitis vinifera Ann. l’ammelior. plantes cultivées 6 50–110

    Google Scholar 

  • Logothetis B.C. 1970. The development of the vines and of viticulture in GreeceTessalonicaAristotelion Panepistemion Tessalonikis (in Greek, with summary in French).

  • J.P. Martin J. Borrego F. Cabello J.M. Ortiz (2003) ArticleTitleCharacterisation of Spanish grapevine cultivar diversity using sequence-tagged microsatellite site markers Genome 46 10–18 Occurrence Handle12669792 Occurrence Handle1:STN:280:DC%2BD3s7ltlaitw%3D%3D Occurrence Handle10.1139/g02-098

    Article  PubMed  CAS  Google Scholar 

  • P.E. McGovern (2003) Ancient Wine: The Search of the Origin of Viniculture Princeton University Press

    Google Scholar 

  • C.P. Meredith (2001) ArticleTitleGrapevine genetics: probing the past and facing the future Agric. Cons. Scient. 66 21–25

    Google Scholar 

  • H.P. Olmo (1976) Grapes N.W. Simmounds (Eds) Evolution of Crop Plants Longman London 294–298

    Google Scholar 

  • A.E. Palmé G. Vendramin (2002) ArticleTitleChloroplast DNA variation, postglacial recolonisation and hybidisation in hazel, Corylus avellana Mol. Ecol. 11 1769–1779 Occurrence Handle12207726 Occurrence Handle10.1046/j.1365-294X.2002.01581.x

    Article  PubMed  Google Scholar 

  • J. Provan W. Powell P.H. Hollingsworth (2001) ArticleTitleChloroplast microsatellites: new tools for studies in plant ecology and evolution Trends Ecol. Evol. 16 142–147 Occurrence Handle11179578 Occurrence Handle10.1016/S0169-5347(00)02097-8

    Article  PubMed  Google Scholar 

  • D. Rivera-Nunez M.J. Walker (1989) ArticleTitleA review of Paleobotanical findings of early Vitis in the Mediterranean and of the origins of cultivated grape-vines, with a special reference to new pointers to prehistoric exploitation in the western Mediterranean Rev. Paleobot. Palin. 51 205–237 Occurrence Handle10.1016/0034-6667(89)90033-X

    Article  Google Scholar 

  • K.M. Sefc F. Lefort M.S. Grando K.D. Scott H. Steinkellner M.R. Thomas (2001) Microsatellite markers for grapevine: a state of the art K.A. Roubelakis-Angelakis (Eds) Molecular Biology and Biotechnology of Grapevine Kluwer Academic Publishers The Netherlands

    Google Scholar 

  • K.M. Sefc M.S. Lopes F. Lefort R. Botta K.A. Roubelakis-Angelakis J. Ibanes I. Pejic H.W. Wagner J. Glossl H. Steinkellner (2000) ArticleTitleMicrosatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars Theor. Appl. Genet. 100 498–505 Occurrence Handle10.1007/s001220050065

    Article  Google Scholar 

  • K.M. Sefc F. Regner J. Gossl H. Steinkellner (1998) ArticleTitleGenotyping of grapevine and rootstock cultivars using microsatellite markers Vitis 37 15–20

    Google Scholar 

  • K.M. Sefc H. Steinkellner F. Lefort R. Botta A. Camara Machado J. Borrego E. Maleti J. Glssl (2003) ArticleTitleEvaluation of the genetic contribution of local wild vines to European germplasm Am. J. Enol. Vitic. 54 15–21

    Google Scholar 

  • A.M. Valdes M. Slatkin N.B. Freiner (1993) ArticleTitleAllele frequencies at microsatellite loci: the stepwise mutation model revisited Genetics 133 737–749 Occurrence Handle8454213 Occurrence Handle1:STN:280:ByyB3c3kvVA%3D

    PubMed  CAS  Google Scholar 

  • G.G. Vendramin B. Ziegenhagen (1997) ArticleTitleCharacterisation and inheritance of polymorphic plastid microsatellites in Abies Genome 40 857–864 Occurrence Handle9449797 Occurrence Handle1:CAS:528:DyaK1cXotVKnuw%3D%3D

    PubMed  CAS  Google Scholar 

  • K. Wasilykowa (1991) East Central Europe W. Van Zeist K. Wasilykowa K.E. Behre (Eds) Progress in Old World Palaeoethnobotany Rotterdam-Brookfield Balkema 161–177

    Google Scholar 

  • K. Weising R. Gardner (1999) ArticleTitleA set of conserved PCR primers for the analysis of simple sequence repeat polymorphism in chloroplast genomes of dicotyledonous angiosperms Genome 42 9–19 Occurrence Handle10207998 Occurrence Handle1:CAS:528:DyaK1MXhsVyqsb0%3D Occurrence Handle10.1139/gen-42-1-9

    Article  PubMed  CAS  Google Scholar 

  • D. Zohary M. Hopf (1993) Domestication of plants in the old world Clarendon Press Oxford 143–150

    Google Scholar 

  • D. Zohary P. Spiegel-Roy (1975) ArticleTitleBeginnings of fruit growing in the old world Science 187 319–327

    Google Scholar 

Download references

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Imazio, S., Labra, M., Grassi, F. et al. Chloroplast Microsatellites to Investigate the Origin of Grapevine. Genet Resour Crop Evol 53, 1003–1011 (2006). https://doi.org/10.1007/s10722-004-6896-0

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  • DOI: https://doi.org/10.1007/s10722-004-6896-0

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