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Expression of ST3Gal, ST6Gal, ST6GalNAc and ST8Sia in human hepatic carcinoma cell lines, HepG-2 and SMMC-7721 and normal hepatic cell line, L-02

Glycoconjugate Journal volume 32, pages 39–47 (2015)Cite this article

Abstract

We measured ST3Gal, ST6Gal, ST6GalNAc and ST8Sia expression in human hepatic carcinoma cell lines, HepG-2 and SMMC-7721 and normal hepatic cell line, L-02 to reveal the relationship between hepatic carcinoma cell lines sialyltransferases expression and cell membrane sialic acid sugar chains. Membrane sialic acid sugar chains in L-02, HepG-2 and SMMC-7721 cell lines were measured with lectin microarrays to find expression profiles. Expression of 20 sialyltransferases was measured with DNA microarray. qRT-PCR and Western blot were used to verify DNA microarrays data. Siaα 2-3Galβ1-3[Siaα2-6GalNAc]α-R and Siaα 2-6Gal/GalNAc sugar chains in hepatic carcinoma cell lines, HepG-2 and SMMC-7721 were upregulated, and 7differentially expressed sialyltransferases were captured. ST3Gal-IV and ST6Gal I were overexpressed and ST3Gal-I, ST3Gal-V, ST3Gal-VI, ST6GalNAcII and ST6GalNAcVI were downregulated in HepG-2 and SMMC-7721 cell Lines, compared with control cell line. ST6GalNAc-IV and ST8sia expressions were not detected. Other sialyltransferases were not different among cell lines. Results from qRT-PCR and Western blot were consistent with DNA microarray. Overexpression of ST3Gal-IV and ST6Gal I in HepG-2 and SMMC-7721 cell lines may correlate with upregulation of Siaα 2-3Galβ1-3[Siaα2-6GalNAc]α-R and Siaα 2-6Gal/GalNAc sugar chains on cell membranes.

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Acknowledgments

We are grateful to Professor Qun He for her assistance in this work. This work was supported by a grant from the National Natural Science Foundation of China (awarded to Qun He, Grant No. 20672144). We thank LetPub (www.letpub.com) for their linguistic assistance during the preparation of this manuscript.

Conflict of interest statement

None declared

Funding

This work was supported by a grant from the National Natural Science Foundation of China (awarded to Qun He, Grant No. 20672144).

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Authors and Affiliations

  1. Department of Biochemistry, College of Basic Medicine, Shenyang Medical College, Shenyang, 110034, Liaoning, China

    Yan Zhang

  2. Center of Laboratory Technology, China Medical University, Shenyang, 110001, Liaoning, China

    Wenjin Zhao

  3. Biochip Center, College of Basic Medicine, China Medical University, No.92, Beier Road, Shenyang, Liaoning Province, 110001, China

    Yan Zhang & Yujie Zhao

  4. Key Laboratory of Cell Biology,Ministry of Education, Basic Medical School, China Medical University, No.92, Beier Road, Shenyang, Liaoning Province, 110001, China

    Qun He

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  1. Yan Zhang
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  2. Wenjin Zhao
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Correspondence to Yujie Zhao or Qun He.

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This work was supported by a grant from the National Natural Science Foundation of China (awarded to Qun He, Grant No. 20672144).

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Zhang, Y., Zhao, W., Zhao, Y. et al. Expression of ST3Gal, ST6Gal, ST6GalNAc and ST8Sia in human hepatic carcinoma cell lines, HepG-2 and SMMC-7721 and normal hepatic cell line, L-02. Glycoconj J 32, 39–47 (2015). https://doi.org/10.1007/s10719-014-9569-z

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  • DOI: https://doi.org/10.1007/s10719-014-9569-z

Key words

  • Hepatic carcinoma
  • Sialic acid sugar chain
  • Sialyltransferase
  • Lectin microarray
  • DNA microarray