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Isolation, purification and structural characterization of two pectin‐type polysaccharides from Coreopsis tinctoria Nutt. and their proliferation activities on RAW264.7 cells

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Abstract

Coreopsis tinctoria Nutt. (C.tinctoria) is an annual herb of the Compositae family with many health benefits, such as clearing heat, antioxidant and anticancer activity. In this paper, two polysaccharides were isolated from C.tinctoria, named CTAP-1 and CTAP-2, respectively. Structure of CTAP-1and CTAP-2 were elucidated by high-performance gel permeation chromatography, chemical derivative analyses, GC-MS and NMR techniques. Results reveal that they both CTAP-1 and CTAP-2 consisted of predominant amounts of galacturonic acid residues along with small amounts of arabinose, rhamnose and galactose.Both them contain homogalacturonan and rhammnogalcturan I regions in different ratio, suggesting their pectin-type features. The proliferation activities of CTAP-1 and CTAP-2 on RAW264.7 cells in vitro were detected. Results show both them have the significant proliferation effect on RAW264.7 cells when the concentration from 40 to 200 µg/mL. Given their structural characteristics and proliferation activities, the pectins are expected to be potential natural immune modulators, which need further study.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China [81803682] and Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis [CTD2018-03].

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  1. Si-Liang Jiang and Yi-Xuan Li contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmacognosy, College of Pharmacy, Jiamusi University, Jiamusi, 154007, China

    Si-Liang Jiang, Yi-Xuan Li & Juan Du

  2. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China

    Si-Liang Jiang, Yi-Xuan Li, Yong-Sheng Cui & Caixia Dong

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Jiang, SL., Li, YX., Cui, YS. et al. Isolation, purification and structural characterization of two pectin‐type polysaccharides from Coreopsis tinctoria Nutt. and their proliferation activities on RAW264.7 cells. Glycoconj J 38, 251–259 (2021). https://doi.org/10.1007/s10719-021-09982-y

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