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A comparative cytogenetic study of Drosophila parasitoids (Hymenoptera, Figitidae) using DNA-binding fluorochromes and FISH with 45S rDNA probe

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Abstract

Karyotypes of Leptopilina boulardi (Barbotin, Carton et Keiner-Pillault, 1979) (n = 9), L. heterotoma (Thomson, 1862) (n = 10), L. victoriae Nordlander, 1980 (n = 10) and Ganaspis xanthopoda (Ashmead, 1896) (n = 9) (Hymenoptera, Figitidae) were studied using DNA-binding ligands with different base specificity [propidium iodide (PI), chromomycin A3 (CMA3) and 4′,6-diamidino-2-phenylindole (DAPI)], and fluorescence in situ hybridization (FISH) with a 45S rDNA probe. Fluorochrome staining was similar between the different fluorochromes, except for a single CMA3- and PI-positive and DAPI-negative band per haploid karyotype of each species. FISH with 45S rDNA probe detected a single rDNA site in place of the bright CMA3-positive band, thus identifying the nucleolus organizing region (NOR). Chromosomal locations of NORs were similar for both L. heterotoma and L. victoriae, but strongly differed in L. boulardi as well as in G. xanthopoda. Phylogenetic aspects of NOR localization in all studied species are briefly discussed.

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Acknowledgments

We are grateful to Roma Rajwani for maintaining the laboratory stocks of parasitoids used in this study. The present work was supported by funds from the Russian Foundation for Basic Research (15-04-07709 to VEG, 14-08-01167 and 16-04-01239 to NLB and OVM) as well as from National Science Foundation (1121817), National Institute on Minority Health and Health Disparities (5G12MD007603-30) and National Aeronautical Space Agency (NNX15AB42G) to SG. The content does not represent the official views of the National Institute on Minority Health and Health Disparities or the National Institutes of Health.

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Authors and Affiliations

  1. Botanical Garden, Moscow State University, Moscow, Russia, 119234

    Vladimir E. Gokhman

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia, 119991

    Nadezhda L. Bolsheva & Olga V. Muravenko

  3. Biology Department MR526, The City College of the City University of New York, 138th Street and Convent Avenue, New York, NY, 10031, USA

    Shubha Govind

Authors
  1. Vladimir E. Gokhman
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  2. Nadezhda L. Bolsheva
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  3. Shubha Govind
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  4. Olga V. Muravenko
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Correspondence to Vladimir E. Gokhman.

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Gokhman, V.E., Bolsheva, N.L., Govind, S. et al. A comparative cytogenetic study of Drosophila parasitoids (Hymenoptera, Figitidae) using DNA-binding fluorochromes and FISH with 45S rDNA probe. Genetica 144, 335–339 (2016). https://doi.org/10.1007/s10709-016-9902-5

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