Abstract
Xylanase inhibitor proteins (XIP) are widely distributed in the plant kingdom, and also exist in rice. However, a systematic bioinformatics analysis of this gene family in rice (OsXIP) has not been conducted to date. In this study, we identified 32 members of the OsXIP gene family and analyzed their physicochemical properties, chromosomal localization, gene structure, protein structure, expression profiles, and interaction networks. Our results indicated that OsXIP genes exhibit an uneven distribution across eight rice chromosomes. These genes generally feature a low number of introns or are intronless, all family members, except for OsXIP20, contain two highly conserved motifs, namely Motif 8 and Motif 9. In addition, it is worth noting that the promoter regions of OsXIP gene family members feature a widespread presence of abscisic acid response elements (ABRE) and gibberellin response elements (GARE-motif and TATC-box). Quantitative Real-time PCR (qRT-PCR) analysis unveiled that the expression of OsXIP genes exhibited higher levels in leaves and roots, with considerable variation in the expression of each gene in these tissues both prior to and following treatments with abscisic acid (ABA) and gibberellin (GA3). Protein interaction studies and microRNA (miRNA) target prediction showed that OsXIP engages with key elements within the hormone-responsive and drought signaling pathways. The qRT-PCR suggested osa-miR2927 as a potential key regulator in the rice responding to drought stress, functioning as tissue-specific and temporally regulation. This study provides a theoretical foundation for further analysis of the functions within the OsXIP gene family.
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This study was financially supported by Department of Education Anhui Natural Science Foundation (KJ2020A0119).
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D.C.W., Y.X. and G.S. conceived and designed research. M.Z., Z.L., J.G., C.Y., A.L., Q.H. and H.Y. conducted experiments. D.C.W. and Y.X. contributed new reagents or analytical tools. M.Z., Z.L. and D.C.W. analyzed data and wrote the manuscript. G.S. edited the manuscript. All authors reviewed and approved the manuscript.
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Zhao, M., Liu, Z., Gan, J. et al. Identification and expression analysis of XIP gene family members in rice. Genetica (2024). https://doi.org/10.1007/s10709-024-00207-2
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DOI: https://doi.org/10.1007/s10709-024-00207-2