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The expression patterns of β1,4 galactosyltransferase I and V mRNAs, and Galβ1-4GlcNAc group in rat gastrocnemius muscles post sciatic nerve injury

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Abstract

Glycosylation is one of the most important post-translational modifications. It is clear that the single step of β1,4-galactosylation is performed by a family of β1,4-galactosyltransferases (β1,4-GalTs), and that each member of this family may play a distinct role in different tissues and cells. β1,4-GalT I and V are involved in the biosynthesis of N-linked oligosaccharides and play roles in sciatic nerve regeneration after sciatic nerve injury. In the present study, the expression of β1,4-galactosyltransferase (β1,4-GalT) I, V mRNAs and Galβ1-4GlcNAc group were examined in rat gastrocnemius muscles after sciatic nerve crush and transection. Real time PCR revealed that β1,4-GalT I and V mRNAs expressed at a high level in normal gastrocnemius muscles and decreased gradually from 6 h, reached the lowest level at 2 weeks, then restored gradually to relatively normal level at 4 weeks after sciatic nerve crush. In contrast, in sciatic nerve transection model, β1,4-GalT I and V mRNAs decreased gradually from 6 h, and remained on a low level at 4 weeks in gastrocnemius muscles after sciatic nerve transection. In situ hybridization indicated that β1,4-GalT I and V mRNAs localized in numerous myocytes and muscle satellite cells under normal conditions and at 4 weeks after sciatic nerve crush, and in a few muscle satellite cells at 4 weeks after sciatic nerve transection. Furthermore, lectin blotting showed that the expression level of the Galβ1–4GlcNAc group decreased from 6 h, reached the lowest level at 2 weeks, and restored to relatively normal level at 4 weeks after sciatic nerve crush. RCA-I lectin histochemistry demonstrated that Galβ1–4GlcNAc group localized in numerous membranes of myocytes and muscle satellite cells in normal and at 4 weeks after sciatic nerve crush, and in a few muscle satellite cells at 2 and 4 weeks after sciatic nerve transection. These results indicated that the expressions of β1,4-GalT I, V mRNAs and Galβ1–4GlcNAc group were involved in the process of denervation and reinnervation, which suggests that β1,4-GalT I, V mRNAs and Galβ1-4GlcNAc group may play an important role in the muscle regeneration.

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Abbreviations

β1,4-GalTs:

β1,4-galactosyltransferases

Glcβ1,4NAc:

galactose-β1,4-N-acetylglucosamine

DIG:

digoxigenin

β2-M:

β2-microglobulin

HRP:

horseradish peroxidase

RCA-I:

Ricinus communis agglutinin-I

RT:

room temperature

RT-PCR:

reverse transcription polymerase chain reaction

SD:

Sprague-Dawley

SDS:

sodium dodecyl sulfate

CBB:

Coomassie Brilliant Blue

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Acknowledgements

This work was supported by National Nature Science Foundation of China (Grant No. 30300099, 30772242, 30770488), Natural Science Foundation of Jiangsu Province (BK2003035, BK2006457).

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Authors and Affiliations

  1. The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Meijuan Yan, Chun Cheng, Fei Ding, Jing Jiang, Lingling Gao & Aiguo Shen

  2. Lab of Aging and Nervous Diseases & Cytoneurobiology Unit, Soochow University, Suzhou, 215123, People’s Republic of China

    Meijuan Yan & Chunlin Xia

  3. Nantong University, 19 Qi-Xiu Road, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Meijuan Yan & Aiguo Shen

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  1. Meijuan Yan
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  2. Chun Cheng
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Correspondence to Meijuan Yan, Chunlin Xia or Aiguo Shen.

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Yan, M., Cheng, C., Ding, F. et al. The expression patterns of β1,4 galactosyltransferase I and V mRNAs, and Galβ1-4GlcNAc group in rat gastrocnemius muscles post sciatic nerve injury. Glycoconj J 25, 685–701 (2008). https://doi.org/10.1007/s10719-008-9129-5

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