Abstract
The genetic diversity and phylogenetic relationships of Oxytropis caespitosa, O. grandiflora, O. eriocarpa, O. mixotriche, O. nitens, O. peschkovae and O. triphylla, section Xerobia subgenus Oxytropis, in one of the main speciation centres of the genus Oxytropis (Baikal Siberia and adjacent territories of Northeastern Mongolia) were studied based on sequence analysis of the psbA–trnH, trnL–trnF and trnS–trnG intergenic spacers of cpDNA, as well as the ITS nrDNA. Most populations are characterized by a high level of chloroplast genetic diversity (h varied from 0.327 to 1.000 and π from 0.0001 to 0.0090) due to the ancient origin for some species and to hybridization and polyploidy for others. 67 haplotypes were identified, of which six were shared. Phylogenetic relationships among species could not be satisfactorily resolved. Only the haplotypes of O. triphylla formed a group with rather high support. Probably, O. caespitosa, O. grandiflora, O. mixotriche and O. nitens constitute a single genetic complex. As regards the ITS nrDNA polymorphism, we detected only two ribotypes (RX1, RX2). Both were found in O. caespitosa, O. eriocarpa, O. mixotriche and O. peschkovae, while RX1 was present in O. nitens and O. triphylla, RX2 in O. grandiflora. The absence of diagnostic species-specific variants for the markers studied, together with the sharing of cpDNA haplotypes and nrDNA ribotypes between species, and the resulting polytomies on the phylogenetic trees, confirm the hypothesis on the hybrid origin of some of them. Obviously, the reproductive barriers within the sect. Xerobia are weak. However, morphological differences between the species of the sect. Xerobia are clearly pronounced, even when they grow in sympatry.
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References
Amini E, Kazempour-Osaloo Sh, Maassoumi AA, Zare-Maivan H (2019) Phylogeny, biogeography and divergence times of Astragalus section Incani DC. (Fabaceae) inferred from nrDNA ITS and plastid rpl32-trnL(UAG) sequences. Nord J Bot. https://doi.org/10.1111/njb.02059
Archambault A, Strömvik MV (2012) Evolutionary relationships in Oxytropis species, as estimated from the nuclear ribosomal internal transcribed spacer (ITS) sequences point to multiple expansions into the Arctic. Botany 90:770–779. https://doi.org/10.1139/B2012-023
Arkad’eva GE, Blinova KF, Komarova MN (1966) K antibioticheskoy otsenke lekarstvennyih rasteniy tibetskoy meditsinyi. Rastitelnyie Resursyi 2:218–223
Artyukova EV, Kholina AB, Kozyrenko MM, Zhuravlev YuN (2004) Analysis of genetic variation in rare endemic species Oxytropis chankaensis Jurtz. (Fabaceae) using RAPD markers. Russ J Genet 40:710–716
Bagheri A, Maassoumi AA, Rahiminejad MR, Brassac J et al (2017) Molecular phylogeny and divergence times of Astragalus section Hymenostegis: an analysis of a rapidly diversifying species group in Fabaceae. Sci Rep 7:14033. https://doi.org/10.1038/s41598-017-14614-3
Bandelt HJ, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48. https://doi.org/10.1093/oxfordjournals.molbev.a026036
Bartha L, Dragos N, Molnar A, Sramko G (2013) Molecular evidence for reticulate speciation in Astragalus (Fabaceae) as revealed by a case study from sect. Dissitiflori. Botany 91:702–714. https://doi.org/10.1139/cjb-2013-0036
Blinova KF, Sakanyan EI (1986) Vidyi Oxytropis DC., primenyaemyie v tibetskoy meditsine, i ih flavonoidnyiy sostav. Rastitelnyie Resursyi 22:266–272
Borchsenius F (2009) FastGap 1.2. University of Aarhus, Aarhus. https://www.aubot.dk/FastGap_home.htm
Dizkirici Tekpinar A, Karaman Erkul S, Aytaç Z, Kaya Z (2016) Phylogenetic relationships between Oxytropis DC. and Astragalus L. species native to an Old World diversity center inferred from nuclear ribosomal ITS and plastid matK gene sequences. Turk J Biol 40:250–263. https://doi.org/10.3906/biy-1502-5
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567
Flora of Baikal Siberia (2010) http://www.flora.baikal.ru
Gouy M, Guindon S, Gascuel O (2010) SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224. https://doi.org/10.1093/molbev/msp259
Grubov VI (1998) Rasteniya Tsentral’noi Azii, vol 8b. Oxytropis DC. Mir i Sem’ya, Sankt-Petersburg
