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Phylogenetic analysis of PR genes in some pome fruit species with the emphasis on transcriptional analysis and ROS response under Erwinia amylovora inoculation in apple

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Abstract

Attempts were made to identify eight pathogenesis related (PR) genes (i.e., PR-1a, PR3-ch1, PR3-Ch2, PR3-Ch3, PR3-Ch4, PR3-Ch5, PR-5 and PR-8) from 27 genotypes of apple, quince and pear, which are induced in response to inoculation with the pathogen Erwinia amylovora, the causal agent of fire blight. Totally, 32 PR genes of different families were obtained, excepting PR3-Ch2 (amplified only in apple) and PR3-Ch4 (amplified only in apple and pear), the others were successfully amplified in all the genotypes of apple, quince and pear. Evolutionary, the genes of each family exhibited significant homology with each other, as the corresponded phylogenetic neighbor-joining-based dendrograms were taken into consideration. Meanwhile, according to the expression assay, it was deduced that the pathogen activity can significantly affect the expression levels of some selected PR genes of PR3-Ch2, PR3-Ch4, PR3-Ch5 and particularly Cat I in both resistant (MM-111) and semi-susceptible (MM-106) apple rootstocks. Lastly, it was concluded that the pathogen E. amylovora is able to stimulate ROS response, particularly using generation of hydrogen peroxide (H2O2) in both aforementioned apple rootstock.

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Author contributions

MH participated in performing the experimental work, molecular section. MRS participated in carrying out the Real time section of the work and assisted in manuscript revising. JN performed all the bioinformatics and data analysis, provided the manuscript draft and revised its final version. HA designed and supervised molecular section of the study, revised the manuscript and provided helpful discussions. MG participated in performing the experimental work, Reactive oxygen species (ROS) assay section. All the authors read and approved final version of the manuscript.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Agriculture and Natural Resources, Azad University (Science and Research Branch), P.O. Box 4933, Tehran, 14155, Iran

    Maryam Hassani & Zahra Ghahremani

  2. Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, P.O. Box 4111, Karaj, 31587, Iran

    Seyed Alireza Salami

  3. Department of Agronomy and Plant Breeding, Division of Molecular Plant Genetics, College of Agricultural & Natural Resources, University of Tehran, Karaj, Tehran, Iran

    Jaber Nasiri

  4. Department of Horticulture Research, Seed and Plant Improvement Institute, P.O. Box 4119, Karaj, 31585, Iran

    Hamid Abdollahi

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  1. Maryam Hassani
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  2. Seyed Alireza Salami
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  3. Jaber Nasiri
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  4. Hamid Abdollahi
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  5. Zahra Ghahremani
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Correspondence to Jaber Nasiri or Hamid Abdollahi.

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Hassani, M., Salami, S.A., Nasiri, J. et al. Phylogenetic analysis of PR genes in some pome fruit species with the emphasis on transcriptional analysis and ROS response under Erwinia amylovora inoculation in apple. Genetica 144, 9–22 (2016). https://doi.org/10.1007/s10709-015-9874-x

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  • DOI: https://doi.org/10.1007/s10709-015-9874-x

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