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Structural analysis and in vitro antitumor effect of polysaccharides from Pholiota adiposa

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Abstract

Pholiota adiposa is an edible chestnut mushroom with many health benefits, such as antioxidant and anticancer activity. In this paper, polysaccharides were extracted from Pholidota adiposa using an acid extraction process. The crude polysaccharide was purified using DEAE-cellulose chromatography, and two polysaccharide fractions of SPAP2-1 and SPAP2-2 were obtained. The structure was characterized using UV, GPC, GC, FT-IR, methylation, and NMR analysis. Monosaccharide component analysis indicated that SPAP2-1 (19 kDa) and SPAP2-2 (20 kDa) contained mannose, glucose, and galactose with different molecular ratios. Their antitumor effects were investigated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium (MTT) assay, Annexin V-fluorescein isothiocyanate (FITC), propidium iodide (PI) staining, and flow cytometry. By analyzing the changes in the cells, SPAP2-1 caused damage and changed the proliferation rate of HeLa cells. SPAP2-1 showed strong interference to the cell cycle of HeLa cells and induced cell apoptosis. Overall, these results suggested that polysaccharides from Pholiota adiposa, especially SPAP2-1, may have the potential to be used as a tumor cell inhibitor, which needs further study.

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References

  1. Zou, Y., Du, F., Hu, Q., Wang, H.: The structural characterization of a polysaccharide exhibiting antitumor effect from Pholiota adiposa mycelia.Scientific Reports. 9. (2019)

  2. Lee, J.W., Park, M.S., Park, J.-H., Cho, Y., Kim, C., Kim, C.S., Jo, J.W., Lim, Y.W.: Taxonomic Study of the Genus Pholiota (Strophariaceae, Basidiomycota) in Korea. Mycobiology. 48, 476–483 (2020)

    Article  Google Scholar 

  3. Jatuwong, K., Kumla, J., Suwannarach, N., Matsui, K., Lumyong, S.: Bioprocessing of Agricultural Residues as Substrates and Optimal Conditions for Phytase Production of Chestnut Mushroom, Pholiota adiposa, in Solid State Fermentation.Journal of Fungi.6 (2020)

  4. Wang, C.R., Qiao, W.T., Zhang, Y.N., Liu, F.: Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines. Molecules. 18, 1775–1782 (2013)

    Article  CAS  Google Scholar 

  5. Deng, P., Zhang, G., Zhou, B., Lin, R., Jia, L., Fan, K., Liu, X., Wang, G., Wang, L., Zhang, J.: Extraction and in vitro antioxidant activity of intracellular polysaccharide by Pholiota adiposa SX-02. J. Bioscience Bioeng. 111, 50–54 (2011)

    Article  CAS  Google Scholar 

  6. Dulger, B.: Antimicrobial activity of the macrofungus Pholiota adiposa. Fitoterapia. 75, 395–397 (2004)

    Article  Google Scholar 

  7. Chul, K.K., Hyoung, L.D., Hoyu, K.J., Yu, H.E., Sik, P.G., Soo, L.J.: Production and characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Pholiota adiposa. J. Microbiol. Biotechnol. 16, 757–763 (2006)

    Google Scholar 

  8. Muk, C.H., Min, L.Y., Hyoung, L.D., Kyung, C.H., Soo, L.J.: Effect of a Pholiota adiposa Extract on Fat Mass in Hyperlipidemic Mice.Mycobiology.34 (2006)

  9. Zhang, G.Q., Sun, J., Wang, H.X., Ng, T.B.: A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposa. Acta Biochim. Pol. 56, 415–421 (2009)

    Article  CAS  Google Scholar 

  10. Yu, H.E., Lee, D.H., Seo, G.S., Cho, S.M., Lee, J.S.: Characterization of a novel beta-hydroxy-beta-methyl glutaryl coenzyme a reductase-inhibitor from the mushroom, Pholiota adiposa. Biotechnol. Bioprocess Eng. 12, 618–624 (2007)

    Article  CAS  Google Scholar 

  11. Wang, C.R., Zhou, R., Ng, T.B., Wong, J.H., Qiao, W.T., Liu, F.: First report on isolation of methyl gallate with antioxidant, anti-HIV-1 and HIV-1 enzyme inhibitory activities from a mushroom (Pholiota adiposa). Environ. Toxicol. Pharmacol. 37, 626–637 (2014)

