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Genetica

, Volume 147, Issue 2, pp 197–203 | Cite as

Complementary gene interaction and xenia effect controls the seed coat colour in interspecific cross between Trifolium alexandrinum and T. apertum

  • D. R. MalaviyaEmail author
  • A. K. Roy
  • P. Kaushal
  • A. Yadav
  • D. K. Pandey
Original Paper
  • 76 Downloads

Abstract

Trifolium alexandrinum (Egyptian clover) is a widely cultivated winter annual fodder. Present work deals with inheritance of the seed coat colour in segregating progenies of the interspecific cross between T. alexandrinum and T. apertum. Although, both the parent species possessed yellow seed coat, the F1 seeds were black coloured in the reciprocal cross (T. apertum × T. alexandrinum). Seeds borne on individual F2 plants and the advancing generations segregated in yellow and black seed coat colour, which confirmed xenia effect. F2 seeds collected from individual F1 plants exhibited nine black and seven yellow segregation ratio. The segregation of the seed coat colour recorded from F3 to F5 generations revealed that yellow seed coat was true breeding (i.e. non-segregating) in this interspecific cross (including the reciprocal crosses). However, the black seeded progenies were either true breeding or segregated in nine black: seven yellow ratio or three black: one yellow ratio suggesting a complementary gene interaction or duplicate recessive epistasis. It indicated that the seed coat colour is controlled by complementary gene interaction along with xenia effect in interspecific crosses between T. alexandrinum and T. apertum. Occurrence of the complementary genes across the species could suggest T. apertum to be the progenitor of T. alexandrinum. Inheritance of seed coat colour in reference to its importance in Egyptian clover breeding is also discussed.

Keywords

Genetics Interspecific cross Seed colour Trifolium alexandrinum 

Notes

Acknowledgements

Authors are grateful to Director, Indian Grassland and Fodder Research Institute, Jhansi, India for providing facilities and encouragement. Authors are also thankful to Indian Council of Agricultural Research, India for financial support.

Author contributions

DRM contributed in conceptualization, conducting experiment, data recording and analysis, manuscript writing; AKR and PK contributed in conducting experiment, data analysis, manuscript writing; AY conducted experiment and recorded data. DKP helped in data analysis and manuscript writing.

