Abstract
A total of 359 accessions of oil palm (Elaeis guineensis Jacq.) originating from 11 African countries (Nigeria, Cameroon, Congo DR, Tanzania, Angola, Senegal, Sierra Leone, Guinea, Ghana, Madagascar and Gambia) were characterized using the RFLP method using the standard Deli dura as the check. Genomic DNA from each sample was digested using five restriction enzymes and hybridized with four oil palm cDNA probes. Data were analyzed using Biosys-1 computer software to calculate the genetic variability parameters. In general, all the collections exhibited higher levels of diversity than the standard variety, Deli dura. The standard variety, Deli dura, lost 36 alleles as compared to the natural populations indicating a reduction in genetic variability. Material from Nigeria showed the highest mean number of alleles per locus (1.9) and percentage of polymorphic loci (67.2%). These findings, combined with others, suggest that Nigeria may be the center of diversity of wild oil palm. It further suggests that oil palm natural populations maybe possessing adequate genetic variability that are potentially useful for improvement programs.
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References
A.H.D. Brown (1978) ArticleTitleIsoenzymeplant population structure and genetic conservation Theor. Appl. Genet. 52 145–157 Occurrence Handle10.1007/BF00282571
K. Chaisurisri Y.A. El-Kassaby (1994) ArticleTitleGenetic diversity in a seed production population vs. natural population of Sitka spruce Biodiv. Conserv. 3 512–523
Cheah S.C. 1994. A paper presented in PORIM Annual Research Seminar27 August 1994. Molecular probe technology for the oil palm. Biology Division, PORIM, BangiSelangor.
Cheah S.C., Siti Nor Akmar A., Ooi L.C.L., Rahimah A.R. and Maria M. 1990. A paper presented at the Third National Molecular Biology SeminarSeptember 25–26, 1990. Restriction fragment length polymorphism in oil palm. Serdang, SelangorMalaysia.
Cheah S.C., Ooi L.C.L., Rahimah A.R. and Maria M. 1993. A paper presented at the Workshop on Technique and Instrumentation for Plant Physiology, September 21–22, 1993. Application of molecular marker technique in the oil palm. Universiti MalayaKuala LumpurMalaysia.
S.C. Dellaporta J. Woods J.B. Hicks (1983) ArticleTitleA plant DNA minipreparation: version II Plant Mol. Biol. 1 19–21 Occurrence Handle1:CAS:528:DyaL2cXksFWhtrk%3D
L.E. Eguiarte N. Perez-Nasser D. Pinero (1992) ArticleTitleGenetic structureoutcrossing rate and heterosis in Astrocaryum mexicanum (tropical palm): implication for evolution and conservation Heredity 69 217–228 Occurrence Handle1:CAS:528:DyaK3sXhvFeqtA%3D%3D
Y.A. El-Kassaby K. Ritland (1996) ArticleTitleImpact of selection and breeding of the genetic diversity in Douglas-fir Biodiv. Conserv. 5 795–813
N.C. Ellstrand D.R Elam (1993) ArticleTitlePopulation genetic consequences of small population size: Implications for plant conservation Ann. Rev. Ecol. Syst. 24 217–242 Occurrence Handle10.1146/annurev.es.24.110193.001245
R. Frankham (1997) ArticleTitleDo island populations have less genetic variation than mainland populations? Heredity 78 311–327 Occurrence Handle10.1038/sj.hdy.6880980 Occurrence Handle9119706
D.J. Futuyama (1986) Evolutionary Biology Sinauer SunderlandMass
M. Ghesquiere (1984) ArticleTitleEnzyme polymorphism in oil palm I – genetic control of nine enzyme systems Oleagineux. 39 IssueID12 561–574 Occurrence Handle1:CAS:528:DyaL2MXlt1Siur0%3D
M. Ghesquiere (1985) ArticleTitleEnzyme polymorphism in oil palm (Elaeis guineensis Jacq.) Oleagineux 40 IssueID11 529–540 Occurrence Handle1:CAS:528:DyaL28Xkt1Wrtrc%3D
J.L. Hamrick M.J.W. Godt (1989) Allozyme diversity in plant species A.D.H. Brown M.T. Clegg A.L. Kahler B.S. Weir (Eds) Plant Population Genetic, Breeding and Genetic Resources Sinauer Associates Sunderland 43–63
C.W.C. Hartley (1988) The oil palm (E. guineensis Jacq.). 3rd ed Longman Group Limited New York
J.G. Hawkes (1983) The Diversity of Crop Plants Harvard University Press Cambridge
Hayati A. 2002. Isozyme variation study in African oil palm (E. guineensis Jacq.) germplasm collections. MSc. thesis. Universiti Kebangsaan MalaysiaMalaysia.
