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Structural characterization and antioxidant potential of a novel exopolysaccharide produced by Bacillus velezensis SN-1 from spontaneously fermented Da-Jiang

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Abstract

The aim of this study was to characterize the exopolysaccharide (EPS) produced by Bacillus velezensis SN-1 (B. velezensis SN-1) (EPS-SN-1), which was isolated from the fermented Da jiang. The microbe made crude exopolysaccharides EPS-SN-1 was produced throughout the bacterial growth period, and the highest yield (2.7 g/L) was obtained with sucrose as the carbon source. As per high performance liquid chromatography (HPLC), EPS-SN-1 is a heteropolysaccharide consisting of glucose, mannose and fructose, with a high molecular weight of 2.21 × 105 Da. FTIR spectra further indicated the presence of hydroxyl and carbonyl groups, and NMR analysis confirmed both α- and β-glycosidic bonds. Furthermore, differential scanning calorimetry (DSC) showed that EPS-SN-1 has high thermal stability with fusion point of 270.7 °C. Finally, EPS-SN-1 demonstrated strong antioxidant capacity via its ability to scavenge hydroxyl radical (•OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical, ABTS radical (ABTS•+) and oxygen radical (O2−•). Taken together, EPS-SN-1 is a promising natural antioxidant and probiotic with potential applications in the food industry.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No.31972047). Guiding Plan of Natural Science Foundation of Liaoning Province (2019-ZD-0714), Liaoning Revitalization Talents Program (XLYC1807040). Liaoning Support Program for innovative talents (LR2019065), Liaoning BaiQianWan Talents Program Project.

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Authors and Affiliations

  1. College of Food Science, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

    Chengxu Cao, Yang Li, Yan Zhang, Yue Zhao, Rina Wu & Junrui Wu

  2. Foreign Language Teaching Department, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

    Yiming Liu

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  1. Chengxu Cao
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Correspondence to Rina Wu or Junrui Wu.

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Cao, C., Liu, Y., Li, Y. et al. Structural characterization and antioxidant potential of a novel exopolysaccharide produced by Bacillus velezensis SN-1 from spontaneously fermented Da-Jiang. Glycoconj J 37, 307–317 (2020). https://doi.org/10.1007/s10719-020-09923-1

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