    Article  CAS  Google Scholar 

  12. Yi, Y., Xu, W., Wang, H.X., Huang, F., Wang, L.M.: Natural polysaccharides experience physiochemical and functional changes during preparation: A review.Carbohydrate Polymers.234(2020)

  13. Liu, Z.H., Niu, F.J., Xie, Y.X., Xie, S.M., Liu, Y.N., Yang, Y.Y., Zhou, C.Z., Wan, X.H.: A review: Natural polysaccharides from medicinal plants and microorganisms and their anti-herpetic mechanism.Biomedicine & Pharmacotherapy.129(2020)

  14. Hou, C., Chen, L., Yang, L., Ji, X.: An insight into anti-inflammatory effects of natural polysaccharides. Int. J. Biol. Macromol. 153, 248–255 (2020)

    Article  CAS  Google Scholar 

  15. Mohan, K., Muralisankar, T., Uthayakumar, V., Chandirasekar, R., Revathi, N., Ganesan, A.R., Velmurugan, K., Sathishkumar, P., Jayakumar, R., Seedevi, P.: Trends in the extraction, purification, characterisation and biological activities of polysaccharides from tropical and sub-tropical fruits - A comprehensive review.Carbohydrate Polymers.238 (2020)

  16. Yiemei, J.I., Qingxiu, H.U., Chunyu, G., Guang, G.U.O., Rongshu, A.N.: Antioxidation Study of Extra-polysaccharides Extracted from Pholiota adiposa(Fr.). Quel Biotechnol. 17, 29–31 (2007)

    Google Scholar 

  17. Zhou, C., Sun, Y., Li, D.H., Gu, J.L., Gong, J.H.: Inhibitory Effect of Polysaccharides from Pholiota adiposa on Tumor Cell Proliferation. Mod. Food Sci. Technol. 33, 56–62 (2017)

    CAS  Google Scholar 

  18. Zhao, J.-L., Zhang, M., Zhou, H.-L.: Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng.Molecules.24 (2019)

  19. Yuan, S., Xu, C.-Y., Xia, J., Feng, Y.-N., Zhang, X.-F., Yan, Y.-Y.: Extraction of polysaccharides from Codonopsis pilosula by fermentation with response surface methodology. Food Sci. Nutr. 8, 6660–6669 (2020)

    Article  CAS  Google Scholar 

  20. Yuan, J., Yan, X., Chen, X., Jiang, X., Ye, K., Xiong, Q., Kong, J., Huang, Y., Jiang, C., Xu, T., Xie, G.: A mild and efficient extraction method for polysaccharides from Sinonovacula constricta and study of their structural characteristic and antioxidant activities. Int. J. Biol. Macromol. 143, 913–921 (2020)

    Article  CAS  Google Scholar 

  21. Wang, Y., Wang, C., Guo, M.: Effects of Ultrasound Treatment on Extraction and Rheological Properties of Polysaccharides from Auricularia Cornea var.Li. Molecules.24 (2019)

  22. Li, Y., Qin, G., Cheng, C., Yuan, B., Huang, D., Cheng, S., Cao, C., Chen, G.: Purification, characterization and anti-tumor activities of polysaccharides from Ecklonia kurome obtained by three different extraction methods. Int. J. Biol. Macromol. 150, 1000–1010 (2020)

    Article  CAS  Google Scholar 

  23. Xu, J., Tan, Z.-C., Shen, Z.-Y., Shen, X.-J., Tang, S.-M.: Cordyceps cicadae polysaccharides inhibit human cervical cancer hela cells proliferation via apoptosis and cell cycle arrest.Food and Chemical Toxicology.148 (2021)

  24. Yang, J., Chanok, L., Heekyu, K., Kwon, Y.S., Kim, M.J.: Biological Activities and Phenolic Compound Content of Astilboides tabularis (Hemsl.) Engler Extracts. Curr. Pharm. Biotechnol. 21, 1070–1078 (2020)

