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Identification of a novel sugar 5,7-diacetamido-8-amino-3,5,7,8,9-pentadeoxy-d-glycero-d-galacto-non-2-ulosonic acid present in the lipooligosaccharide of Vibrio parahaemolyticus O3:K6

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Abstract

A novel sugar, 5,7-diacetamido-8-amino-3,5,7,8,9-pentadeoxy-d-glycero-d-galacto-non-2-ulosonic acid (NonlA), has been identified as a component of the oligosaccharide (OS) isolated from the lipooligosaccharide (LOS) of the emerging strain of Vibrio parahaemolyticus O3:K6 associated with a global pandemic. In the present study we report the identification and characterization of this novel sugar present in the OS of V. parahaemolyticus O3:K6, using chemical analysis, NMR spectroscopy and mass spectrometry.

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Acknowledgements

We thank Prof. Siddhartha Roy, Director, IICB for his constant encouragement. We are thankful to Dr. R. Mukhopadhyay, Mr. E. Padmanaban and Mr. K. K. Sarkar for technical assistance for NMR and mass spectroscopic studies. KM is thankful to CSIR New Delhi for financial support.

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Authors and Affiliations

  1. Department of Chemistry (Carbohydrate), Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata, 700032, India

    Koushik Mazumder & Asish K. Sen

  2. Complex Carbohydrate Research Centre, 315 River Bend Road, Athens, GA, 30602-4712, USA

    Biswa P. Choudhury

  3. International Centre for Diarrhoeal Disease Research, Mohakhali, Dhaka, 1212, Bangladesh

    G. Balakrish Nair

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  1. Koushik Mazumder
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  2. Biswa P. Choudhury
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  3. G. Balakrish Nair
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  4. Asish K. Sen
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Correspondence to Asish K. Sen.

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Mazumder, K., Choudhury, B.P., Nair, G.B. et al. Identification of a novel sugar 5,7-diacetamido-8-amino-3,5,7,8,9-pentadeoxy-d-glycero-d-galacto-non-2-ulosonic acid present in the lipooligosaccharide of Vibrio parahaemolyticus O3:K6. Glycoconj J 25, 345–354 (2008). https://doi.org/10.1007/s10719-007-9080-x

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