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Variations of chromosomal structures in Caluromys philander (Didelphimorphia: Didelphidae) from the Amazon region

Abstract

Caluromys is considered to be one of the most ancient genera of extant marsupials and is positioned among the basal taxa of the family Didelphidae. At least two species occur in Brazil, C. philander and C. lanatus, both of which have 2n = 14 chromosomes. For the first time, we present evidence of an intrapopulation polymorphism of the sexual chromosome pair in C. philander females from the Central Amazon region. Detailed cytogenetic results of animals from three localities on the Amazon region were analyzed using classical cytogenetics (NOR, C-Band and G-Band) and molecular techniques (18S rDNA and telomere probes). Similar to other conspecific individuals, the diploid number of these animals is 2n = 14, and their fundamental number is 24, with NOR present on the 6th autosomal pair. The X chromosome presented variation detectable by G banding, suggesting a pericentric inversion.

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References

  • Biggers JD, Fritz WC, Hare WCD, McFeely RA (1965) Chromosomes of American marsupials. Science 148:1602–1603

    CAS  Article  Google Scholar 

  • Carvalho BA, Mattevi MS (2000) (T2AG3)n telomeric sequence hybridization suggestive of centric fusion in karyotype marsupials evolution. Genetica 108:205–210

    CAS  Article  Google Scholar 

  • Carvalho BA, Oliveira LF, Nunes AP, Mattevi MS (2002) Karyotypes of nineteen marsupial species from Brazil. J Mammal 83(1):58–70

    Article  Google Scholar 

  • Casartelli C, Rogatto SR, Ferrari I (1986) Cytogenetic analysis of some Brazilian marsupials (Didelphidae: Marsupialia). Can J Genet Cytol 28:21–29

    CAS  Article  Google Scholar 

  • Da Silva MNF, Arteaga MC, Bantel CG, Rossoni DM, Leite RN, Pinheiro PSF, Röhe PS, Eler ES (2007) Mamíferos de pequeno porte (Mammalia: Rodentia & Didelphimorphia). In: Rapp Py-Daniel L, Deus CP, Henriques AL, Pimpão DM, Ribeiro OM (eds) Biodiversidade do Médio madeira: bases científicas para propostas de conservação. Instituto Nacional de Pesquisas da Amazônia, Manaus, pp 179–194

    Google Scholar 

  • Fantin C, da Silva MNF (2011) The Karyotype of a rare South American marsupial. The bushy-tailed opossum genus Glironia (Didelphimorphia: Didelphidae). Mastozoologia Neotropical 18(1):125–130

    Google Scholar 

  • Ford C, Hamerton J (1956) A colchicine hypothonic citrate squash sequence for mammalian chromosomes. Stain Technol 31:247–251

    CAS  Article  Google Scholar 

  • Gardner AL, Patton JL (1976) Karyotipic variation in Oryzomine rodents (Cricetidae) with comments on chromosomal evolution in the Neotropical Cricetinae complex. Occas Pap Mus Zool 49:1–48

    Google Scholar 

  • Gold JR, Li YC, Shipley NS, Powers PK (1990) Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. J Fish Biol 37:563–575

    Article  Google Scholar 

  • Gross MC, Schneider CH, Valente GT, Martins C, Feldberg E (2010) Variability of 18S rDNA locus among Symphysodon fishes: chromosomal rearrangements. J Fish Biol 76:1117–1127

    CAS  Article  Google Scholar 

  • Hayman DL, Martin G (1974) Mammalia I: Monotremata and Marsupialia, Chordata 4. In: John B (ed) Animal Cytogenetics. Berlin. Pp, Gebrüder Borntraeger, pp 1–110

    Google Scholar 

  • Howel WM, Black DA (1980) Controlled silver-staining of nucleolus organizer region with a protective colloidal developer: a 1-step method. Experientia 36:1014–1015

    Article  Google Scholar 

  • Ijdo JW, Wells RA, Baldini A, Reeders ST (1991) Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res 19(17):47–80

    Article  Google Scholar 

  • Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220

    Article  Google Scholar 

  • O’Neill RJ, Eldridge MDB, Metcalf CJ (2004) Centromere dynamics and chromosome evolution in marsupials. J Hered 95(5):375–381

    Article  Google Scholar 

  • Page J, Berríos S, Parra MT, Vieira A, Suja JA, Prieto I, Barvero JL, Rufas JS, Donoso RF (2005) The program of sex chromosome pairing in meiosis is highly conserved across marsupial species: implications for sex chromosome evolution. Genetics 170:793–799