    Article  CAS  Google Scholar 

  25. Chai, Y., Zhao, M.: Purification, characterization and anti-proliferation activities of polysaccharides extracted from Viscum coloratum (Kom.) Nakai. Carbohydr. Polym. 149, 121–130 (2016)

    Article  CAS  Google Scholar 

  26. El-Naggar, N.E.-A., Hussein, M.H., Shaaban-Dessuuki, S.A., Dalal, S.R.: Production, extraction and characterization of Chlorella vulgaris soluble polysaccharides and their applications in AgNPs biosynthesis and biostimulation of plant growth.Scientific Reports.10 (2020)

  27. Wang, Z., Luo, D., Liang, Z.: Structure of polysaccharides from the fruiting body of Hericium erinaceus Pers.Carbohydrate Polymers.57(2004)

  28. Kozarski, M., Klaus, A., Niksic, M., Jakovljevic, D., Helsper, J.P.F.G., Van Griensven, L.J.L.D.: Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. Food Chem. 129, 1667–1675 (2011)

    Article  CAS  Google Scholar 

  29. Zeng, W.-C., Zhang, Z., Gao, H., Jia, L.-R., Chen, W.-Y.: Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction.Carbohydrate Polymers.89 (2012)

  30. Zhao, G.H., Kan, J.Q., Li, Z.X., Chen, Z.D.: Structural features and immunological activity of a polysaccharide from Dioscorea opposita Thunb roots. Carbohydr. Polym. 61, 125–131 (2005)

    Article  CAS  Google Scholar 

  31. Parker, F.S.: Application of IR Sepectroscopy in Biochemistry, Biology, and Medicine. Plenum, New York (1971)

    Google Scholar 

  32. Zhu, Y., Li, Q., Mao, G., Zou, Y., Feng, W., Zheng, D., Wang, W., Zhou, L., Zhang, T., Yang, J., Yang, L., Wu, X.: Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus. Carbohydr. Polym. 101, 606–613 (2014)

    Article  CAS  Google Scholar 

  33. Yang, W., Pei, F., Shi, Y., Zhao, L., Fang, Y., Hu, Q.: Purification, characterization and anti-proliferation activity of polysaccharides from Flammulina velutipes. Carbohydr. Polym. 88, 474–480 (2012)

    Article  CAS  Google Scholar 

  34. Guo, L., Xie, J., Ruan, Y., Zhou, L., Zhu, H., Yun, X., Jiang, Y., Lue, L., Chen, K., Min, Z., Wen, Y., Gu, J.: Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum. Int. Immunopharmacol. 9, 1175–1182 (2009)

    Article  CAS  Google Scholar 

  35. Khaskheli, S.G., Zheng, W., Sheikh, S.A., Khaskheli, A.A., Liu, Y., Soomro, A.H., Feng, X., Sauer, M.B., Wang, Y.F., Huang, W.: Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product. Int. J. Biol. Macromol. 81, 387–395 (2015)

    Article  CAS  Google Scholar 

  36. Li, S.-G., Wang, D.-G., Tian, W., Wang, X.-X., Zhao, J.-X., Liu, Z., Chen, R.: Characterization and anti-tumor activity of a polysaccharide from Hedysarum polybotrys Hand.-Mazz. Carbohydr. Polym. 73, 344–350 (2008)

    Article  CAS  Google Scholar 

  37. Li, B., Dobruchowska, J.M., Gerwig, G.J., Dijkhuizen, L., Kamerling, J.P.: Structural investigation of water-soluble polysaccharides extracted from the fruit bodies of Coprinus comatus. Carbohydr. Polym. 91, 314–321 (2013)

    Article  CAS  Google Scholar 

  38. Zhang, H., Li, W.-J., Nie, S.P., Chen, Y., Wang, Y.X., Xie, M.Y.: Structural characterisation of a novel bioactive polysaccharide from Ganoderma atrum. Carbohydr. Polym. 88, 1047–1054 (2012)

    Article  CAS  Google Scholar 

  39. Li, S.-G., Zhang, Y.Q.: Characterization and renal protective effect of a polysaccharide from Astragalus membranaceus. Carbohydr. Polym. 78, 343–348 (2009)

