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, Volume 147, Issue 3–4, pp 269–280 | Cite as

Molecular-genetic diversity of the endangered Dalmatian barbelgudgeon, Aulopyge huegelii from the Buško Blato reservoir

  • Belma Kalamujić StroilEmail author
  • Aldijana Mušović
  • Rifat Škrijelj
  • Semir Dorić
  • Samir Đug
  • Naris Pojskić
Original Paper
  • 77 Downloads

Abstract

A number of studies investigating different aspects of IUCN endangered species, Aulopyge huegelii Heckel, 1843 (Dalmatian barbelgudgeon) biology have been conducted, but data on molecular genetics are lacking. The goal of this survey was to assess the genetic structure of the A. huegelii population from the Buško Blato reservoir, based on four mitochondrial DNA regions and five microsatellite loci. Excluding cytochrome b, more than one haplotype has been detected in all sequenced mtDNA regions, most of which had not been previously described. A total of seven composite haplotypes were detected. Nucleotide diversity was relatively low for all coding genes but slightly higher for the control region. Microsatellite analysis revealed a relatively high value of major allele frequency, lower values of observed and expected heterozygosity, as well as a moderately reduced number of alleles and genotypes in three of the five observed loci. Although with a clear trend of decline, the level of genetic diversity is still sufficient to ensure the subsistence of the population if the stressors are removed. Otherwise, the loss of heterozygosity will continue, possibly to the point of a complete eradication of the Dalmatian barbelgudgeon from the Buško Blato reservoir.

Keywords

Dalmatian barbelgudgeon Microsatellite Mitochondrial DNA Reservoir Stressor 

Notes

Acknowledgements

The authors would like to thank Adem Hamzić, Senad Šljuka, Mahir Gajević and Adi Vesnić for their help on the field, as well as Lejla Lasić and Jasna Hanjalić for their help in the laboratory. We kindly thank the Ichthyology and Fishing Center of the Faculty of Science, Sarajevo for the financial support.

Authors’ contributions

BKS and AM conducted the molecular analyses and drafted the paper, while RŠ was the project coordinator who organized the field survey. SD performed all sequential analysis and SĐ created all graphical outputs. NP did the statistical analyses as well as the interpretation of the obtained results. All authors critically read and corrected the manuscript and aproved its final content.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.University of Sarajevo-Institute for Genetic Engineering and BiotechnologySarajevoBosnia and Herzegovina
  2. 2.Faculty of ScienceUniversity of SarajevoSarajevoBosnia and Herzegovina

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