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Excretion into feces of asialo GM1 in the murine digestive tract and Lactobacillus johnsonii exhibiting binding ability toward asialo GM1. A possible role of epithelial glycolipids in the discharge of intestinal bacteria

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Abstract

In the digestive tract of mice (HR-1, 5 months old, ♀), asialo GM1 (GA1) exhibiting receptor activity toward several intestinal bacteria was preferentially expressed in the small intestine. Also, less than 10% of GA1 in the small intestine was converted into fucosylated and sulfated derivatives, but it was completely converted to fucosyl GA1 (FGA1) in the stomach, cecum and colon. Among the lipid components in these tissues, glycolipids other than Forssman antigen and cholesterol sulfate (CS) were present in the digestive tract contents. However, sulfated GA1, sulfatide and fucosyl GM1 in the gastro-intestinal contents were not present in the cecal and colonic contents, in which the major glycolipids were ceramide monohexoside (CMH), GA1 and FGA1. The total amount of GA1 in the whole contents was 20% of that in the tissues. Thus, glycolipids were stable during the process of digestion, and excreted from the body together with cholesterol and CS. On the other hand, Lactobacillus johnsonii (LJ), whose receptor is GA1, was detected in the cecal and colonic contents on sequential analysis of 16S-ribosomal RNA and TLC-immunostaining of antigenic glycolipids with anti-LJ antiserum. LJ was found to comprise 20% of the total bacteria cultured in the lactobacillus medium under aerobic conditions, and to be present in the cecal and colonic contents, 9.8 × 107 cells versus 37 μg GA1 and 1.4 × 108 cells versus 49 μg GA1, respectively. Thus, GA1 in the contents might facilitate the discharge of intestinal bacteria by becoming attached them to prevent their irregular diffusion.

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Abbreviations

CMH:

ceramide monohexoside

CS:

cholesterol sulfate

18t:0:

phytosphingosine

18d:1:

sphingosine

24h:0:

α-hydroxylignoceric acid

GA1:

asialo GM1

FGA1:

fucosyl GA1

FGM1:

fucosyl GM1

SGA1:

sulfated GA1

LJ:

Lactobacillus johnsonii

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-8502, Japan

    Masao Iwamori & Yuriko Iwamori

  2. Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan

    Shigeki Adachi & Taisei Nomura

Authors
  1. Masao Iwamori
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  2. Yuriko Iwamori
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  3. Shigeki Adachi
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  4. Taisei Nomura
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Correspondence to Masao Iwamori.

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Iwamori, M., Iwamori, Y., Adachi, S. et al. Excretion into feces of asialo GM1 in the murine digestive tract and Lactobacillus johnsonii exhibiting binding ability toward asialo GM1. A possible role of epithelial glycolipids in the discharge of intestinal bacteria. Glycoconj J 28, 21–30 (2011). https://doi.org/10.1007/s10719-010-9320-3

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  • DOI: https://doi.org/10.1007/s10719-010-9320-3

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