M. He A. Wilde M.A. Kaderbhai (1990) ArticleTitleA simple-step procedure for small-scale preparation of Escherichia coli plasmids Nucleic Acids Res. 18 IssueID6 1660 Occurrence Handle1:CAS:528:DyaK3cXhvFCltL8%3D Occurrence Handle2183205
P.L. Jack T.A.F. Dimitrijevic S. Mayes (1995) ArticleTitleAssessment of nuclearmitochondrial and chloroplast RFLP markers in oil palm (Elaeis guineensis) Theor. Appl. Genet. 90 643–649 Occurrence Handle10.1007/BF00222128
P.L. Jack S. Mayes (1993) ArticleTitleUse of molecular markers for oil palm breeding II. Use of DNA markers (RFLPs) Oleagineux 48 1–8 Occurrence Handle1:CAS:528:DyaK3sXkvVOgsL8%3D
Kularatne W.W.P.W.M.R.S. 2000. Assessment of genetic diversity in natural oil palm (Elaeis guineensis Jacq.) populations using amplified fragment length polymorphism markers. PhD. thesis, University Kebangsaan MalaysiaKuala Lumpur.
A. Kushairi N. Rajanaidu B.S. Jalani (2002) ArticleTitleGenetic variation of iodine valuecarotene and tocotrienols in palm oil and its application in pharmaceuticals Malaysian Oil Sci. Technol. 1 IssueID2 92–105
Kushairi A., Rajanaidu N., Mohd Din A., Isa Z.A., Noh A. and Junaidah J. 2003a. Palm Series 5 (PS5) – Breeding populations selected for thin shell teneras. MPOB Information Series 183. MPOBBangiSelangor.
Kushairi A., Rajanaidu N., Mohd Din A., Isa Z.A., Noh A. and Junaidah J. 2003b. Palm Series 6 (PS6) – Breeding populations selected for large fruit duras. MPOB Information Series 184. MPOBBangiSelangor.
D.A. Levin (1976) ArticleTitleConsequences of long-term artificial selection, inbreeding and isolation in Phlox II. The organization of allozymic variability Evolution 30 463–472
C.R. Lopez S.F.s Doscriss M.A. Ferreira M.C. Moretzsohnn (1992) ArticleTitleGenetics of the genus Acrocomia (palmae) III Misrogeographical genetic variability in isozyme frequencies J. Genet. Breed. 46 IssueID1 9–13
Malaysian Palm Oil Statistics. 2003. 23rd ed. Malaysian Palm Oil Board, Bangi, Selangor, Malaysia.
