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Distribution of 18S rDNA sites and absence of the canonical TTAGG insect telomeric repeat in parasitoid Hymenoptera

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Abstract

Karyotypes of six species belonging to three main clades of parasitoid Hymenoptera, the superfamilies Ichneumonoidea (Ichneumonidae: Ichneumon amphibolus), Cynipoidea (Cynipidae: Diplolepis rosae) and Chalcidoidea (Eurytomidae: Eurytoma robusta, Eu. serratulae and Eu. compressa, and Torymidae: Torymus bedeguaris) were studied using FISH with 18S rDNA and telomeric (TTAGG)n probes. Haploid karyotypes of D. rosae, Eu. robusta and Eu. serratulae carried the only 18S rDNA hybridization signal, whereas those of I. amphibolus and Eu. compressa carried three and two rDNA clusters respectively. In addition, three rDNA sites were visualized in the aneuploid female of T. bedeguaris. The number of rDNA clusters in parasitoid Hymenoptera generally correlates to the chromosome number. Apart from the overwhelming majority of the studied species of aculeate Hymenoptera, no hybridization signals were obtained from FISH with the telomeric (TTAGG)n probe in the examined parasitoid species. These data suggest absence of the canonical (TTAGG)n insect telomeric motif in the Ichneumonoidea, Cynipoidea and Chalcidoidea, and perhaps in parasitoid Hymenoptera in general.

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Acknowledgments

The present study was partly supported by the research Grant No. 14-04-01051 from the Russian Foundation for Basic Research (RFBR) and programs of the Presidium of the Russian Academy of Sciences “Gene Pools and Genetic Diversity” and “Origin of the Biosphere and Evolution of Geo-Biological Systems”. Dr. Robert B. Angus (School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK) has kindly checked the language of the manuscript.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Botanical Garden, Moscow State University, Moscow, 119234, Russia

    Vladimir E. Gokhman

  2. Zoological Institute, Russian Academy of Sciences, St. Petersburg, 199034, Russia

    Boris A. Anokhin & Valentina G. Kuznetsova

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  1. Vladimir E. Gokhman
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  2. Boris A. Anokhin
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  3. Valentina G. Kuznetsova
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Correspondence to Vladimir E. Gokhman.

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Gokhman, V.E., Anokhin, B.A. & Kuznetsova, V.G. Distribution of 18S rDNA sites and absence of the canonical TTAGG insect telomeric repeat in parasitoid Hymenoptera. Genetica 142, 317–322 (2014). https://doi.org/10.1007/s10709-014-9776-3

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  • DOI: https://doi.org/10.1007/s10709-014-9776-3

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