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Mating system and pollen dispersal in Eugenia dysenterica (Myrtaceae) germplasm collection: tools for conservation and domestication

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Abstract

Eugenia dysenterica DC. (Myrtaceae) is a perennial tree producing edible fruits and ornamental flowers of potential value widely distributed in Brazilian “Cerrados” (savannas), but available genetic resources and potential for future breeding programs must be evaluated. Here we evaluated the reproductive system and pollen-mediated gene flow in one generation of Eugenia dysenterica germplasm collection of Agronomy School, Federal University of Goiás (in Goiânia city, Central Brazil). We collected leaves from all adults from the germplasm collection (682 plants) and seeds (542) from 23 mother-trees. Genotypes were obtained for seven microsatellite loci. Genetic diversity was high and did not significantly differ between adults (H e  = 0.777) and progeny arrays (H e  = 0.617). Our results showed that E. dysenterica has an allogamous mating system in the germplasm collection (t m  = 0.957), but with high and significant biparental inbreeding (t m  − t s  = 0.109). Because sibs are very close to each other, mating between closely related individuals is likely. Paternity correlation was also relatively high, indicating a 11.9 % probability that a randomly chosen pair of outcrossed progeny from the same array are full sibs. The maximum pollen dispersal distance (224 m), estimated using assignment test, corresponded to the boundaries of the orchard. We were able to assign the paternity to only 64 % of the 349 seeds analyzed, indicating potential pollen immigration to the germplasm collection. The variance effective population size estimated for one maternal family in the germplasm collection (N ev  = 3.42) is very close to the theoretical maximum value for half-sibs (Nev = 4.0). Because E. dysenterica has a long life cycle and generation time, the maintenance of an effective population size of at least 100 in the germplasm collection is suggested, which can be achieved by maintaining a seed-trees number around 30 individuals.

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Acknowledgments

This work was supported by several grants and fellowships to the research network PRONEX ‘‘Núcleo de Excelência em Genética e Conservação de Espécies do Cerrado’’- GECER (PRONEX/FAPEG/CNPq CP 07-2009 e CP 07-2012), GENPAC (Geographical Genetics and Regional Planning for natural resources in Brazilian Cerrado) from CNPq/MCT/CAPES (563624/2010-8) and MCT/CNPq 14/2009—Universal (475182/2009-0). E.B.R. and L.R.M. received a CNPq scholarship. We thank everyone who worked in the last 20 years to Cerrado species at the Agronomy School of UFG, especially Dr. Ronaldo Veloso Naves. Work by M.P.C.T., R.G.C. and L.J.C. has been continuously supported by productivity fellowships from CNPq.

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  1. Programa de pós-graduação em Genética e Melhoramento de Plantas (PGMP), Escola de Agronomia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil

    Eduardo B. Rodrigues

  2. Laboratório de Genética & Biodiversidade, ICB, Universidade Federal de Goiás (UFG), Cx. P. 131, Goiânia, GO, 74001-970, Brazil

    Eduardo B. Rodrigues, Rosane G. Collevatti, Lucas R. Moreira & Mariana P. C. Telles

  3. Escola de Agronomia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil

    Lázaro J. Chaves

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  1. Eduardo B. Rodrigues
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Correspondence to Mariana P. C. Telles.

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Rodrigues, E.B., Collevatti, R.G., Chaves, L.J. et al. Mating system and pollen dispersal in Eugenia dysenterica (Myrtaceae) germplasm collection: tools for conservation and domestication. Genetica 144, 139–146 (2016). https://doi.org/10.1007/s10709-016-9884-3

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