Funding

This study was funded by Indian Council of Agricultural Research, India.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Aaronsohn A (1910) Agricultural explorations in Palestine. USDA Bur Pal Ind Bull, p 180Google Scholar
  2. Atis I, Atak M, Can E, Mavi K (2011) Seed coat colour effects on seed quality and salt tolerance of red clover (Trifolium pratense). Int J Agric Biol 13:363–368Google Scholar
  3. Badr A, El-Shazly HH, Watson LE (2008) Origin and ancestry of Egyptian clover (Trifolium alexandrinum L.) as revealed by AFLP markers. Genet Resour Crop Evol 55:21–31CrossRefGoogle Scholar
  4. Bell JM, Shires A (1982) Composition and digestibility by pigs of hull fractions from rapeseed cultivars with yellow or brown seed coats. Can J Anim Sci 62:557–565CrossRefGoogle Scholar
  5. Bhaskar RB, Malaviya DR, Roy AK, Kaushal P (2002) Evaluation of exotic Trifolium accessions for disease incidence and resistance. In: Abstr. Nat. symp on grassland and fodder research in the new millennium, held at IGFRI, Jhansi. Range Management Society of India, Jhansi, pp 31–32Google Scholar
  6. Bobrov EG (1947) Vidi Kleverov URSS (in Russian). Acta Inst Bot N Acad Sci USSR Ser 1:164–336Google Scholar
  7. Bortnem R, Boe A (2003) Colour index for red clover seed. Crop Sci 43:2279–2283CrossRefGoogle Scholar
  8. Caldas GV, Blair MW (2009) Inheritance of seed condensed tannins and their relationship with seed-coat color and pattern genes in common bean (Phaseolus vulgaris L.). Theor Appl Genet 119:131–142.  https://doi.org/10.1007/s00122-009-1023-4 CrossRefGoogle Scholar
  9. Cardador MA, Castano TE, Loarca PG (2002) Antimutagenic activity of natural phenolic compounds present in common bean (Phaseolus vulgaris) against aflatoxin B1. Food Addit Contam 19:62–69CrossRefGoogle Scholar
  10. Chen BY, Heneen WK (1992) Inheritance of seed color in Brassica campestris L. and breeding for yellow-seeded B. napus L. Euphytica 59:157–163CrossRefGoogle Scholar
  11. Denney JO (1992) Xenia includes metaxenia. Hortscience 27:722–728CrossRefGoogle Scholar
  12. Dooner HK, Robbins TR, Jorgensen RA (1991) Genetic and developmental control of anthocyanin biosynthesis. Annu Rev Genet 25:173–199CrossRefGoogle Scholar
  13. Dueñas M, Estrella I, Hernandez T (2004) Occurrence of phenolic compounds in the seed coat and the cotyledon of peas (Pisum sativum L.). Eur Food Res Technol 219:116–123CrossRefGoogle Scholar
  14. Dymond JR (1921) Colour characteristics of red clover seed. In: Proc. association of official seed analysts of North America, Vol.12/13 (JULY 1921). Association of Official Seed Analysts and the Society of Commercial Seed Technologists (SCST). pp. 30–31Google Scholar
  15. Ellison NW, Liston A, Steiner JJ, Williams WM, Taylor NL (2006) Molecular phylogenetics of the clover genus (Trifolium–Leguminosae). Mol Phylogenet Evol 39:688–705CrossRefGoogle Scholar
  16. Evans AM (1976) Clovers. In: Simmonds NW (ed) Evolution of crop plants. Longman, LondonGoogle Scholar
  17. Heneen WK, Jørgensen RB (2001) Cytology, RAPD, and seed color of progeny plants from Brassica rapa-alboglabra aneuploids and development of monosomic addition lines. Genome 44:1007–1021CrossRefGoogle Scholar
  18. Kaur A, Kaur KP, Kalia A, Rani U, Kahlon JG, Sharma R, Malaviya D, Kapoor R, Sandhu JS (2017) Generation of interspecific hybrids between Trifolium vesiculosum and T. alexandrinum using embryo rescue. Euphytica 213:253.  https://doi.org/10.1007/s10681-017-2042-x CrossRefGoogle Scholar
  19. Kaushal P, Malaviya DR, Roy AK, Kumar B, Tiwari A (2005) Trifolium alexandrinum × T. resupinatum—interspecific hybrids developed through embryo rescue. Plant Cell Tissue Organ Cult 83:137–144CrossRefGoogle Scholar
