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Genetic analysis and molecular mapping of a new fertility restorer gene Rf8 for Triticum timopheevi cytoplasm in wheat (Triticum aestivum L.) using SSR markers

Genetica volume 141, pages 431–441 (2013)Cite this article

Abstract

A study on mode of inheritance and mapping of fertility restorer (Rf) gene(s) using simple sequence repeat (SSR) markers was conducted in a cross of male sterile line 2041A having Triticum timopheevi cytoplasm and a restorer line PWR4099 of common wheat (Triticum aestivum L.). The F1 hybrid was completely fertile indicating that fertility restoration is a dominant trait. Based on the pollen fertility and seed set of bagged spikes in F2 generation, the individual plants were classified into fertile and sterile groups. Out of 120 F2 plants, 97 were fertile and 23 sterile (based on pollen fertility) while 98 plants set ≥5 seeds/spike and 22 produced ≤4 or no seed. The observed frequency fits well into Mendelian ratio of 3 fertile: 1 sterile with χ2 value of 2.84 for pollen fertility and 2.17 for seed setting indicating that the fertility restoration is governed by a single dominant gene in PWR4099. The three linked SSR markers, Xwmc503, Xgwm296 and Xwmc112 located on the chromosome 2DS were placed at a distance of 3.3, 5.8 and 6.7 cM, respectively, from the Rf gene. Since, no known Rf gene is located on the chromosome arm 2DS, the Rf gene in PWR4099 is a new gene and proposed as Rf8. The closest SSR marker, Xwmc503, linked to the Rf8 was validated in a set of Rf, maintainer and cytoplasmic male sterile lines. The closely linked SSR marker Xwmc503 may be used in marker-assisted backcross breeding facilitating the transfer of fertility restoration gene Rf8 into elite backgrounds with ease.

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Acknowledgments

Financial support provided by the Council of Scientific and Industrial Research (CSIR), New Delhi, India in the form of Emeritus Scientist scheme to SMST is gratefully acknowledged. The  study  is  part  of  the  Ph.D.  research of  the  first  author. The authors are also grateful to the Director, Indian Agricultural Research Institute, New Delhi for providing facilities to conduct research work in the Division of Genetics.

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Authors and Affiliations

  1. Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110012, India

    Pallavi Sinha, S. M. S. Tomar,  Vinod & Vikas K. Singh

  2. Department of Genetics and Plant Breeding, Chaudharay Charan Singh University, Meerut, 200005, India

    Pallavi Sinha & H. S. Balyan

  3. Centre of Excellence in Genomics, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, 502324, India

    Pallavi Sinha

Authors
  1. Pallavi Sinha
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  2. S. M. S. Tomar
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  3. Vinod
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  4. Vikas K. Singh
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  5. H. S. Balyan
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Correspondence to Pallavi Sinha.

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Sinha, P., Tomar, S.M.S., Vinod et al. Genetic analysis and molecular mapping of a new fertility restorer gene Rf8 for Triticum timopheevi cytoplasm in wheat (Triticum aestivum L.) using SSR markers. Genetica 141, 431–441 (2013). https://doi.org/10.1007/s10709-013-9742-5

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  • DOI: https://doi.org/10.1007/s10709-013-9742-5

Keywords

  • Hybrid wheat
  • Fertility restorer gene
  • Bulked segregant analysis (BSA)
  • Molecular mapping

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