Genetica

, Volume 140, Issue 10–12, pp 497–504 | Cite as

Genome size and chromosome number in velvet worms (Onychophora)

  • Nicholas W. Jeffery
  • Ivo S. Oliveira
  • T. Ryan Gregory
  • David M. Rowell
  • Georg Mayer
Article

Abstract

The Onychophora (velvet worms) represents a small group of invertebrates (~180 valid species), which is commonly united with Tardigrada and Arthropoda in a clade called Panarthropoda. As with the majority of invertebrate taxa, genome size data are very limited for the Onychophora, with only one previously published estimate. Here we use both flow cytometry and Feulgen image analysis densitometry to provide genome size estimates for seven species of velvet worms from both major subgroups, Peripatidae and Peripatopsidae, along with karyotype data for each species. Genome sizes in these species range from roughly 5–19 pg, with densitometric estimates being slightly larger than those obtained by flow cytometry for all species. Chromosome numbers range from 2n = 8 to 2n = 54. No relationship is evident between genome size, chromosome number, or reproductive mode. Various avenues for future genomic research are presented based on these results.

Keywords

C-value Nuclear DNA content Peripatidae Peripatopsidae Flow cytometry Feulgen image analysis densitometry Karyotype 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Nicholas W. Jeffery
    • 1
  • Ivo S. Oliveira
    • 2
  • T. Ryan Gregory
    • 1
  • David M. Rowell
    • 3
  • Georg Mayer
    • 2
  1. 1.Department of Integrative BiologyUniversity of GuelphGuelphCanada
  2. 2.Animal Evolution and Development, Institute of BiologyUniversity of LeipzigLeipzigGermany
  3. 3.Division of Evolution, Ecology and Genetics, Research School of BiologyAustralian National UniversityCanberraAustralia

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