N. Maxted B.V. Ford-Llyod J.G. Hawkes (1997) Plant Genetic Conservation, the in situ Approach Chapman & Hall London
S. Mayes P.L. Jack D.F. Marshall R.H.V. Corley (1997) ArticleTitleConstruction of a RFLP genetic linkage map for oil palm (Elaeis guineensis Jacq.) Genome 40 116–122 Occurrence Handle1:CAS:528:DyaK2sXhvFCks78%3D
S. Mayes C. James S.F. Horner P.L. Jack R.H.V. Corley (1996a) ArticleTitleThe application of restriction fragment length polymorphism for the genetic fingerprinting of oil palm (Elaeis guineensis) Mol. Breed. 2 175–180 Occurrence Handle10.1007/BF00441432 Occurrence Handle1:CAS:528:DyaK28Xlt12qu7c%3D
Mayes S., James C., Price Z., Groves C.M., Jack P.L., Corley and R.H.V. 1996b. Integration of DNA markers into oil palm breeding programmes. In: (eds), Proceeding of 1996 PORIM International Palm Oil Congress – Com-petitiveness for the 21st century (Agriculture). PORIM, Bangi, Selangor, pp. 49–54. n Please suply editors name if possiblen
E. Mayr (1963) Animal Species and Evolution Belknap Press Cambridge
M. Nei (1978) ArticleTitleEstimation of average heterozygosity and genetic distance from a small number of individual Genetics 89 583–590
J.W. Purseglove (1972) Tropical Crops Monocotyledons John Wiley and Sons New York
Rajanaidu N. 1985. The oil palm (Elaeis guineensis) collections in Africa. In: Proceeding of International Workshop on Oil Palm Germplasm and Utilization PORIM, BangiSelangorMarch 26–27, 1985. PORIM, BangiSelangorpp. 59–83.
N. Rajanaidu (1986) ArticleTitleCollection of oil palm (Elaeis guineensis) genetic materials in Tanzania and Madagascar ISOPB Newsletter 3 IssueID4 2–6
Rajanaidu N. 1994a. MPOB Series No.1-MPOB Elite Oil Palm. MPOB Information Series No. 15. MPOB Bangi Selangor.
Rajanaidu N. 1994b. MPOB Series 2-MPOB Elite Oil Palm MPOB Information Series No 16. MPOB Bangi Selangor.
N. Rajanaidu (1995) ArticleTitlePORIM oil palm genebank Palm Oil Tech. Bull. 1 IssueID4 7
N. Rajanaidu (1996) PORIM Elite Oil Palm Series 3 (Mother Palm)- High Kernel. PORIM Information Series No. 41 MPOB Bangi, Selangor
N. Rajanaidu A. Kushairi M. Rafii A. Mohd Din I. Maizura B.S. Jalani (2000) Oil palm breeding and genetic resources B. Yusof B.S. Jalani K.W. Chan (Eds) Advances in Oil Palm Research Malaysian Palm Oil Board Kuala Lumpur 171–237
A.R. Rees (1965) ArticleTitleEvidence for the African Origin of the oil palm Principles 9 30
Sparnaaij L.D. 1958. Oil palm breeding and selection in Belgian Congo. W.A.I.F.O.R. mimeographed report.
Syed R.A. 1980. Pollinating insects of oil palm. CIBC 1977–1980 Report.
N.W. Uhl D. John (1987) Genera Plantaruma Classification of Palms Based on the Work of Harold E. Moore Allen Press LawrenceKansas
Wickneswari R., Norwati M., Siti Salwana H., Nur Supardi M.N. and Aminuddin M. 1997. Seed sources evaluation in Calamus manan Miq. using isozyme markers. Paper presented at EEC-STD3 steering committee – Rattan project FRIM, 15–17 August 1997. Kuala Lumpur.
A.C. Zeven (1964) ArticleTitleOn origin of the oil palm Grana Palynol. 5 50
Zeven A.C. 1967. The semi-wild oil palm and its industry in Africa. Agr. Res. Rpts. No. 689.
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I., M., Rajanaidu, N., Zakri, A.H. et al. Assessment of Genetic Diversity in Oil Palm (Elaeis guineensis Jacq.) using Restriction Fragment Length Polymorphism (RFLP). Genet Resour Crop Evol 53, 187–195 (2006). https://doi.org/10.1007/s10722-004-4004-0
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DOI: https://doi.org/10.1007/s10722-004-4004-0