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Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial

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Abstract

Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by 1H – nuclear magnetic resonance spectroscopy and matrix – assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–3)Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Neu5Ac(α2–3) Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc, Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with an α(2–3)Neu5Ac linked to β(1–4)Gal residue of either branch of Gal(β1–4)GlcNAc(β1–6) units, and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc with a β(1–3) linked Gal and an α(2–3) linked Neu5Ac. In addition, larger oligosaccharides were characterized as follows; Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)Glc and Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–3){Gal(β1–3)[Gal(β1–4)GlcNAc(β1–6)]Gal(β1–4)GlcNAc(β1–6)}Gal(β1–4)Glc and their α(2–3) linked Neu5Ac derivatives.

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Authors and Affiliations

  1. Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture & Veterinary Medicine, Hokkaido, Obihiro, 080-8555, Japan

    Tadasu Urashima, Tomoko Yamamoto, Kentaro Hirayama & Kenji Fukuda

  2. Department of Food Science, Obihiro University of Agriculture & Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan

    Tadashi Nakamura

  3. Graduate School of Agriculture, Tohoku University, Sendai, 981-8555, Japan

    Tadao Saito

  4. 13 Pepper Street,, Magill, SA, 5072, Australia

    Keith Newgrain

  5. 20 Templeton St, Cook, ACT, 2614, Australia

    Jim Merchant

  6. Institute for Applied Ecology, University of Canberra, ACT, Canberra, 2601, Australia

    Brian Green

  7. School of Molecular Biosciences, The University of Sydney, NSW, Sydney, 2006, Australia

    Michael Messer

Authors
  1. Tadasu Urashima
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  2. Tomoko Yamamoto
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  3. Kentaro Hirayama
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  4. Kenji Fukuda
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  5. Tadashi Nakamura
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  6. Tadao Saito
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  7. Keith Newgrain
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  8. Jim Merchant
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  9. Brian Green
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  10. Michael Messer
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Correspondence to Tadasu Urashima.

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Urashima, T., Yamamoto, T., Hirayama, K. et al. Chemical characterization of milk oligosaccharides of the tiger quoll (Dasyurus maculatus), a marsupial. Glycoconj J 33, 797–807 (2016). https://doi.org/10.1007/s10719-016-9675-1

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  • DOI: https://doi.org/10.1007/s10719-016-9675-1

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