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Identification and expression profiling of selected MADS-box family genes in Dendrobium officinale

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Abstract

Dendrobium officinale, a herb with highly medicinal and ornamental value, is widely distributed in China. MADS-box genes encode transcription factors that regulate various growth and developmental processes in plants, particular in flowering. However, the MADS-box genes in D. officinale are largely unknown. In our study, expression profiling analyses of selected MADS-box genes in D. officinale were performed. In total, 16 DnMADS-box genes with full-length ORF were identified and named according to their phylogenetic relationships with model plants. The transient expression of eight selected MADS-box genes in the epidermal cells of tobacco leaves showed that these DnMADS-box proteins localized to the nucleus. Tissue-specific expression analysis pointed out eight flower-specific expressed MADS-box genes in D. officinale. Furthermore, expression patterns of DnMADS-box genes were investigated during the floral transition process. DnMADS3, DnMADS8 and DnMADS22 were significantly up-regulated in the reproductive phase compared with the vegetative phase, suggesting putative roles of these DnMADS-box genes in flowering. Our data showed that the expressions of MADS-box genes in D. officinale were controlled by diverse exogenous phytohormones. Together, these findings will facilitate further studies of MADS-box genes in Orchids and broaden our understanding of the genetics of flowering.

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Acknowledgements

This study was supported by the Natural Science Foundation of China (31801891), the Tree Breeding Project of Zhejiang Province, China (2016C02065), the Natural Science Foundation of Zhejiang Province, China (LQ17C150002), and the Open Research Subject of Key Laboratory of Plant Germplasm Enhancement of Specially Forestry of Central and South of Zhejiang Province (ZX201902).

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  1. Yue Chen and Qi Shen have contributed equally to this work.

Authors and Affiliations

  1. Institute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, People’s Republic of China

    Yue Chen & Chongbo Sun

  2. Key laboratory of creative Agriculture, Ministry of Agriculture, Hangzhou, People’s Republic of China

    Yue Chen & Chongbo Sun

  3. Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang, People’s Republic of China

    Qi Shen

  4. Lin’an Agricultural & Forestry Technology Extension Center, Hangzhou, Zhejiang, People’s Republic of China

    Ping Lyu

  5. Yueqing Forestry Varieties Tech Center, Yueqing, Zhejiang, People’s Republic of China

    Renan Lin

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  1. Yue Chen
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Conceived and designed the experiments: YC and QS; Performed the experiments: YC, PL, RL; Analyzed the data: RL; Drafted the manuscript: YC and CBS.

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Correspondence to Chongbo Sun.

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Chen, Y., Shen, Q., Lyu, P. et al. Identification and expression profiling of selected MADS-box family genes in Dendrobium officinale. Genetica 147, 303–313 (2019). https://doi.org/10.1007/s10709-019-00071-5

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