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Inhibition of galectin-3 mediated cellular interactions by pectic polysaccharides from dietary sources

Glycoconjugate Journal volume 24, pages 497–507 (2007)Cite this article

Abstract

Pectic polysaccharides from dietary sources such as Decalepis hamiltonii—swallow root (SRPP), Hemidesmus indicus (HPP), Nigella sativa—black cumin (BCPP), Andrographis serpyllifolia—(APP), Zingiber officinale—ginger (GRPP) and, citrus pectin (CPP) were examined for galectin inhibitory activity. Inhibition of (a) galectin-3 of MDA-MB-231 cells induced hemagglutination of red blood cells; (b) galectin-3 mediated interaction between normal/metastatic human buccal cells (NBC)/(MBC) and; (c) invasion of MDA-MB-231 and MBC in the invasive chamber was assessed. Results indicated that SRPP inhibited hemagglutination at Minimum Inhibitory Concentration (MIC) of 1.86 μg ml−1 equivalent of carbohydrate as apposed to those of BCPP (130 μg ml−1), APP (40 μg ml−1), HPP (40 μg ml−1) and CPP (25 μg ml−1). GRPP even at concentration >1–6 mg ml−1 did not inhibit agglutination. Also SRPP showed ∼15 and 2 fold potent anti hemagglutination activity relative to that of galectin-3 specific sugars—galactose (MIC-27.1 μg ml−1) and lactose (MIC-4.16 μg ml−1) respectively. Further, SRPP at 10 μg ml−1 inhibited agglutination of NBC by galectin-3 of MDA-MB-231 cells. Modified swallow root pectic polysaccharide (MSRPP) of 50 kDa retained anti hemagglutination activity (MIC of 1.03 μg ml−1) and inhibited MDA-MB-231 and MBC invasion by 73 and 50% with an IC50 of 136 and 200 μg ml−1 respectively. Both SRPP and MSRPP induced apoptosis up to 80% at 100 μg ml−1 concentration by activating ∼2 and 8 folds of Caspase-3 activity. Sugar composition analysis and its correlation with the galectin inhibitory property indicated that pectic polysaccharides with higher arabinose and galactose content—arabinogalactan inhibited hemagglutination significantly.

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Abbreviations

SRPP:

swallow root pectic polysaccharide

HPP:

Hemidesmus pectic polysaccharide

BCPP:

black cumin pectic polysaccharide

APP:

Andrographis pectic polysaccharide

GRPP:

ginger pectic polysaccharide

CPP:

citrus pectic polysaccharide

NBC:

normal human buccal cells

MBC:

metastatic human buccal cells

MIC:

Minimum Inhibitory Concentration

MSRPP:

modified swallow root pectic polysaccharide

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Acknowledgement

Authors thank Dr. V. Prakash, Director, CFTRI, for his keen interest in the work and encouragement. Authors are also thankful to Dr. S.G. Bhat, Head, Department of Biochemistry and Nutrition and Dr. H.P. Ramesh, Head, Cell Culture Facility for their help. SMD thank Department of Biotechnology, New Delhi, India and Department of Science and Technology, New Delhi, India for financial assistance.

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  1. Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, 570 020, India

    U. V. Sathisha, Smitha Jayaram, M. A. Harish Nayaka & Shylaja M. Dharmesh

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  1. U. V. Sathisha
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  2. Smitha Jayaram
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  3. M. A. Harish Nayaka
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  4. Shylaja M. Dharmesh
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Correspondence to Shylaja M. Dharmesh.

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Sathisha, U.V., Jayaram, S., Harish Nayaka, M.A. et al. Inhibition of galectin-3 mediated cellular interactions by pectic polysaccharides from dietary sources. Glycoconj J 24, 497–507 (2007). https://doi.org/10.1007/s10719-007-9042-3

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  • DOI: https://doi.org/10.1007/s10719-007-9042-3

Keywords

  • Galectin-3
  • Pectic polysaccharide
  • Hemagglutination
  • Anti metastatic activity
  • Arabinogalactan
  • Modified pectic polysaccharide