Abstract
Mammalian mannose 6-phosphate receptors (MPR 300 and 46) mediate transport of lysosomal enzymes to lysosomes. Recent studies established that the receptors are conserved throughout vertebrates. Although we purified the mollusc receptors and identified only a lysosomal enzyme receptor protein (LERP) in the Drosophila melanogaster, little is known about their structure and functional roles in the invertebrates. In the present study, we purified the putative receptors from the highly evolved invertebrate, starfish, cloned the cDNA for the MPR 46, and expressed it in mpr(−/−) mouse embryonic fibroblast cells. Structural comparison of starfish receptor sequences with other vertebrate receptors gave valuable information on its extensive structural homology with the vertebrate MPR 46 proteins. The expressed protein efficiently sorts lysosomal enzymes within the cells establishing a functional role for this protein. This first report on the invertebrate MPR 46 further confirms the structural and functional conservation of the receptor not only in the vertebrates but also in the invertebrates.
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Acknowledgments
NSK thanks the Volkswagen stiftung, Germany for a research support (I/78913) together with Prof. Dr. K. von Figura, University of Goettingen, and Prof. Dr. R. Pohlmann, University of Muenster, Germany. Research work on the MPR proteins in the laboratory of NSK is supported by a research grant from Department of Science and Technology SR/SO/BB/60-2003. YSRK thanks Council of Scientific and Industrial Research, New Delhi, India for a research fellowship.
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Yadavalli, S., Nadimpalli, S.K. Mannose-6-phosphate receptors (MPR 300 and 46) from the highly evolved invertebrate Asterias rubens (Echinodermate): biochemical and functional characterization of MPR 46 protein. Glycoconj J 25, 889–901 (2008). https://doi.org/10.1007/s10719-008-9153-5
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DOI: https://doi.org/10.1007/s10719-008-9153-5