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Pathogen specific carbohydrate antigen microarrays: a chip for detection of Salmonella O-antigen specific antibodies

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Abstract

A Salmonella O-antigen microarray was developed by covalent coupling of oligosaccharide antigens specific for serogroups Salmonella enterica sv. Paratyphi (group A), Typhimurium (group B) and Enteritidis (group D). Antibodies were correctly detected in sera from patients with culture verified salmonellosis. High serogroup-specificity was seen with the disaccharide antigens. With the larger antigens, containing the backbone sequence Manα1–2Rhaα1–2Gal (MRG), common backbone-specific antibodies (O-antigen 12) were also detected. This is “proof of principle” that pathogen-specific carbohydrate antigen microarrays constitute a novel technology for rapid and specific serological diagnosis in either individual patients or larger sero-epidemiological and vaccine studies.

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Acknowledgements

This work was funded by NIGMS and The Consortium for Functional Glycomics GM62116. The authors acknowledge Dr. Dennis Burton (TSRI) for supplying the human healthy control sera obtained from General Clinical Research Center, Scripps Hospital, La Jolla, CA. Ms. Jenesis Kam for proof reading the manuscript. We also would like to express our gratuity to Reagensia AB, Solna, Sweden for supplying some of the sera used in this study.

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Authors and Affiliations

  1. Department of Molecular Biology, Glycan Array Synthesis Core D, Consortium for Functional Glycomics. The Scripps Research Institute, CB 248A 10550 N. Torrey Pines Road, La Jolla, CA, 92037, USA

    Ola Blixt & Julia Hoffmann

  2. Swedish Institute for Infectious Disease Control, 17182, Solna, Sweden

    Stefan Svenson

  3. Department of Biochemistry and Organic Chemistry, Uppsala University, Box 576, 751 23, Uppsala, Sweden

    Thomas Norberg

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  1. Ola Blixt
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  2. Julia Hoffmann
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  3. Stefan Svenson
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  4. Thomas Norberg
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Correspondence to Ola Blixt.

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Blixt, O., Hoffmann, J., Svenson, S. et al. Pathogen specific carbohydrate antigen microarrays: a chip for detection of Salmonella O-antigen specific antibodies. Glycoconj J 25, 27–36 (2008). https://doi.org/10.1007/s10719-007-9045-0

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  • DOI: https://doi.org/10.1007/s10719-007-9045-0

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