Khalili Z, Ghalenoyi S, Maassoumi AA, Kazempour-Osaloo Sh (2020) Phylogenetic relationships, biogeography and taxonomic delimitation of Astragalus sect. Acanthophace (Fabaceae) using cpDNA and nrDNA ITS sequences analyses. Plant Biosyst. https://doi.org/10.1080/11263504.2020.1739162
Kholina AB, Kozyrenko MM, Artyukova EV, Sandanov DV et al (2016) Phylogenetic relationships of the species of Oxytropis DC. subg. Oxytropis and Phacoxytropis (Fabaceae) from Asian Russia inferred from the nucleotide sequence analysis of the intergenic spacers of the chloroplast genome. Russ J Genet 52:80–793. https://doi.org/10.1134/S1022795416060065
Kholina AB, Kozyrenko MM, Artyukova EV, Sandanov DV (2018a) Modern state of populations of endemic Oxytropis species from Baikal Siberia and their phylogenetic relationships based on chloroplast DNA markers. Russ J Genet 54:805–815. https://doi.org/10.1134/S1022795418070050
Kholina A, Kozyrenko M, Artyukova E, Sandanov D et al (2018b) Plastid DNA variation of the endemic species Oxytropis glandulosa Turcz. (Fabaceae). Turk J Bot 42:38–50. https://doi.org/10.3906/bot-1706-11
Kholina AB, Kozyrenko MM, Artyukova EV, Sandanov DV (2019) The divergence of Oxytropis species of section Verticillares (Fabaceae) of the steppe flora of Baikal Siberia based on chloroplast DNA sequence data. Russ J Genet 55:701–710. https://doi.org/10.1134/S0016675819060055
Kholina AB, Kozyrenko MM, Artyukova EV, Yakubov VV et al (2020) Phylogenetic relationships of Oxytropis section Arctobia of Northeast Asia according to sequencing of the intergenic spacers of the chloroplast and ITS of nuclear genomes. Russ J Genet 56:1424–1434. https://doi.org/10.1134/S1022795420120091
Konichenko ES, Selyutina IY, Dorogina OV (2012) Oxytropis triphylla. In: Marhold K (ed) IAPT/IOPB chromosome data 14. Taxon, Austria
Kozyrenko MM, Kholina AB, Artyukova EV, Koldaeva MN et al (2020) Molecular phylogenetic analysis of the endemic Far Eastern closely related Oxytropis species of section Orobia (Fabaceae). Russ J Genet 56:429–440. https://doi.org/10.31857/S0016675820040049
Krivenko DA, Kotseruba VV, Kazanovsky SG, Verkhozina AV et al (2011) Oxytropis triphylla. In: Marhold K (ed) IAPT/IOPB chromosome data 11. Taxon, Austria
Krivenko DA, Kazanovsky SG, Verkhozina AV, Chernova OD et al (2013) Oxytropisgrandiflora. In: Marhold K (ed) IAPT/IOPB chromosome data 15. Taxon, Austria. https://doi.org/10.12705/625.16
Krivenko DA, Kazanovsky SG, Vinogradova YK, Verkhozina AV et al (2017a) Oxytropis caespitosa. In: Marhold K (ed) IAPT/IOPB chromosome data 26. Taxon, Austria. https://doi.org/10.12705/666.30
Krivenko DA, Kazanovsky SG, Vinogradova YK, Verkhozina AV et al (2017b) Oxytropis peschkovae. In: Marhold K (ed) IAPT/IOPB chromosome data 26. Taxon, Austria. https://doi.org/10.12705/666.30
Lavin M, Herendeen PS, Wojciechowski MF (2005) Evolutionary rates analysis of Leguminosae implicates a rapid diversification of lineages during the Tertiary. Syst Biol 54:530–549. https://doi.org/10.1080/10635150590947131
Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452. https://doi.org/10.1093/bioinformatics/btp187
Ma S, Zhang M (2012) Phylogeography and conservation genetics of the relic Gymnocarpos przewalskii (Caryophyllaceae) restricted to northwestern China. Conserv Genet 13:1531–1541. https://doi.org/10.1007/s10592-012-0397-z
Malyshev LI (2008) Diversity of the genus Oxytropis in the Asian part of Russia. Turczaninowia 11:5–141
Malyshev LI, Peshkova GA (1984) Osobennosti i genezis floryi Sibiri (Predbaykal’e i Zabaykal’e). Nauka, Novosibirsk
Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE). IEEE, New Orleans
Namzalov BB (2009) Baikal phytogeographic node as the newest center of endemism of Inner Asia. Contemp Probl Ecol 2:341–347. https://doi.org/10.1134/S1995425509040079
Peirson JA, Dick CW, Reznicek AA (2013) Phylogeography and polyploid evolution of North American goldenrods (Solidago subsect. Humiles, Asteraceae). J Biogeogr 40:1887–1898. https://doi.org/10.1111/jbi.12136
Peshkova GA (2001) Florogeneticheskiy analiz stepnoy floryi gor Yuzhnoy Sibiri. Nauka, Novosibirsk
Plenk K, Bardy K, Höhn M, Thiv M et al (2017) No obvious genetic erosion, but evident relict status at the westernmost range edge of the Pontic-Pannonian steppe plant Linum flavum L. (Linaceae) in Central Europe. Ecol Evol 7:6527–6539. https://doi.org/10.1002/ece3.2990
