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Glycoconjugate Journal

, Volume 25, Issue 1, pp 27–36 | Cite as

Pathogen specific carbohydrate antigen microarrays: a chip for detection of Salmonella O-antigen specific antibodies

  • Ola BlixtEmail author
  • Julia Hoffmann
  • Stefan Svenson
  • Thomas Norberg
Article

Abstract

A Salmonella O-antigen microarray was developed by covalent coupling of oligosaccharide antigens specific for serogroups Salmonella enterica sv. Paratyphi (group A), Typhimurium (group B) and Enteritidis (group D). Antibodies were correctly detected in sera from patients with culture verified salmonellosis. High serogroup-specificity was seen with the disaccharide antigens. With the larger antigens, containing the backbone sequence Manα1–2Rhaα1–2Gal (MRG), common backbone-specific antibodies (O-antigen 12) were also detected. This is “proof of principle” that pathogen-specific carbohydrate antigen microarrays constitute a novel technology for rapid and specific serological diagnosis in either individual patients or larger sero-epidemiological and vaccine studies.

Keywords

Glycanarray Polysaccharide Oligosaccharide Antibody Salmonellosis 

Notes

Acknowledgements

This work was funded by NIGMS and The Consortium for Functional Glycomics GM62116. The authors acknowledge Dr. Dennis Burton (TSRI) for supplying the human healthy control sera obtained from General Clinical Research Center, Scripps Hospital, La Jolla, CA. Ms. Jenesis Kam for proof reading the manuscript. We also would like to express our gratuity to Reagensia AB, Solna, Sweden for supplying some of the sera used in this study.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Ola Blixt
    • 1
    Email author
  • Julia Hoffmann
    • 1
  • Stefan Svenson
    • 2
  • Thomas Norberg
    • 3
  1. 1.Department of Molecular BiologyGlycan Array Synthesis Core D, Consortium for Functional Glycomics. The Scripps Research InstituteLa JollaUSA
  2. 2.Swedish Institute for Infectious Disease ControlSolnaSweden
  3. 3.Department of Biochemistry and Organic ChemistryUppsala UniversityUppsalaSweden

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