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Norwalk virus-like particles bind specifically to A, H and difucosylated Lewis but not to B histo-blood group active glycosphingolipids

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Abstract

Noroviruses and norovirus virus-like particles (VLPs) exhibit strain specific patterns in their binding to ABH and Lewis histo-blood group antigens. In this study we demonstrate for the first time specific binding of Norwalk virus VLPs to type 1 and type 2 chain glycosphingolipids (GSLs) carrying ABH and Lewis antigens. N-succinimidyl-3-tributylstannyl benzoate (ATE) was precursor labeled with 125I and then conjugated to VLPs. The 125I-VLPs were used in GSL thin-layer chromatogram binding assays and displayed binding to H type 1, Lewis b, A type 1, A Lewis b GSLs but no binding to B type 1 or B Lewis b GSLs. For the type 2 chain GSLs the Norwalk VLPs bound to H type 2, Lewis y, A type 2 and A Lewis y. In addition, the VLPs bound to several complex GSLs from blood group O and A, but not from blood group B red blood cells.

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Abbreviations

ATE:

N-succinimidyl-3-tributylstannyl benzoate

BSA:

bovine serum albumin

CBA:

chromatogram binding assay

Cer:

ceramide

cpm:

counts per minute

DMSO:

dimethylsulfoxide

ELISA:

enzyme-linked immunosorbent assay

Fuc:

fucose

FUT :

fucosyltransferase gene

G:

genogroup

Gal:

galactose

GalNAc:

N-acetylgalactosamine

Glc:

glucose

GlcNAc:

N-acetylglucosamine

GSL:

glycosphingolipid

HPLC:

high performance liquid chromatography

HRP:

horseradish peroxidase

Le:

Lewis

NV:

Norwalk virus

NMR:

nuclear magnetic resonance

PAA:

polyacrylamido

PBS:

phosphate buffered saline

RBC:

red blood cell

TLC:

thin layer chromatography

TMB:

3, 3’, 5, 5’-tetramethylbenzidine

VLP:

virus-like particle

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Acknowledgments

The authors would like to thank Dr. Sture Lindegren for initial help with the radioiodination protocol, Dr. N. Ruvoën-Clouet (Nantes, France) for help with the NV VLPs purification and antiserum preparation and Dr. Peter Påhlsson (Linköping, Sweden) for the anti-H type 1 monoclonal antibody. We acknowledge the Swedish NMR centre at Hasselblad laboratory, Göteborg University, for their kind supply of NMR spectrometers. The work was supported by grants from the Swedish Research Council (8266), by Governmental grants (ALF) to the Sahlgrenska University Hospital and the French Region Pays de la Loire (CIMATH).

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Authors and Affiliations

  1. Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, SE-413 45, Sweden

    Jonas Nilsson, Gustaf E. Rydell & Göran Larson

  2. Department of Radiation Physics, Institute of Clinical Sciences, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden

    Jonas Nilsson

  3. INSERM, U892, Université de Nantes, Nantes, France

    Jacques Le Pendu

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  1. Jonas Nilsson
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Correspondence to Jonas Nilsson.

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Nilsson, J., Rydell, G.E., Le Pendu, J. et al. Norwalk virus-like particles bind specifically to A, H and difucosylated Lewis but not to B histo-blood group active glycosphingolipids. Glycoconj J 26, 1171–1180 (2009). https://doi.org/10.1007/s10719-009-9237-x

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