Abstract
The Cimbicidae is a small family of the primitive and relatively less diverse suborder Symphyta (Hymenoptera). Here, nearly complete mitochondrial genome (mitogenome) of hairy sawfly, Corynis lateralis (Hymenoptera: Cimbicidae) was sequenced using next generation sequencing and comparatively analysed with the mitogenome of Trichiosoma anthracinum. The sequenced length of C. lateralis mitogenome was 14,899 bp with an A+T content of 80.60%. All protein coding genes (PCGs) are initiated by ATN codons and all are terminated with TAR or T− stop codon. All tRNA genes preferred usual anticodons. Compared with the inferred insect ancestral mitogenome, two tRNA rearrangements were observed in the IQM and ARNS1EF gene clusters, representing a new event not previously reported in Symphyta. An illicit priming of replication and/or intra/inter-mitochondrial recombination and TDRL seem to be responsible mechanisms for the rearrangement events in these gene clusters. Phylogenetic analyses confirmed the position of Corynis within Cimbicidae and recovered a relationship of Tenthredinoidea + (Cephoidea + Orussoidea) in Symphyta.
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Acknowledgements
We thank TÜBİTAK (The Scientific and Technological Research Council of Turkey, project number 112T418) and Cumhuriyet University (CÜBAP F-395) for providing financial support. We are grateful to Dr. Hasan H. Başıbüyük (Department of Biology, Cumhuriyet University) for his critical reading and the improvement of the manuscript. We thank the anonymous reviewers for their constructive comments and suggestions. The members of Cumhuriyet University Molecular Systematics Research Group (CÜMSAG) are thanked for their contributions in laboratory work.
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Doğan, Ö., Korkmaz, E.M. Nearly complete mitogenome of hairy sawfly, Corynis lateralis (Brullé, 1832) (Hymenoptera: Cimbicidae): rearrangements in the IQM and ARNS1EF gene clusters. Genetica 145, 341–350 (2017). https://doi.org/10.1007/s10709-017-9969-7
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DOI: https://doi.org/10.1007/s10709-017-9969-7