  20. Lepiniec L, Debeaujon I, Routaboul J, Baudry A, Pourcel L, Nesi N, Caboche M (2006) Genetics and biochemistry of seed flavonoids. Annu Rev Plant Biol 57:405–430CrossRefGoogle Scholar
  21. Li YG, Tanner G, Larkin P (1996) The DMACA-HCl protocol and the threshold proanthocyanidin content for bloat safety in forage legumes. J Sci Food Agric 70:89–101CrossRefGoogle Scholar
  22. Li J, Chen L, Liang Y, Ye X, Liu L (2003) Research and commercial application of the complete dominance yellow-seeded gene in Brassica napus L. In: Proc. 11th International Rapeseed Congress, Copenhagen. Denmark vol 1, pp 202–204Google Scholar
  23. Li YH, Zhao SC, Ma JX, Li D, Yan L, Li J et al (2013) Molecular footprints of domestication and improvement in soybean revealed by whole genome re-sequencing. BMC Genom 2013:579.  https://doi.org/10.1186/1471-2164-14-579 CrossRefGoogle Scholar
  24. Maatooq GT (1997) Trifolexin: a new flavanonol derivative from Trifolium alexandrinum seeds. Mansoura J Pharm Sci 13:70–78Google Scholar
  25. Malaviya DR, Roy AK, Kaushal P, Kumar B, Tiwari A (2004a) Development and characterization of interspecific hybrids of Trifolium alexandrinum × T. apertum using embryo rescue. Plant Breed 123:536–542CrossRefGoogle Scholar
  26. Malaviya DR, Roy AK, Kaushal P, Tiwari A (2004b) Affinity between Trifolium alexandrinum and T. apertum—cytological investigation in embryo rescued hybrid. Cytologia 69:425–429CrossRefGoogle Scholar
  27. Malaviya DR, Kumar B, Roy AK, Kaushal P, Tiwari A (2005) Estimation of variability for isozymes of five enzyme systems among wild and cultivated species of Trifolium. Genet Resour Crop Evol 52:967–976CrossRefGoogle Scholar
  28. Malaviya DR, Roy AK, Kaushal P, Kumar B, Tewari A (2008) Genetic similarity among Trifolium species based on isozyme banding pattern. Plant Syst Evol 276:125–136CrossRefGoogle Scholar
  29. Malaviya DR, Yadav A, Roy AK, Kaushal P, Chakraborti M (2012) IL-11-239 (IC0593646; INGR12009), a Berseem (Trifolium alexandrinum) germplasm with Black (dark tan) seed against normal yellow seed. Indian J Plant Genet Resour 25:318–319Google Scholar
  30. Malaviya DR, Roy AK, Kaushal P, Chakraborti M, Yadav A, Khare A, Dhir R, Khairnar D, George GP (2018) Interspecific compatibility barriers, development of interspecific hybrids through embryo rescue and lineage of Trifolium alexandrinum (Egyptian clover)—important tropical forage legume. Plant Breed 137:655–672CrossRefGoogle Scholar
  31. Martínez CJ, Loarca-Pina G, Ortız GD (2003) Antimutagenic activity of phenolic compounds, oligosaccharides and quinolizidinic alkaloids from Lupinus campestris seeds. Food Addit Contam 20:940–948CrossRefGoogle Scholar
  32. Mohamed KM, Ohtani K, Kasai R, Yamasaki K (1995) Oleanene glycosides from seeds of Trifolium alexandrinum. Phytochemistry 40:1237–1242CrossRefGoogle Scholar
  33. Mohamed KM, Mohamed MH, Ohtani K, Kasai R, Yamasaki K (1999) Megastigmane glycosides from seeds of Trifolium alexandrinum. Phytochemistry 50:859–862CrossRefGoogle Scholar
  34. Mohamed KM, Hassaneana HA, Ohtanib K, Kasaib R, Yamasakib K (2000) Chalcanol glucosides from seeds of Trifolium alexandrinum. Phytochemistry 53:401–404CrossRefGoogle Scholar
  35. Nadkarni SR, Goud IS, Sheshaiah KC, Dalawai N, Hosamani M (2017) Genetics of seed colour in sunflower (Helianthus annuus L.). Int J Pure Appl Biosci 5:1207–1214.  https://doi.org/10.18782/2320-7051.2749 CrossRefGoogle Scholar
  36. Naumann HD, Muir JP, Lambert BD, Tedeschi LO, Kothmann MM (2013) Condensed tannins in the ruminant environment: a perspective on biological activity. J Agric Sci 1:8–20Google Scholar
  37. Ndakidemi PA, Dakora FD (2003) Legume seed flavonoids and nitrogenous metabolites as signals and protectants in early seedling development. Funct Plant Biol 30:729–745CrossRefGoogle Scholar