    CAS  Article  Google Scholar 

  • Pagnozzi JM, Silva MJJ, Yonenaga-Yassuda Y (2000) Intraspecific variation in the distribution of the interstitial telomeric (TTAGGG)n sequences in Micoureus demerarae (Marsupialia, Didelphidae). Chromosome Res 8:585–591

    CAS  Article  Google Scholar 

  • Palma RE, Yates TL (1996) The chromosomes of the Bolivian didelphid marsupials. Occas Pap Mus Tex Tech Univ 162:1–20

    Google Scholar 

  • Patton JL, Costa LP (2006) Diversidade e limites geográficos e sistemáticos de marsupiais brasileiros. In: Caceres NC, Monteiro-Filho ELA (eds) Os marsupiais do Brasil: biologia, ecologia e evolução. Universidade Federal do Mato Grosso do Sul, Campo Grande, pp 287–326

    Google Scholar 

  • Patton JL, da Silva MNF, Malcolm JR (2000) Mammals of the rio Juruá and the evolutionary and ecological diversification of Amazonia. Bull Amer Mus Nat Hist 244:1–306

    Article  Google Scholar 

  • Pereira NP, Ventura V, Silva MCJ, Silva DM, Yonenaga-Yassuda Y, Pellegrino CM (2008) Karyotype characterization and nucleolar organizer regions of marsupial species (Didelphidae) from areas of Cerrado and Atlantic Forest in Brazil. Genet Mol Biol 31(4):887–892

    Article  Google Scholar 

  • Pinkel D, Straume D, Gray JW (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci 83:2934–2938

    CAS  Article  Google Scholar 

  • Reig OA, Gardner AL, Bianchi NO, Patton JL (1977) The chromosomes of Didelphidae (Marsupialia) and their evolutontionary significance. Biol J Linn Soc 9:191–216

    Article  Google Scholar 

  • Rofe R, Hayman D (1985) G-banding evidence for a conserved complement in the Marsupialia. Cytogenet Cell Genet 39:40–50

    CAS  Article  Google Scholar 

  • Sharp P (1982) Sex chromosome pairing during male meiosis in marsupials. Chromosoma 86:27–47

    CAS  Article  Google Scholar 

  • Souza MJ, Maia V, Santos JF (1990) Nucleolar organizer regions, G- and C-band in some Brazilian species of Didelphidae. Rev Bras Genet 13:767–775

    Google Scholar 

  • Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304–306

    CAS  Article  Google Scholar 

  • Svartman M (2009) American marsupials chromosomes: why study them? Genet Mol Biol 32(4):675–687

    CAS  Article  Google Scholar 

  • Svartman M, Vianna-Morgante AM (1998) Karyotype evolution of marsupials: from higher to lower diploid numbers. Cytogenet Cell Genet 82:263–266

    CAS  Article  Google Scholar 

  • Svartman M, Vianna-Morgante AM (1999) Comparative genome analysis in American marsupials: chromosome banding and in situ hybridization. Chromosome Res 7:267–275

    CAS  Article  Google Scholar 

  • Svartman M, Vianna-Morgante AM (2003) Conservation of chromosomal location of nucleolus organizer in American marsupials (Didelphidae). Genetica 118:11–16

    CAS  Article  Google Scholar 

  • Voss RS, Jansa S (2009) Phylogenetic relationships and classification of didelphid marsupials, an extant radiation of new world metatherian mammals. Bull Am Mus Nat Hist 322:1–177

    Article  Google Scholar 

  • Wilson DE, Reeder DM (2005) Mammal species of the world. Johns Hopkins University Press, Canada

    Google Scholar 

Download references

Acknowledgments

The authors would like to thank RA Andrade and MAA Schetino for their help with field work; CH Schneider and MC Gross for their assistance in the laboratory and helpful tips. This work was supported by the Instituto Nacional de Pesquisas da Amazônia (INPA) through the Research Institutional Projects (PRJ.05-73), Petrobrás S/A. EMS Souza - received a studentship from the Fundação de Amparo à Pesquisa no Amazonas (FAPEAM).

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Correspondence to Érica Martinha Silva de Souza.

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de Souza, É.M.S., e Silva, C.E.F., Eler, E.S. et al. Variations of chromosomal structures in Caluromys philander (Didelphimorphia: Didelphidae) from the Amazon region. Genetica 141, 89–93 (2013). https://doi.org/10.1007/s10709-013-9708-7

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  • DOI: https://doi.org/10.1007/s10709-013-9708-7

Keywords

  • Cytogenetic
  • FISH
  • Marsupial
  • Rearrangement