    Article  CAS  Google Scholar 

  40. Gonzaga, M.L.C., Ricardo, N., Heatley, F., Soares, S.D.: Isolation and characterization of polysaccharides from Agaricus blazei Murill. Carbohydr. Polym. 60, 43–49 (2005)

    Article  CAS  Google Scholar 

  41. Kawagishi, H.: Formolysis of a potent antitumor (1 → 6)-β-d-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products. Carbohydr. Polym. 12, 393–403 (1990)

    Article  CAS  Google Scholar 

  42. Liu, C., Sun, Y., Mao, Q., Guo, X., Li, P., Liu, Y., Xu, N.: Characteristics and Antitumor Activity of Morchella esculenta Polysaccharide Extracted by Pulsed Electric Field.International Journal of Molecular Sciences.17 (2016)

  43. Mei, Y., Zhu, H., Hu, Q., Liu, Y., Zhao, S., Peng, N., Liang, Y.: A novel polysaccharide from mycelia of cultured Phellinus linteus displays antitumor activity through apoptosis. Carbohydr. Polym. 124, 90–97 (2015)

    Article  CAS  Google Scholar 

  44. Ren, D., Jiao, Y., Yang, X., Yuan, L., Guo, J., Zhao, Y.: Antioxidant and antitumor effects of polysaccharides from the fungus Pleurotus abalonus. Chemico-Biol. Interact. 237, 166–174 (2015)

    Article  CAS  Google Scholar 

  45. Bae, J.S., Jang, K.H., Yim, H., Jin, H.K.: Polysaccharides isolated from Phellinus gilvus inhibit.melanoma growth in mice. Cancer Lett. 218, 43–52 (2005)

    Article  CAS  Google Scholar 

  46. Zhang, M., Chen, H., Jin, H., Li, Z., Zhu, C., Zhang, S.: Effect of lycium barbarum polysaccharide on human hepatoma qgy7703 cells: inhibition of proliferation and induction of apoptosis. Life Sci. 76, 2115–2124 (2005)

    Article  CAS  Google Scholar 

  47. Zirk, N.M., Hashmi, S.F., Ziegler, H.K.: The polysaccharide portion of lipopolysaccharide regulates antigen-specific t-cell activation via effects on macrophage-mediated antigen processing. Infect. Immun. 6, 319–326 (1999)

    Article  Google Scholar 

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Acknowledgements

This work was supported by the Hundred Million Science and Technology Major Special Project of Heilongjiang Province (2020ZX07B01-3), Fundamental Research Funds for the Central Universities [2572019BA04], Harbin Science and Technology Bureau Science and Technology Innovation Talent Research Fund [2017RAQXJ091], the Natural Science Foundation of Heilongjiang Province [C2015062], and the China Scholarship Commission.

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Authors and Affiliations

  1. Food science and engineering, School of Forestry, Northeast Forestry University, 150040, Harbin, Heilongjiang, China

    Jiao Zhou, Jinhua Gong, Yangyang Chai, Dehai Li & Cong Zhou

  2. Department of Food Science and Engineering, School of Chemical and Environmental Engineering, Harbin University of Science and Technology, 150040, Harbin, Heilongjiang, China

    Changyan Sun

  3. Department of Biomedical Sciences, City University of Hong Kong, 999077, Hong Kong, China

    Jinhua Gong

  4. Department of Food Science, Cornell University, NY14853-7201, Ithaca, USA

    Joe M. Regenstein

  5. Fujian Bakingdom Foods Co., Ltd, 363000, Zhangzhou, Fujian, China

    Cong Zhou

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  1. Jiao Zhou
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  2. Jinhua Gong
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  4. Dehai Li
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  5. Cong Zhou
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Contributions

Dehai Li designed the experiments; Jiao Zhou supervised the research and drafted the manuscript. Jinhua Gong experiment performance and data analysis; Cong Zhou performed the extraction and purification of polysaccharides. Joe M. carried out the chemical and statistical analyses. Changyan Sun and Yangyang Chai revised the manuscript. All authors read and approved the final article.

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Correspondence to Dehai Li.

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Zhou, J., Gong, J., Chai, Y. et al. Structural analysis and in vitro antitumor effect of polysaccharides from Pholiota adiposa. Glycoconj J 39, 513–523 (2022). https://doi.org/10.1007/s10719-022-10065-9

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