Plenk K, Willner W, Demina ON, Höhn M et al (2020) Phylogeographic evidence for long-term persistence of the Eurasian steppe plant Astragalus onobrychisin the Pannonian region (eastern Central Europe). Flora 264:151555. https://doi.org/10.1016/j.flora.2020.151555
Polozhii AV (1965) Florogeneticheskiy analiz ostrolodochnikov Srednei Sibiri. Uchenye Zapiski Tomskogo gosudarstvennogo universiteta. Biologiya i pochvovedenie 51:18–38
Polozhii AV (1994) OxytropisDC. In: Malyshev LI (ed) Flora Sibiri. Vol. 9. Fabaceae (Leguminosae). Nauka, Novosibirsk, pp 74–151
Polozhii AV (2003) On the problem of the origin and evolution of the genus Oxytropis (Fabaceae). Bot Zhurn 88:55–59
Popov MG (1956) Endemism vo flore poberezhii Baikala i ego proiskhozhdenie. In: Sochava VB (ed) Akademiku V.N. Sukachevu k 75-letiyu so dnya rozhdeniya. Izd. AN SSSR, Leningrad, pp 442–462
Popov MG (1957) Oxytropis DC.—Ostrolodka. In: Popov MG (ed) Flora Sredney Sibiri, vol 1. Izd. AN SSSR, Leningrad, pp 336–352
Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818. https://doi.org/10.1093/bioinformatics/14.9.817
Povyidyish MN, Bobyileva NS, Bityukova NV (2010) Oxytropis DC. In: Budantsev AL (ed) Rastitelnyie resursyi Rossii, vol 3. KMK, Sankt-Petersburg, pp 65–69
Pyak AI (2014) Oxytropis sobolevskajae sp. nov. (Fabaceae: Papilionoideae, Galegeae) from Tuva Republic (South Siberia, Russia). Nord J Bot 32:139–142. https://doi.org/10.1111/j.1756-1051.2013.00196.x
Red Book of the Russian Federation (Plants and Fungi) (2008) KMK Scientific Press, Moscow. (in Russian)
Ronquist F, Huelsenbeck JP (2003) MrBAYES3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Segatto ALA, Reck-Kortmann M, Turchetto C, Freitas LB (2017) Multiple markers, niche modelling, and bioregions analyses to evaluate the genetic diversity of a plant species complex. BMC Evol Biol 17:234. https://doi.org/10.1186/s12862-017-1084-y
Shavvon RS, Kazempour-Osaloo S, Maassoumi AA et al (2017) Increasing phylogenetic support for explosively radiating taxa: the promise of high-throughput sequencing for Oxytropis (Fabaceae). J Syst Evol 55:385–404. https://doi.org/10.1111/jse.12269
Shepherd LD, Lange PJ, Perrie LR, Heenan PB (2017) Chloroplast phylogeography of New Zealand Sophora trees (Fabaceae): extensive hybridization and widespread Last Glacial Maximum survival. J Biogeogr 44:1640–1651. https://doi.org/10.1111/jbi.12963
Simmons MP, Ochoterena H (2000) Gaps as characters in sequence-based phylogenetic analyses. Syst Biol 49:369–381. https://doi.org/10.1093/sysbio/49.2.369
Swofford DL (2003) PAUP*: Phylogenetic analysis using parsimony (*and other methods): version 4.04. Sinauer Associates Inc., Sunderland
Tekpinar A, Karaman Erkul S, Aytaç Z, Kaya Z (2016) Phylogenetic relationships among native Oxytropis species in Turkey using trnL intron, trnL–F IGS, and trnV intron cpDNA regions. Turk J Bot 40:472–479. https://doi.org/10.3906/bot-1506-45
Tulokhonov AK (ed) (2009) Baykal: priroda i lyudi. Entsiklopedicheskiy spravochnik. ECOS, Ulan-Ude
Ulziihutag N (2003) Ostrolodochnik—Ortuuz—OxytropisDC. Bobovye Mongolii (taksonomiya, ekologiya, geografiya, filogeniya i khozyaystvennoye znacheniye). Bembi San, Ulaanbaatar, pp 210–282
Weiss-Schneeweiss H, Emadzade K, Jang T-S, Schneeweiss GM (2013) Evolutionary consequences, constraints and potential of polyploidy in plants. Cytogenet Genom Res 140:137–150. https://doi.org/10.1159/000351727
Xu Z, Zhang M-L, Cohen JI (2016) Phylogeographic history of Atraphaxis plants in arid Northern China and the origin of A. bracteata in the Loess Plateau. PLoS ONE 11(9):e0163243. https://doi.org/10.1371/journal.pone.0163243
Zhu X, Welsh SL, Ohashi H (2010) Oxytropis. In: Zhengyi W, Raven PH, Deyuan H (eds) Flora of China, vol 10. Science Press, Beijing, pp 453–500
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Kholina, A., Kozyrenko, M., Artyukova, E. et al. Genetic diversity of Oxytropis section Xerobia (Fabaceae) in one of the centres of speciation. Genetica 149, 89–101 (2021). https://doi.org/10.1007/s10709-021-00115-9
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DOI: https://doi.org/10.1007/s10709-021-00115-9