  38. Oigiangbe NO, Onigbinde AO (1996) The association between some physicochemical characteristics and susceptibility of cowpea (Vigna unguiculata (L.) Walp) to Callasobruchus maculates (F). J Stored Prod Res 32:7–11CrossRefGoogle Scholar
  39. Oppenheimer HR (1959) The origin of Egyptian clover with critical revision of some related species. Bull Res Counc Isr 7D:202–221Google Scholar
  40. Putiyevsky E, Katznelson J, Zohary M (1975) Cytogenetic studies in Trifolium spp. related to Berseem. IV. The relationships in the Alexandrinum and Vavilovii crossability groups and the origin of cultivated Berseem. Theor Appl Genet 45:355–362CrossRefGoogle Scholar
  41. Ricciardi L, Filippetti A, DePace C, Marzano CF (1985) Inheritance of seed coat colour in broad bean (Vicia faba L.). Euphytica 34:43–51CrossRefGoogle Scholar
  42. Roy AK, Malaviya DR, Kaushal P, Kumar B, Tiwari A (2004) Interspecific hybridization of T. alexandrinum with T. constantinopolitanum using embryo rescue. Plant Cell Rep 22:605–610CrossRefGoogle Scholar
  43. Sharaf M (2008) Chemical constituents from the seeds of Trifolium alexandrinum. Nat Prod Res 22:1620–1623CrossRefGoogle Scholar
  44. Slattery HD, Atwell BJ, Kuo J (1982) Relationship between colour, phenolic content and impermeability in the seed coat of various Trifolium subterraneum L. genotypes. Ann Bot 50:373–378CrossRefGoogle Scholar
  45. Slominski BA, Campbell LD, Guenter W (1994) Carbohydrates and dietary fiber components of yellow-seeded and brown-seeded canola. J Agric Food Chem 42:704–707CrossRefGoogle Scholar
  46. Sreenivasulu N, Wobus U (2013) Seed-development programs: a systems biology-based comparison between dicots and monocots. Annu Rev Plant Biol 64:189–217CrossRefGoogle Scholar
  47. Van Deynze AE, Beversdorf WD, Pauls KP (1993) Temperature effects on seed color in black- and yellow-seeded rapeseed. Can J Plant Sci 73:383–387CrossRefGoogle Scholar
  48. Velijević N, Štrbanović R, Poštić D, Stanisavljević R, Đukanović L (2017) Effects of seed coat colour on the seed quality and initial seedling growth of red clover cultivars (Trifolium pratense). J Process Energy Agric 21:174–177CrossRefGoogle Scholar
  49. Verdier J, Zhaoa J, Torres-Jereza I, Gea S, Liua C, Hea X, Mysore KS, Dixon RA, Udvardi MK (2012) MtPAR MYB transcription factor acts as an on switch for proanthocyanidin biosynthesis in Medicago truncatula. PNAS 109:1766–1771.  https://doi.org/10.1073/pnas.1120916109 CrossRefGoogle Scholar
  50. Verma P, Chandra A, Roy AK, Malaviya DR, Kaushal P, Pandey D, Bhatia S (2015) Development and characterization of genomic based SSR markers in berseem (Trifolium alexandrinum L.), an important multi-cut annual forage legume. Mol Breed 35:23.  https://doi.org/10.1007/s11032-015-0223-7 CrossRefGoogle Scholar
  51. Welinder KG (1992) Super family of plant, fungal and bacterial peroxidases. Curr Opin Struct Biol 2:388–393CrossRefGoogle Scholar
  52. Zhi-wen L, Ting-dong F, Jin-xing T, Bao-yuan C (2005) Inheritance of seed colour and identification of RAPD and AFLP markers linked to the seed colour gene in rapeseed (Brassica napus L.). Theor Appl Genet 110:303–310.  https://doi.org/10.1007/s00122-004-1835-1 CrossRefGoogle Scholar
  53. Zohary M (1972) Origin and evolution in the genus Trifolium. Bot Notiser 125:501–511Google Scholar
  54. Zohary M, Heller D (1984) The genus Trifolium. The Israel Academy of Sciences and Humanities, JerusalemGoogle Scholar
  55. Zoric L, Merkulov L, Lukovic J, Boza P (2010) Comparative seed morphology of Trifolium L. species (Fabaceae). Periodicum Biologorum 112:263–272Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.ICAR-Indian Grassland and Fodder Research InstituteJhansiIndia
  2. 2.ICAR-Indian Institute of Sugarcane ResearchLucknowIndia
  3. 3.ICAR-National Institute of Biotic Stress ManagementRaipurIndia

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