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Logically derived sequence tandem mass spectrometry for structural determination of Galactose oligosaccharides

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Abstract

Mass spectrometry has high sensitivity and is widely used in the identification of molecular structures, however, the structural determination of oligosaccharides through mass spectrometry is still challenging. A novel method, namely the logically derived sequence (LODES) tandem mass spectrometry (MSn), for the structural determination of underivatized oligosaccharides was developed. This method, which is based on the dissociation mechanisms, involves sequential low-energy collision-induced dissociation (CID) of sodium ion adducts, a logical sequence for identifying the structurally decisive product ions for subsequent CID, and a specially prepared disaccharide CID spectrum database. In this work, we reported the assignment of the specially prepared galactose disaccharide CID spectra. We used galactose trisaccharides and tetrasaccharides as examples to demonstrate LODES/MSn is a general method that can be used for the structural determination of hexose oligosaccharides. LODES/MSn has the potential to be extended to oligosaccharides containing other monosaccharides provided the dissociation mechanisms are understood and the corresponding disaccharide database is available.

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Acknowledgements

This work was financially supported in part by the Thematic Research Project, (AS-107-TP-A08), Academia Sinica, Taiwan.

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Authors and Affiliations

  1. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei, 10617, Taiwan

    Shih-Pei Huang, Hsu Chen Hsu, Chia Yen Liew, Shang-Ting Tsai & Chi-Kung Ni

  2. Molecular Science and Technology, Taiwan International Graduate Program, Academia Sinica and National Taiwan University, Taipei, 10617, Taiwan

    Chia Yen Liew

  3. Department of Chemistry, National Tsing Hua University, Hsinchu, 30013, Taiwan

    Chi-Kung Ni

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  1. Shih-Pei Huang
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Correspondence to Chi-Kung Ni.

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Huang, SP., Hsu, H.C., Liew, C.Y. et al. Logically derived sequence tandem mass spectrometry for structural determination of Galactose oligosaccharides. Glycoconj J 38, 177–189 (2021). https://doi.org/10.1007/s10719-020-09915-1

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