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Differences in anti-inflammatory properties of water soluble and insoluble bioactive polysaccharides in lipopolysaccharide-stimulated RAW264.7 macrophages

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Abstract

β-Linked polysaccharides including β-glucans are well known to be important functional ingredients, and are known to possess immunomodulatory and anti-tumor activities. This study aimed to investigate the anti-inflammatory properties and participating receptor of water soluble and insoluble bioactive polysaccharides from Grifola frondosa (GFP, non-digestible water soluble polysaccharides), Laminaria digitata (laminarin, a water soluble β-glucan) and Saccharomyces cerevisiae (zymosan, a water insoluble β-glucan) in lipopolysaccharide (LPS)-stimulated parental and Dectin-1 highly expressing RAW264.7 macrophages. Results showed that GFP and laminarin significantly inhibited nitric oxide and prostaglandin E2 production, but only the GFP with high molecular weight exhibited strong inhibition on pro-inflammatory cytokine (TNF-α and IL-6) secretion in a concentration-dependent manner. The activation of NF-κB was also significantly down-regulated by GFP treatment as compared with cells treated with LPS alone. Although GFP and laminarin were able to bind to β-glucan receptor Dectin-1, there was no relationship between the inhibitory potency and the content of β-glucans in GFP, and these inhibitory effects were not affected by the expression level of Dectin-1 in macrophage cells. In contrast, zymosan significantly intensified LPS-induced inflammatory responses through Dectin-1. In conclusion, these results suggest that the inhibitory effects of water soluble polysaccharides on LPS-induced pro-inflammatory mediator production in murine macrophages may not involve β-glucan receptor Dectin-1.

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Acknowledgments

The authors sincerely thank Prof. Gordon D. Brown and Dr. Janet A. Willment for providing the parental RAW264.7 macrophages and Dectin-1 highly expressing RAW264.7 macrophages, and for their helpful guidance.

Funding

This study was supported by the Ministry of Science and Technology of Taiwan under grant number MOST 108–2313-B-002-045.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan

    Chun-Han Su, Yun-Ting Tseng, Kai-Yin Lo & Lean-Teik Ng

  2. Kang Jian Biotech Co., Ltd., Nantou, 54245, Taiwan

    Ming-Nan Lai

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  1. Chun-Han Su
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Contributions

C.H.S. and L.T.N. designed the study, analyzed the data and wrote the manuscript. C.H.S. performed the experiments. Y.T.T. performed Western blot experiments. K.Y.L. provided guidance on Western blotting and reviewed the related results. M.N.L. provided the mushroom samples and reviewed the study design.

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Correspondence to Lean-Teik Ng.

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Su, CH., Tseng, YT., Lo, KY. et al. Differences in anti-inflammatory properties of water soluble and insoluble bioactive polysaccharides in lipopolysaccharide-stimulated RAW264.7 macrophages. Glycoconj J 37, 565–576 (2020). https://doi.org/10.1007/s10719-020-09934-y

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  • DOI: https://doi.org/10.1007/s10719-020-09934-y

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