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Genetica

, Volume 143, Issue 4, pp 393–401 | Cite as

Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats

  • Renzo Sebastián Adilardi
  • Andrés Alejandro Ojanguren-Affilastro
  • Camilo Iván Mattoni
  • Liliana María Mola
Article

Abstract

All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n = 18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG) n motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism.

Keywords

Holokinetic chromosomes Achiasmatic meiosis Telomere FISH Female diffuse stage 

Notes

Acknowledgments

This work was supported by grants from the National Council of Scientific and Technological Research (CONICET) (PIP 0342), University of Buenos Aires (UBA) (Ex 0859) and National Agency for Scientific and Technological Promotion (ANPCyT) (PICT 2010-1665) to Drs L. Poggio and L. Mola, ANPCyT (PICT 2010-0906) to Drs A. V. Peretti and C. I. Mattoni and ANPCyT (PICT 2010-1764) to Dr A. A. Ojanguren-Affilastro. The authors wish to thank to Gonzalo Rubio, Andrés Porta, Ernesto Costa-Schmidt and Hernán Iuri for their help during the field work.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Renzo Sebastián Adilardi
    • 1
  • Andrés Alejandro Ojanguren-Affilastro
    • 2
  • Camilo Iván Mattoni
    • 3
  • Liliana María Mola
    • 1
  1. 1.Laboratorio de Citogenética y Evolución, Departamento de Ecología, Genética y Evolución, IEGEBA (CONICET-UBA), Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresCABAArgentina
  2. 2.División de AracnologíaMuseo Argentino de Ciencias Naturales “Bernardino Rivadavia”, CONICETCABAArgentina
  3. 3.Laboratorio de Biología Reproductiva y Evolución (CONICET-UNC), Facultad de Ciencias Exactas, Físicas y NaturalesUniversidad Nacional de CórdobaCórdobaArgentina

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