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Determining Sources of Diversity in Cultivated and Wild Lablab purpureus Related to Provenance of Germplasm by using Amplified Fragment Length Polymorphism

Genetic Resources and Crop Evolution volume 52, pages 683–695 (2005)Cite this article

Abstract

To improve understanding of diversity of Lablab purpureus and establish relationships among 103 germplasm accessions collected from diverse geographic origins, amplified fragment length polymorphism markers were used. Four primer sets selected out of 16 produced 289 clear, repeatable polymorphisms. UPGMA analysis of similarity data clustered the accessions according to their subspecific taxonomic organization, i.e., subsp. purpureus and subsp. uncinatus, as well as to cultivated and wild forms. The well-represented landraces from Africa and Asia, belonging predominantly to subsp. purpureus, displayed moderate genetic diversity. Wild forms from Africa showed far greater levels of diversity that would justify taxonomic re-assessment of the wild subsp. uncinatus. The molecular analysis identified forms that were collected in the wild in India but were genetically placed intermediate between wild and cultivated forms. As these plant types did not exist among the African accessions, it is suggested that they might represent escapes from early attempts of domestication. These results support the suggested pathway of domestication and distribution of L. purpureus from Africa to Asia. Additional members to a previously published core collection of the species are proposed.

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Authors and Affiliations

  1. Institute for Crop and Animal Production in the Tropics, Georg-August-University, Grisebachstr. 6, D-37077, Göttingen, Germany

    Brigitte L. Maass

  2. International Livestock Research Institute (ILRI), P.O. Box 5689, Addis Ababa, Ethiopia

    Brigitte L. Maass & Jean Hanson

  3. ILRI, P.O. Box 30709, Nairobi, Kenya

    Ramni H. Jamnadass

  4. CSIRO Sustainable Ecosystems, 120 Meiers Road, 4068, Indooroopilly, Qld, Australia

    Bruce C. Pengelly

Authors
  1. Brigitte L. Maass
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  2. Ramni H. Jamnadass
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  3. Jean Hanson
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  4. Bruce C. Pengelly
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Correspondence to Brigitte L. Maass.

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Maass, B., Jamnadass, R., Hanson, J. et al. Determining Sources of Diversity in Cultivated and Wild Lablab purpureus Related to Provenance of Germplasm by using Amplified Fragment Length Polymorphism. Genet Resour Crop Evol 52, 683–695 (2005). https://doi.org/10.1007/s10722-003-6019-3

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  • DOI: https://doi.org/10.1007/s10722-003-6019-3

Keywords

  • AFLP
  • Core collection
  • Crop domestication
  • Genetic diversity
  • Lablab purpureus
  • Molecular markers

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